Results 211 to 220 of about 587,888 (232)

In Situ Hybridization for RNA: Nonradioactive Probe: RNA Probe

2000
Non-radioactive in situ hybridization method is now widely used to detect nucleic acid in tissue sections. However, the results for the detection of tissue mRNA are not always consistent. We have to consider several factors for this purpose. We describe our current methods for the most consistent detection.
openaire   +2 more sources

Terbium as a solid-state probe for RNA

Bioinorganic Chemistry, 1978
This paper continues previous work on the analysis of nucleic acid-terbium complexes in the solid state. The fluorescence excitation and emission spectra of the RNA-terbium(III) complex is reported. The fluorescence excitation and emission spectra of both the RNA-terbium(III) and DNA-terbium(III) complexes as trapped on millipore filters is reported ...
George F. Vande Woude, George W. Mushrush, Gerry Robey, Donald Helman, Gene Yonuschot   +4 more
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Antisense probing of dynamic RNA structures

Methods, 2020
RNA regulation is influenced by the dynamic changes in conformational accessibility on the transcript. Here we discuss the initial validation of a cell-free antisense probing method for structured RNAs, using the Tetrahymena group I intron as a control target.
Alexandra J. Lukasiewicz, Lydia M. Contreras   +1 more
openaire   +3 more sources

Modern methods for probing RNA structure

Current Opinion in Biotechnology, 1998
Molecular biologists have been remarkably successful in dividing large RNAs into small functional modules manageable for NMR and X-ray studies. At the same time biophysical, biochemical and genetic tools in RNA structure determination have reached a level of sophistication, at which we start to see a glimpse of molecular dynamics and the mechanism of ...
Jan Egebjerg, Jørgen Kjems
openaire   +3 more sources

The RNA structurome: high-throughput probing

Nature Methods, 2010
Novel deep-sequencing strategies are used to monitor, at the genomic scale, the structure of cellular RNAs using enzymatic probing.
Westhof, Eric, Romby, Pascale
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RNA Remodeling by RNA Chaperones Monitored by RNA Structure Probing

2019
RNA structure probing enables the characterization of RNA secondary structures by established procedures such as the enzyme- or chemical-based detection of single- or double-stranded regions. A specific type of application involves the detection of changes of RNA structures and conformations that are induced by proteins with RNA chaperone activity ...
Tobias Schmidt, Sven-Erik Behrens, Susann Friedrich   +2 more
openaire   +3 more sources

Use of RNA Probes to Detect Plant RNA Viruses

1986
Publisher Summary This chapter discusses the use of RNA probes to detect plant RNA viruses. The detection and diagnosis of plant viral disease agents by nucleic acid hybridization is a valuable technique. This simple and sensitive hybridization assay permits the rapid analysis of a large number of individual plant samples for viral and viral-like ...
T.H. Turpen, S.J. Garger
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Genetic probes of ribosomal RNA function

Biochemistry and Cell Biology, 1995
We have used a genetic approach to uncover the functional roles of rRNA in protein synthesis. Mutations were constructed in a cloned rrn operon by site-directed mutagenesis or isolated by genetic selections following random mutagenesis. We have identified mutations that affect each step in the process of translation.
Steven T. Gregory, Albert E. Dahlberg, Matthew A. Firpo, J. S. Lodmell, C. A. Brunelli, D. I. Van Ryk, Michael O'Connor, Kathy R. Lieberman, Hervé Moine   +8 more
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RNA stable-isotope probing

Nature Protocols, 2007
Stable-isotope probing is a method used in microbial ecology that provides a means by which specific functional groups of organisms that incorporate particular substrates are identified without the prerequisite of cultivation. Stable-isotope-labeled carbon (13C) or nitrogen (15N) sources are assimilated into microbial biomass of environmental samples ...
Neufeld, Josh D., Vohra, Jyotsna, Dumont, Marc G., Lueders, Tillmann, Manefield, Mike, Friedrich, Michael W., Murrell, J. Colin   +6 more
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Solution Structure Probing of RNA Structures

2008
Single-stranded RNA plant viruses not only code for viral proteins within their RNA genomes, they often maintain elaborate RNA secondary structures. These structures can be integral to a variety of viral processes, such as viral translation, genome replication, subgenomic mRNA transcription, and genome encapsidation.
Marc R. Fabian, K. Andrew White
openaire   +3 more sources