Results 261 to 270 of about 1,213,585 (311)
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Sequencing diverse RNA modifications
Nature Methods, 2021Nanopore sequencing enables quantitative profiling of pseudouridine and 2′-O-methylation modifications in native RNAs.
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Nature Biotechnology, 2014
Using a cell as an RNA sequencing chip enables spatial analyses of the transcriptome at subcellular resolution.
Paul, Ginart, Arjun, Raj
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Using a cell as an RNA sequencing chip enables spatial analyses of the transcriptome at subcellular resolution.
Paul, Ginart, Arjun, Raj
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Paramecium: RNA sequence–structure phylogenetics
International Journal of Systematic and Evolutionary Microbiology, 2023Organisms classified as members of the genus Paramecium belong to the best-known group of single-celled eukaryotes. Nevertheless, the phylogeny within the genus Paramecium has been discussed and revisited in recent decades and remains partly unresolved.
Marlyn Weimer +2 more
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Identification of Circular RNAs using RNA Sequencing
Journal of Visualized Experiments, 2019Circular RNAs (circRNAs) are a class of non-coding RNAs involved in functions including micro-RNA (miRNA) regulation, mediation of protein-protein interactions, and regulation of parental gene transcription. In classical next generation RNA sequencing (RNA-seq), circRNAs are typically overlooked as a result of poly-A selection during construction of ...
Shobana, Sekar +6 more
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Depletion of Ribosomal RNA Sequences from Single‐Cell RNA‐Sequencing Library
Current Protocols in Molecular Biology, 2016AbstractRecent advances in single‐cell RNA sequencing technologies have revealed high heterogeneity of gene expression profiles in individual cells. However, most current single‐cell RNA‐seq methods use oligo‐dT priming in the reverse transcription steps and detect only polyA‐positive for more accuracy, since there are also polyA‐positive non‐coding ...
Nan, Fang, Rumeysa, Akinci-Tolun
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Transcriptome Sequencing: RNA-Seq
2018RNA sequencing (RNA-seq) can not only be used to identify the expression of common or rare transcripts but also in the identification of other abnormal events, such as alternative splicing, novel transcripts, and fusion genes. In principle, RNA-seq can be carried out by almost all of the next-generation sequencing (NGS) platforms, but the libraries of ...
Hong, Zhang, Lin, He, Lei, Cai
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Maternal plasma RNA sequencing
Clinical Biochemistry, 2015This review discusses the question: which steps are essential or need to be optimized so that maternal plasma RNA sequencing for pre-eclampsia and related syndromes will become as specific and sensitive as maternal plasma DNA sequencing for trisomy?
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RNA-Sequencing of Staphylococcus aureus Messenger RNA
2014RNA-sequencing (RNA-seq) is a technique that employs next-generation DNA-sequencing technology to simultaneously sequence all of the RNA transcripts in a cell. It can provide valuable insights into transcript and operon structure, and is rapidly replacing expression microarrays as the technique of choice for determining global gene expression profiles ...
Ronan K, Carroll +2 more
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