Results 271 to 280 of about 1,213,585 (311)

Sequence Design for RNA-RNA Interactions

The design of RNA sequences with desired structural properties presents a challenging computational problem with promising applications in biotechnology and biomedicine. Most regulatory RNAs function by forming RNA-RNA interactions, e.g., in order to regulate mRNA expression.
Maria Waldl, Hua-Ting Yao, Ivo L. Hofacker   +2 more
openaire   +2 more sources

RNA sequencing

1990
Abstract The first complete nucleotide sequence of a gene was published in 1964 by Robert Holley and associates. (1). This sequence was inferred from the transcript of the gene encoding yeast alanine tRNA. Determination of the 77 nucleotide sequence required 1 g of alanine tRNA, recovered from. 200 g of bulk tRNA, isolated from 140 kg
openaire   +1 more source

Enhancing Diagnosis Through RNA Sequencing

Clinics in Laboratory Medicine, 2020
The diagnostic rate of comprehensive genomic sequencing remains only 25% to 30% due to the difficulty in interpreting variants of uncertain significance and noncoding mutations and in elucidating downstream effects of these and other genetic changes. Unlike DNA sequencing, RNA sequencing (RNAseq) reveals the functional consequence of genetic variation ...
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RNA Sequencing Protocols for Short-Read Sequencing

RNA sequencing (RNA-seq) methodologies allow the discovery of novel variants and transcripts. These comprise three general steps: (1) capture of RNA species of interest, (2) conversion of RNA to complementary DNA (cDNA), and (3) modification of cDNA to fit the sequencing platform.
Laura, Vasquez-Velez, Veera, D'Mello, Patricia, Soteropoulos   +2 more
openaire   +2 more sources

RNA Sequencing by Primer Extension

Cold Spring Harbor Protocols, 2013
Direct sequencing of RNA by primer extension is a fast and accurate method that is useful in a variety of situations. It is a valuable technique for determining the faithfulness of in vitro processing reactions, such as splicing or RNA editing. It is often used as an alternative to reverse transcription PCR (RT–PCR) followed by cloning and DNA ...
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Nuclear RNA Isolation and Sequencing

2016
Most transcriptome studies involve sequencing and quantification of steady-state mRNA by isolating and sequencing poly (A) RNA. Although this type of sequencing data is informative to determine steady-state mRNA levels it does not provide information on transcriptional output and thus may not always reflect changes in transcriptional regulation of gene
Navroop K, Dhaliwal, Jennifer A, Mitchell   +1 more
openaire   +3 more sources

Cancer epigenetics in clinical practice

Ca-A Cancer Journal for Clinicians, 2023
Veronica Davalos, Manel Esteller
exaly  

Systemic therapy for hormone receptor‐positive/human epidermal growth factor receptor 2‐negative early stage and metastatic breast cancer

Ca-A Cancer Journal for Clinicians, 2023
Laura A Huppert, Hope S Rugo
exaly  

RNA Sequencing (RNA-seq)

2021
openaire   +1 more source

Hierarchical Nanomaterials Assembled from Peptoids and Other Sequence-Defined Synthetic Polymers

Chemical Reviews, 2021
Zhiliang Li, Bin Cai, Wenchao Yang
exaly