Results 81 to 90 of about 1,809,694 (326)

Deciphering the RNA landscape by RNAome sequencing [PDF]

RNA Biology, 2015
Current RNA expression profiling methods rely on enrichment steps for specific RNA classes, thereby not detecting all RNA species in an unperturbed manner. We report strand-specific RNAome sequencing that determines expression of small and large RNAs from rRNA-depleted total RNA in a single sequence run. Since current analysis pipelines cannot reliably
Derks, K.W.J., Misovic, B., Hout, M.C.G.N. van den, Kockx, C.E.M., Gomez, C.P., Brouwer, R.W.W., Vrieling, H., Hoeijmakers, J.H.J., IJcken, W.F.J. van, Pothof, J.   +9 more
openaire   +4 more sources

Use of RNA secondary structure for evolutionary relationships : investigating RNase P and RNase MRP : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Genetics at Massey University, New Zealand [PDF]

, 1998
Bioinformatics is applied here to examine whether RNA secondary structure data can reflect distant evolutionary relationships. This is important when there is little confidence in sequence data such as when looking at the evolution of RNase MRP (MRP ...
Collins, Lesley Joan
core  

Purification tags markedly affect self‐aggregation of CPEB3

FEBS Letters, EarlyView.
Although recombinant proteins are used to study protein aggregation in vitro, uncleaved tags can interfere with accurate interpretation. Our findings demonstrate that His₆‐GFP and His₁₂ tags significantly affect liquid droplet and amyloid fibril formation in the intrinsically disordered region (IDR) of mouse cytoplasmic polyadenylation element‐binding ...
Harunobu Saito, Yujin Lee, Motoharu Ueno, Naotaka Sekiyama, Masatomo So, Ayako Furukawa, Kenji Sugase   +6 more
wiley   +1 more source

Recognition of two distinct elements in the RNA substrate by the RNA-binding domain of the T. thermophilus DEAD box helicase Hera [PDF]

, 2013
DEAD box helicases catalyze the ATP-dependent destabilization of RNA duplexes. Whereas duplex separation is mediated by the helicase core shared by all members of the family, flanking domains often contribute to binding of the RNA substrate.
Klostermeier, Dagmar, Linden, Martin H., Rudolph, Markus G., Steimer, Lenz, Wurm, Jan Philip, Wöhnert, Jens   +5 more
core   +1 more source

Secondary-Structure-Informed RNA Inverse Design via Relational Graph Neural Networks

Non-Coding RNA
RNA inverse design is an essential part of many RNA therapeutic strategies. To date, there have been great advances in computationally driven RNA design.
Amirhossein Manzourolajdad, Mohammad Mohebbi   +1 more
doaj   +1 more source

Insights into pegRNA design from editing of the cardiomyopathy‐associated phospholamban R14del mutation

FEBS Letters, EarlyView.
This study reveals how prime editing guide RNA (pegRNA) secondary structure and reverse transcriptase template length affect prime editing efficiency in correcting the phospholamban R14del cardiomyopathy‐associated mutation. Insights support the design of structurally optimized enhanced pegRNAs for precise gene therapy.
Bing Yao, Qiangbing Yang, Christian J.B. Snijders Blok, Mark A. Daniels, Pieter A. Doevendans, Raymond Schiffelers, Joost P.G. Sluijter, Zhiyong Lei   +7 more
wiley   +1 more source

The elemental mechanism of transcriptional pausing. [PDF]

, 2019
Transcriptional pausing underlies regulation of cellular RNA biogenesis. A consensus pause sequence that acts on RNA polymerases (RNAPs) from bacteria to mammals halts RNAP in an elemental paused state from which longer-lived pauses can arise.
Chua, Xien Yu, Landick, Robert, Mishanina, Tatiana V, Mooney, Rachel Anne, Nayak, Dhananjaya, Saba, Jason, Windgassen, Tricia A   +6 more
core   +1 more source

Über die Sequenzanalyse an Ribonukleinsäuren

CHIMIA, 1971
Methods used for the nucleotide sequence determination of ribonucleic acids are reviewed. Special emphasis is given to a new approach to the sequence determination of bacteriophage Qß RNA using enzymatically synthesized RNA. 
Martin A. Billeter
doaj   +1 more source

Single‐cell insights into the role of T cells in B‐cell malignancies

FEBS Letters, EarlyView.
Single‐cell technologies have transformed our understanding of T cell–tumor cell interactions in B‐cell malignancies, revealing new T‐cell subsets, functional states, and immune evasion mechanisms. This Review synthesizes these findings, highlighting the roles of T cells in pathogenesis, progression, and therapy response, and underscoring their ...
Laura Llaó‐Cid
wiley   +1 more source

RCARE: RNA Sequence Comparison and Annotation for RNA Editing [PDF]

BMC Medical Genomics, 2015
The post-transcriptional sequence modification of transcripts through RNA editing is an important mechanism for regulating protein function and is associated with human disease phenotypes. The identification of RNA editing or RNA-DNA difference (RDD) sites is a fundamental step in the study of RNA editing.
Chan Hee Park, Je-Gun Joung, Soo Youn Lee, Ju Han Kim, Ji Hye Park   +4 more
openaire   +3 more sources