Results 251 to 260 of about 1,474,432 (298)

RNA sequencing in situ

Nature Biotechnology, 2014
Using a cell as an RNA sequencing chip enables spatial analyses of the transcriptome at subcellular resolution.
Paul, Ginart, Arjun, Raj
openaire   +2 more sources

RNA Sequencing

2011
This chapter describes the RNA sequencing (RNA-Seq) protocol, whereby RNA from yeast cells is prepared for sequencing on an Illumina Genome Analyzer. The protocol can easily be altered to use RNA from a different organism. This chapter covers RNA extraction, cDNA synthesis, cDNA fragmentation, and Illumina cDNA library generation and contains some ...
Karl, Waern, Ugrappa, Nagalakshmi, Michael, Snyder   +2 more
openaire   +2 more sources

RNA SEQUENCE ANALYSIS

Annual Review of Biochemistry, 1970
FRACTIONATION AND PURIFICATION OF RNA 228 Transfer RNA 228 Ribosomal RNA ... . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. 230 Viral RNA 230 SEPARATION AND ISOLATION OF POLYNUCLEOTIDES 231 Physical methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
openaire   +2 more sources

RNA sequencing

Anesthesiology, 2021
Eliana, Lucchinetti, Michael, Zaugg
  +5 more sources

Sequence-specific targeting of RNA

Methods, 2022
Post-transcriptional modifications play an important role in several processes, including translation, splicing, and RNA degradation in eukaryotic cells. To investigate the function of specific modifications it is of high interest to develop tools for sequence-specific RNA-targeting.
Lukas, Rösner, Dennis, Reichert, Kristina, Rau, Nils, Muthmann, Andrea, Rentmeister   +4 more
openaire   +2 more sources

Identification of Circular RNAs using RNA Sequencing

Journal of Visualized Experiments, 2019
Circular RNAs (circRNAs) are a class of non-coding RNAs involved in functions including micro-RNA (miRNA) regulation, mediation of protein-protein interactions, and regulation of parental gene transcription. In classical next generation RNA sequencing (RNA-seq), circRNAs are typically overlooked as a result of poly-A selection during construction of ...
Shobana, Sekar, Philipp, Geiger, Lori, Cuyugan, Annalee, Boyle, Geidy, Serrano, Thomas G, Beach, Winnie S, Liang   +6 more
openaire   +2 more sources

Sequence Design for RNA-RNA Interactions

The design of RNA sequences with desired structural properties presents a challenging computational problem with promising applications in biotechnology and biomedicine. Most regulatory RNAs function by forming RNA-RNA interactions, e.g., in order to regulate mRNA expression.
Maria Waldl, Hua-Ting Yao, Ivo L. Hofacker   +2 more
openaire   +2 more sources

A different approach to RNA sequencing

Nature, 1978
DURING the past few years techniques to simplify the systematic sequence determination of nucleic acids have become available; these involve the use of polyacrylamide gels from which the sequence may be directly and rapidly read off. The most recent of these, applicable to RNA fragments, rely on the base-specific nature of cleavage by T1 and U2 RNases1,
J, Stanley, S, Vassilenko
openaire   +2 more sources

RNA Sequencing

RNA-sequencing of wild-type and vimentin knockout THP-1 ...
  +4 more sources

Identification of Bacterial Small RNAs by RNA Sequencing

2014
Small regulatory RNAs (sRNAs) in bacteria are known to modulate gene expression and control a variety of processes including metabolic reactions, stress responses, and pathogenesis in response to environmental signals. A method to identify bacterial sRNAs on a genome-wide scale based on RNA sequencing (RNA-seq) is described that involves the ...
María, Gómez-Lozano, Rasmus Lykke, Marvig, Søren, Molin, Katherine S, Long   +3 more
openaire   +2 more sources