Results 151 to 160 of about 3,551,217 (248)

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Georgia's libertarian revolution / European Stability Initiative

, 2010
unknown
core   +1 more source

Low RNA stability signifies increased post-transcriptional regulation of cell identity genes. [PDF]

Nucleic Acids Res, 2023
Li Y, Yi Y, Lv J, Gao X, Yu Y, Babu SS, Bruno I, Zhao D, Xia B, Peng W, Zhu J, Chen H, Zhang L, Cao Q, Chen K.   +14 more
europepmc   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Buc Maintains Maternal RNA Stability and Embryogenesis in Zebrafish. [PDF]

Cells
Miao R, Xie Y, Han Q, Meng Y, Tang Q, Mei J, Ren F.   +6 more
europepmc   +1 more source

Role of the 5' end phosphorylation state for small RNA stability and target RNA regulation in bacteria. [PDF]

Nucleic Acids Res, 2023
Schilder A, Görke B.
europepmc   +1 more source

Small RNA pathways in mammalian oocytes

FEBS Open Bio, EarlyView.
Three distinct small RNA pathways operate in mammalian oocytes: RNAi interference (RNAi), the microRNA (miRNA) pathway, and the PIWI‐associated RNA (piRNA) pathway. These pathways use small RNAs to guide sequence‐specific repression and contribute to oocyte biology by targeting genes and mobile elements or appear insignificant since different ...
Petr Svoboda, Josef Pasulka
wiley   +1 more source

Improved Messenger RNA Stability and Biocompatibility Through Self-Gelatinizable Nucleic Acids. [PDF]

Biotechnol Bioeng
Tanifuji T, Kusamori K, Tanaka C, Sakai H, Itakura S, Nishikawa M.   +5 more
europepmc   +1 more source

Author Correction: RNA stability controlled by m⁶A methylation contributes to X-to-autosome dosage compensation in mammals. [PDF]

Nat Struct Mol Biol, 2023
Rücklé C, Körtel N, Basilicata MF, Busch A, Zhou Y, Hoch-Kraft P, Tretow K, Kielisch F, Bertin M, Pradhan M, Musheev M, Schweiger S, Niehrs C, Rausch O, Zarnack K, Keller Valsecchi CI, König J.   +16 more
europepmc   +1 more source

Transcripts enriched in codons that trigger P‐site tRNA‐mediated mRNA decay possess stable mRNA

FEBS Open Bio, EarlyView.
PTMD codons were first described by Mendel et al. as mediators of an mRNA decay pathway dependent on the human protein CNOT3, homologous to yeast Not5. Our findings confirm that PTMD codons destabilize transcripts; however, unlike in yeast, the human pathway specifically targets and slightly destabilizes primarily stable mRNAs.
Rodolfo Lopes Carneiro, Fernando Lucas Palhano   +1 more
wiley   +1 more source