Results 41 to 50 of about 511,041 (280)

The anti‐CRISPR protein AcrIE8.1 inhibits the type I‐E CRISPR‐Cas system by directly binding to the Cascade subunit Cas11

FEBS Letters, EarlyView.
In this study, we present the structure of AcrIE8.1, a previously uncharacterized anti‐CRISPR protein that inhibits the type I‐E CRISPR‐Cas system. Through a combination of structural and biochemical analyses, we demonstrate that AcrIE8.1 directly binds to the Cas11 subunit of the Cascade complex to inhibit the CRISPR‐Cas system.
Young Woo Kang, Hyun Ho Park
wiley   +1 more source

Long noncoding RNA BBOX1-AS1 increased radiotherapy sensitivity in colorectal cancer by stabilizing and activating PFK1

Translational Oncology, 2023
Purpose: Our study explored the effect of long noncoding RNA BBOX1-AS1 on colorectal cancer (CRC) radiosensitivity in vivo and in vitro. Methods: Differentially expressed lncRNAs in CRC were screened using a bioinformatics database and an online ...
Qi Wang, Xiao-Fei Li, Ying-Hui Zhou, Xiang-Hong Qin, Li-Hui Wang, Meng-Qing Xiao, Ke Cao, John K. Ma, Cheng-Hui Huang   +8 more
doaj   +1 more source

An intracellular transporter mitigates the CO2‐induced decline in iron content in Arabidopsis shoots

FEBS Letters, EarlyView.
This study identifies a gene encoding a transmembrane protein, MIC, which contributes to the reduction of shoot Fe content observed in plants under elevated CO2. MIC is a putative Fe transporter localized to the Golgi and endosomal compartments. Its post‐translational regulation in roots may represent a potential target for improving plant nutrition ...
Timothy Mozzanino, Meijie Li, Cécile Fizames, Mattia Adamo, Laurence Lejay, Christian Dubos, Matthieu Platre, Antoine Martin   +7 more
wiley   +1 more source

Kaposi's sarcoma-associated herpesvirus ORF57 protein binds and protects a nuclear noncoding RNA from cellular RNA decay pathways. [PDF]

PLoS Pathogens, 2010
The control of RNA stability is a key determinant in cellular gene expression. The stability of any transcript is modulated through the activity of cis- or trans-acting regulatory factors as well as cellular quality control systems that ensure the ...
Brooke B Sahin, Denish Patel, Nicholas K Conrad   +2 more
doaj   +1 more source

The Caenorhabditis elegans DPF‐3 and human DPP4 have tripeptidyl peptidase activity

FEBS Letters, EarlyView.
The dipeptidyl peptidase IV (DPPIV) family comprises serine proteases classically defined by their ability to remove dipeptides from the N‐termini of substrates, a feature that gave the family its name. Here, we report the discovery of a previously unrecognized tripeptidyl peptidase activity in DPPIV family members from two different species.
Aditya Trivedi, Rajani Kanth Gudipati
wiley   +1 more source

AMPK phosphosite profiling by label-free mass spectrometry reveals a multitude of mTORC1-regulated substrates

npj Metabolic Health and Disease
The nutrient-sensitive protein kinases AMPK and mTORC1 form a fundamental negative feedback loop that governs cell growth and proliferation. mTORC1 phosphorylates α2-S345 in the AMPK αβγ heterotrimer to suppress its activity and promote cell ...
William J. Smiles, Ashley J. Ovens, Dingyi Yu, Naomi X. Y. Ling, Andrea C. Poblete Goycoolea, Kaitlin R. Morrison, Emmanuel O. Murphy, Astrid Glaser, Sophie F. Monks O’Byrne, Scott Taylor, Alistair M. Chalk, Carl R. Walkley, Luke M. McAloon, John W. Scott, Bruce E. Kemp, Ashfaqul Hoque, Christopher G. Langendorf, Janni Petersen, Sandra Galic, Jonathan S. Oakhill   +19 more
doaj   +1 more source

Expanding access to HIV viral load testing: a systematic review of RNA stability in EDTA tubes and PPT beyond current time and temperature thresholds.

PLoS ONE, 2014
BackgroundHIV viral load (VL) testing is the gold standard for antiretroviral treatment monitoring, but many barriers exist to VL testing in resource-limited settings, including storage and transport limitations for whole blood and plasma.
Kimberly Bonner, Reed A Siemieniuk, Andrew Boozary, Teri Roberts, Emmanuel Fajardo, Jennifer Cohn   +5 more
doaj   +1 more source

Neuronal Cells Display Distinct Stability Controls of Alternative Polyadenylation mRNA Isoforms, Long Non-Coding RNAs, and Mitochondrial RNAs

Frontiers in Genetics, 2022
RNA stability plays an important role in gene expression. Here, using 3′ end sequencing of newly made and pre-existing poly(A)+ RNAs, we compare transcript stability in multiple human cell lines, including HEK293T, HepG2, and SH-SY5Y.
Aysegul Guvenek, Aysegul Guvenek, Jihae Shin, Lidia De Filippis, Dinghai Zheng, Wei Wang, Zhiping P. Pang, Bin Tian, Bin Tian   +8 more
doaj   +1 more source

Molecular bases of circadian magnesium rhythms across eukaryotes

FEBS Letters, EarlyView.
Circadian rhythms in intracellular [Mg2+] exist across eukaryotic kingdoms. Central roles for Mg2+ in metabolism suggest that Mg2+ rhythms could regulate daily cellular energy and metabolism. In this Perspective paper, we propose that ancestral prokaryotic transport proteins could be responsible for mediating Mg2+ rhythms and posit a feedback model ...
Helen K. Feord, Gerben van Ooijen
wiley   +1 more source

A Conserved Histophilus somni 23S Intervening Sequence Yields Functional, Fragmented 23S rRNA

Microbiology Spectrum, 2021
Histophilus somni is a Gram-negative bacterial organism that acts as an opportunistic pathogen and is a fastidious member of the Pasteurellaceae family associated with diseases of respiratory, reproductive, cardiac, and other tissues of ruminants.
Gregory P. Harhay, Dayna M. Harhay, Kerry D. Brader, Timothy P. L. Smith   +3 more
doaj   +1 more source