Results 81 to 90 of about 4,719,315 (357)
Stability of RNA virus attenuation approaches [PDF]
Vaccine, 2011 The greatest risk from live-attenuated vaccines is reversion to virulence. Particular concerns arise for RNA viruses, which exhibit high mutation frequencies. We examined the stability of 3 attenuation strategies for the alphavirus, Venezuelan equine encephalitis virus (VEEV): a traditional, point mutation-dependent attenuation approach exemplified by ...
Joan L. Kenney +6 more
openaire +3 more sources
Genome-wide analysis of lncRNA stability in human.
PLoS Computational Biology, 2021 Transcript stability is associated with many biological processes, and the factors affecting mRNA stability have been extensively studied. However, little is known about the features related to human long noncoding RNA (lncRNA) stability.
Kaiwen Shi +4 more
doaj +1 more source
FEBS Letters, EarlyView.This study reveals how prime editing guide RNA (pegRNA) secondary structure and reverse transcriptase template length affect prime editing efficiency in correcting the phospholamban R14del cardiomyopathy‐associated mutation. Insights support the design of structurally optimized enhanced pegRNAs for precise gene therapy.
Bing Yao +7 more
wiley +1 more source
Highly Mutable Linker Regions Regulate HIV-1 Rev Function and Stability. [PDF]
, 2019 HIV-1 Rev is an essential viral regulatory protein that facilitates the nuclear export of intron-containing viral mRNAs. It is organized into structured, functionally well-characterized motifs joined by less understood linker regions.
Fernandes, Jason D +4 more
core +3 more sources
Characterization of RNA hairpin loop stability [PDF]
Nucleic Acids Research, 1988 Fifteen RNA hairpins that share the same stem sequence and have homopolymer loops of A, C and U residues which vary in length from three to nine nucleotides were synthesized and their thermal stabilities determined. Tm varies as a function of loop size but is almost independent of loop composition.
Duncan R. Groebe, Olke C. Uhlenbeck
openaire +3 more sources
Molecular Plant-Microbe Interactions, 2004 Quantitative real-time polymerase chain reaction was used with specific TaqMan probes to examine transcription of selected hrp and effector genes in Pseudomonas syringae pv. phaseolicola strains 1448A (race 6) and 1449B (race 7).
R. Thwaites +4 more
doaj +1 more source
A Conserved Histophilus somni 23S Intervening Sequence Yields Functional, Fragmented 23S rRNA
Microbiology Spectrum, 2021 Histophilus somni is a Gram-negative bacterial organism that acts as an opportunistic pathogen and is a fastidious member of the Pasteurellaceae family associated with diseases of respiratory, reproductive, cardiac, and other tissues of ruminants.
Gregory P. Harhay +3 more
doaj +1 more source
FEBS Letters, EarlyView.In the adult T‐cell leukemia/lymphoma (ATL) cell line ED, the human T‐cell leukemia virus type 1 (HTLV‐1) provirus was integrated into the intron of the ift81 gene in the antisense orientation. Despite this integration, both the intact ift81 and the viral oncogene hbz were simultaneously expressed, likely due to the functional insufficiency of viral ...
Mayuko Yagi +5 more
wiley +1 more source
DNA and RNA stabilization v1 [PDF]
, 2020 DNA and RNA stabilization solution is an aqueous and non-toxic tissue storage reagent that rapidly permeates tissues to stabilize and protect nucleic acids in fresh specimens. Tissue pieces are harvested and immediately submerged in stabilization solution for storage.
openaire +2 more sources
Systematic Analysis of the Role of RNA-Binding Proteins in the Regulation of RNA Stability
PLoS Genetics, 2014 mRNA half-lives are transcript-specific and vary over a range of more than 100-fold in eukaryotic cells. mRNA stabilities can be regulated by sequence-specific RNA-binding proteins (RBPs), which bind to regulatory sequence elements and modulate the ...
A. Hasan +3 more
semanticscholar +1 more source