Results 71 to 80 of about 1,193,020 (266)

Phosphatidylcholine head group chemistry alters the extrahepatic accumulation of lipid-conjugated siRNA

Molecular Therapy: Nucleic Acids
Small interfering RNAs (siRNAs) are revolutionizing the treatment of liver-associated indications. Yet, robust delivery to extrahepatic tissues remains a challenge. Conjugating lipids (e.g., docosanoic acid [DCA]) to siRNA supports extrahepatic delivery,
Vignesh N. Hariharan, Takahiro Nakamura, Minwook Shin, Qi Tang, Vyankat Sontakke, Jillian Caiazzi, Samuel Hildebrand, Anastasia Khvorova, Ken Yamada   +8 more
doaj   +1 more source

CRISPR/Cas9-mediated genome editing induces exon skipping by alternative splicing or exon deletion

Genome Biology, 2017
CRISPR is widely used to disrupt gene function by inducing small insertions and deletions. Here, we show that some single-guide RNAs (sgRNAs) can induce exon skipping or large genomic deletions that delete exons. For example, CRISPR-mediated editing of β-
Haiwei Mou, Jordan L. Smith, Lingtao Peng, Hao Yin, Jill Moore, Xiao-Ou Zhang, Chun-Qing Song, Ankur Sheel, Qiongqiong Wu, Deniz M. Ozata, Yingxiang Li, Daniel G. Anderson, Charles P. Emerson, Erik J. Sontheimer, Melissa J. Moore, Zhiping Weng, Wen Xue   +16 more
doaj   +1 more source

Genome-wide detection of CRISPR editing in vivo using GUIDE-tag

Nature Communications, 2022
In vivo assessment of nuclease off-target activity has primarily been indirect or through ChIP-based detection of double-strand break DNA repair factors, which can be cumbersome.
Shun-Qing Liang, Pengpeng Liu, Jordan L. Smith, Esther Mintzer, Stacy Maitland, Xiaolong Dong, Qiyuan Yang, Jonathan Lee, Cole M. Haynes, Lihua Julie Zhu, Jonathan K. Watts, Erik J. Sontheimer, Scot A. Wolfe, Wen Xue   +13 more
doaj   +1 more source

Epigenetic reprogramming of lineage switching in cancer

FEBS Letters, EarlyView.
Cancer cells rarely commit to a single identity. Epigenetic mechanisms and tumor microenvironment cues push epithelial cells toward flexible, hybrid states that can shift into mesenchymal, neuroendocrine, or stem‐like fates, driving metastasis, drug resistance, and tumor heterogeneity. Targeting the epigenetic regulators behind these transitions, using
Ezgi Boyvatlı, Burcu Akman, Ezgi Bağırsakçı, Nese Atabey, Serap Erkek‐Ozhan   +4 more
wiley   +1 more source

Casein kinase II promotes piRNA production through direct phosphorylation of USTC component TOFU-4

Nature Communications
Piwi-interacting RNAs (piRNAs) are genomically encoded small RNAs that engage Piwi Argonaute proteins to direct mRNA surveillance and transposon silencing.
Gangming Zhang, Chunwei Zheng, Yue-he Ding, Craig Mello   +3 more
doaj   +1 more source

CubEd: a 3D printable fluorescence cube microscope optimized for classroom and teaching use.

Journal of Open Hardware
“When all you have is a hammer, everything is a nail” diametrically opposes the “right tool for the job” mentality. After observing graduate students in the biological sciences often adopting the former approach towards fluorescence light microscopy ...
Mathias Hammer, Jocelyn Tourtellotte, Colton Hormann, Selene Flemming, Brian Tran, Hui Rong, David Grunwald   +6 more
doaj   +1 more source

The microbiome in human skin aging

FEBS Letters, EarlyView.
Age‐related skin changes encompass the well‐known visible phenotypic alterations, together with microbiome dysbiosis and a series of molecular aging hallmarks. These hallmarks characterize not only a fully stablished aged phenotype but also the skin aging process itself.
Manuel Huerta Arana, Cornelia Wiegand, Omid Omrani, Mario Fabri   +3 more
wiley   +1 more source

Functions of RNAi Pathways in Ribosomal RNA Regulation

Argonaute proteins, guided by small RNAs, play crucial roles in gene regulation and genome protection through RNA interference (RNAi)-related mechanisms.
Shatskikh, Aleksei S, Klenov, Mikhail S, Fefelova, Elena A   +2 more
core   +1 more source

Partial depletion of plasminogen activator inhibitor‐1 decreases subcutaneous fat cell hypertrophy and liver cholesterol in high‐fat‐fed female mice

FEBS Letters, EarlyView.
Obesity raises blood levels of PAI‐1, a protein linked to metabolic dysfunction‐associated steatotic liver disease in people with obesity. In female mice fed a high‐fat diet, partially lowering PAI‐1 led to smaller subcutaneous fat cells and lower liver cholesterol, without changing body weight or insulin sensitivity.
Claudia E. Ramirez Bustamante, Robert Sharp, Pradip K. Saha, Elizabeth S. Anaya, Elizabeth L. Lieu, Evelyn L. de Groot, Pooja Patel, Mi Jeong Heo, Aaron R. Cox, Kang Ho Kim, Sean M. Hartig   +10 more
wiley   +1 more source

Exploring the Emergence of RNA Nucleosides and Nucleotides on the Early Earth [PDF]

, 2018
Understanding how life began is one of the most fascinating problems to solve. By approaching this enigma from a chemistry perspective, the goal is to define what series of chemical reactions could lead to the synthesis of nucleotides, amino acids ...
Biscans, Annabelle
core   +1 more source