Results 111 to 120 of about 813,434 (316)

Kissing interaction between 3' noncoding and coding sequences is essential for porcine arterivirus RNA replication

, 2002
We used an infectious cDNA clone of Porcine reproductive and respiratory syndrome virus (PRRSV) to investigate the presence of essential replication elements in the region of the genome encoding the structural proteins.
R. C. L. Olsthoorn, Rottier, P.J.M., J. J. M. Meulenberg, Olsthoorn, R.R.C.L., M. H. Verheije, P. J. M. Rottier, M. V. Kroese, Meulenberg, J.J.M., Verheije, M.H., Olsthoorn, R.C.L., Kroese, M.V.   +10 more
core   +1 more source

YIPFα1A expression is regulated by multilayered molecular mechanisms

FEBS Open Bio, EarlyView.
YIPFα1A, a five‐pass Golgi protein, is regulated at multiple layers. (1) Rare‐codon enrichment drives translation‐coupled mRNA decay. (2) A proximal 3′‐UTR element stabilizes mRNA. (3) A distal 3′‐UTR element included by alternate poly(A) site usage represses translation, which can be overridden by the proximal 3′‐UTR element.
Tokio Takaji, Yurika Nakanishi, Nobuhiro Nakamura   +2 more
wiley   +1 more source

Functional domains of the influenza A virus PB2 protein:identification of NP- and PB1-binding sites

, 2004
Influenza virus genomic RNA segments are packaged into ribonucleoprotein (RNP) structures by the PB1, PB2, and PA subunits of an RNA polymerase and a single-strand RNA-binding nucleoprotein (NP).
Poole, Emma, Digard, Paul, Medcalf, Liz, Elton, Debra, Poole, Emma L   +4 more
core   +1 more source

Identifying transcription factors controlling the basal expression of human MRP4 highlights a substantial role for Sp1

FEBS Open Bio, EarlyView.
The MRP4 transporter exports several drugs and signaling molecules. Here, we identified key promoter elements regulating basal MRP4 expression. Using reporter assays, we defined a conserved region with essential Sp1 and contributory Ets sites, which controlled basal MRP4 expression.
Debora Singer, Angelique Kragl, Katrin Ziems, Juliane Glaubitz, Sophie Grammbauer, Susanne Hildebrandt, Mladen V. Tzvetkov, Gabriele Jedlitschky   +7 more
wiley   +1 more source

RNA-protein interaction prediction without high-throughput data: An overview and benchmark of in silico tools

Computational and Structural Biotechnology Journal
RNA-protein interactions (RPIs) are crucial for accurately operating various processes in and between organisms across kingdoms of life. Mutual detection of RPI partner molecules depends on distinct sequential, structural, or thermodynamic features ...
Sarah Krautwurst, Kevin Lamkiewicz
doaj   +1 more source

Bluetongue virus non-structural protein 1 is a positive regulator of viral protein synthesis. [PDF]

, 2012
BACKGROUND: Bluetongue virus (BTV) is a double-stranded RNA (dsRNA) virus of the Reoviridae family, which encodes its genes in ten linear dsRNA segments.
Roy Polly, Boyce, Mark, Roy, Polly, Celma, Cristina CP, Cristina C P Celma, Boyce Mark, Mark Boyce, Polly Roy, Celma Cristina C P   +8 more
core   +1 more source

Derivation and characterization of retinal pigment epithelium from urine‐derived iPSCs

FEBS Open Bio, EarlyView.
Age‐related macular degeneration causes vision loss via RPE dysfunction and loss. Traditional iPSC therapies rely on invasive biopsies, limiting scalability. Here, we utilize urine‐derived stem cells as an accessible source to generate u‐iPSCs, successfully differentiated into pigmented RPE. This “Urine‐to‐Retina” platform provides a promising path for
Daniella Beiner, Hainan Zhu, Carol Christine Bosholm, Heuy‐Ching Wang, Tracy Criswell, Anthony Atala, Jian‐Xing Ma, Yuanyuan Zhang   +7 more
wiley   +1 more source

Genome-scale CRISPR-Cas9 screen identifies host factors as potential therapeutic targets for SARS-CoV-2 infection

iScience
Summary: Although many host factors important for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection have been reported, the mechanisms by which the virus interacts with host cells remain elusive.
Madoka Sakai, Yoshie Masuda, Yusuke Tarumoto, Naoyuki Aihara, Yugo Tsunoda, Michiko Iwata, Yumiko Kamiya, Ryo Komorizono, Takeshi Noda, Kosuke Yusa, Keizo Tomonaga, Akiko Makino   +11 more
doaj   +1 more source

Activation of the interferon induction cascade by influenza A viruses requires viral RNA synthesis and nuclear export

, 2014
This work is supported by grants from the Wellcome Trust (grants 087751/A/08/Z) and MRC (G1001726/1).We have examined the requirements for virus transcription and replication and thus the roles of input and progeny genomes in the generation of interferon
Randall, Richard E, Smith, Matt, Killip, Marian J, Jackson, David   +3 more
core   +1 more source

Optineurin negatively regulates the induction of IFNβ in response to RNA virus infection [PDF]

, 2010
The innate immune response provides a critical defense against microbial infections, including viruses. These are recognised by pattern recognition receptors including Toll-like receptors (TLRs) and RIG-I like helicases (RLHs).
Harris, M., Macdonald, A., Kohl, A., Richards, KH, Wetherill, L, Kohl, Alain, MacDonald, A, Mankouri, Jamel, Kohl, A, Elliott, Richard M., Elliott, RM, Alain Kohl, Wetherill, L.F., Rennos Fragkoudis, Harris Mark, Andrew Macdonald, Richards Kathryn H., Richards, K.H., Richards, K, Laura F Wetherill, Mankouri, J., Richards, Kathryn H., Mankouri, J, Jamel Mankouri, Elliott, R.M., Wetherill Laura F., Fragkoudis, R., Richard M Elliott, Macdonald, Andrew, Harris, M, Wetherill, Laura F., Kohl Alain, Elliott Richard M., Macdonald Andrew, Wetherill, LF, Elliott, Richard Michael, Mark Harris, Harris, Mark, Fragkoudis, R, Kathryn H Richards, Fragkoudis, Rennos, Mankouri Jamel, Fragkoudis Rennos   +42 more
core   +1 more source