Results 191 to 200 of about 14,336,830 (357)

Transcript Identification Using Arrayed Hydrogels With TrapFISH

Advanced Materials Technologies, EarlyView.
TrapFISH is a novel method for high‐throughput single‐cell transcriptomics. It embeds cells in hyaluronic acid hydrogel beads and uses probe hybridization to quantify custom gene panels. By arraying hydrogels in microfluidic devices, cell barcoding is removed, enhancing scalability for studying cellular heterogeneity and rare cell states by targeting ...
David B. Morse, Zenon Toprakcioglu, Akhila Denduluri‐Marthi, James D. Brenton, Craig J. Thomas, Tuomas P. J. Knowles   +5 more
wiley   +1 more source

Expression of an active form of recombinant Ty1 reverse transcriptase in Escherichia coli: a fusion protein containing the C-terminal region of the Ty1 integrase linked to the reverse transcriptase‒RNase H domain exhibits polymerase and RNase H activities [PDF]

, 2000
M.L. Wilhelm, Mansour Boutabout, François-Xavier WILHELM   +2 more
openalex   +2 more sources

Correction: Ultrasensitive fluorescent aptasensor for CRP detection based on the RNase H assisted DNA recycling signal amplification strategy. [PDF]

RSC Adv, 2022
Liu Z, Luo D, Ren F, Ran F, Chen W, Zhang B, Wang C, Chen H, Wei J, Chen Q.   +9 more
europepmc   +1 more source

Nanomaterial‐Based Optical Biosensors for SARS‐CoV‐2 Detection: A Retrospective of the Pandemic

Advanced Sensor Research, EarlyView.
This review discusses nanomaterial‐based optical biosensors developed for or adapted to the detection of severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2). It concludes by providing a perspective on how lessons learned during the coronavirus disease of 2019 (COVID‐19) pandemic may be applied for future research on nanomaterial‐based ...
Flavie Martin, Scott G. Harroun, Michel Meunier   +2 more
wiley   +1 more source

Endogenous reverse transcriptase and RNase H-mediated antiviral mechanism in embryonic stem cells. [PDF]

Cell Res, 2021
Wu J, Wu C, Xing F, Cao L, Zeng W, Guo L, Li P, Zhong Y, Jiang H, Luo M, Shi G, Bu L, Ji Y, Hou P, Peng H, Huang J, Li C, Guo D.   +17 more
europepmc   +1 more source

Residues in the αH and αI Helices of the HIV-1 Reverse Transcriptase Thumb Subdomain Required for the Specificity of RNase H-catalyzed Removal of the Polypurine Tract Primer [PDF]

, 1999
Michael D. Powell, William A. Beard, Katarzyna Bębenek, Kathryn J. Howard, Stuart F.J. Le Grice, Thomas A. Darden, Thomas A. Kunkel, Samuel H. Wilson, Judith G. Levin   +8 more
openalex   +1 more source

Genetic Engineering Methods in Primary T Cells

Advanced Therapeutics, EarlyView.
Primary T cells can be engineered to confer them with novel therapeutic functions, allowing them to treat a variety of conditions. Genetic engineering can be either stable or transient, aiming to either express or inhibit a target gene. This review discusses the various genetic engineering tools available as well as their characteristics and ...
Anthony Youssef, Hui‐Shan Li
wiley   +1 more source

Natural Occurring Polymorphisms in HIV-1 Integrase and RNase H Regulate Viral Release and Autoprocessing. [PDF]

J Virol, 2021
Imamichi T, Bernbaum JG, Laverdure S, Yang J, Chen Q, Highbarger H, Hao M, Sui H, Dewar R, Chang W, Lane HC.   +10 more
europepmc   +1 more source

R-ChIP Using Inactive RNase H Reveals Dynamic Coupling of R-loops with Transcriptional Pausing at Gene Promoters.

Molecules and Cells, 2017
Liang Chen, Jia-Yu Chen, Xuan Zhang, Y. Gu, Rui Xiao, C. Shao, Peng Tang, Hao Qian, Daji Luo, Hai-Ri Li, Yu Zhou, Dong-er Zhang, Xiang-Dong Fu   +12 more
semanticscholar   +1 more source

Identification of two different RNase H activities associated with yeast RNA polymerase A.

, 1979
F. Iborra, Janine Huet, B. Bréant, André Sentenac, P. Fromageot   +4 more
openalex   +1 more source