Results 251 to 260 of about 290,253 (274)

RNase H Activation by Stereoregular Boranophosphate Oligonucleotide

ChemInform, 2003
AbstractFor Abstract see ChemInform Abstract in Full Text.
Xin, Wang, Mikhail, Dobrikov, Dmitri, Sergueev, Barbara Ramsay, Shaw   +3 more
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Function of RNase H in DNA replication revealed by RNase H defective mutants of Escherichia coli

Molecular and General Genetics MGG, 1984
Escherichia coli rnh mutants were isolated using localized mutagenesis and selective measurements of RNase H activity in mutagenized cell extracts with [3H]poly(rC) X poly(dG) as substrate. RNase H activity in extracts of one mutant, ON152 (rnh-91), was undetectable (less than 0.05% of that of wild-type cells).
T, Ogawa, T, Okazaki
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Mammalian retrovirus-associated RNase H is virus coded

Journal of Virology, 1978
RNase H of a temperature-sensitive mutant of Rauscher murine leukemia virus is thermolabile, establishing this activity as a virus-coded function of the mammalian type C virus reverse transcriptase.
M H, Lai, I M, Verma, S R, Tronick, S A, Aaronson   +3 more
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Boranophosphates Support the RNase H Cleavage of Polyribonucleotides

Antisense and Nucleic Acid Drug Development, 1999
Modification of the phosphodiester linkages in DNA by replacing one of the nonbridging oxygens with borane, BH3, produces an isoelectronic mimic of DNA called boranophosphates. Nonstereoregular oligodeoxyribonucleoside all-boranophosphates are shown here for the first time to elicit the RNase H hydrolysis of polyribonucleotides. We compared the ability
V K, Rait, B R, Shaw
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An RNase H-powered DNA walking machine for sensitive detection of RNase H and the screening of related inhibitors

Nanoscale, 2020
A signal amplification strategy based on an RNase H-powered DNA walking machine for RNase H activity detection.
Yafang Wang, Namin Hu, Chang Liu, Cunpeng Nie, Manman He, Juan Zhang, Qiaoqin Yu, Chuan Zhao, Tingting Chen, Xia Chu   +9 more
openaire   +2 more sources

Improvement of Reverse Transcription PCR by RNase H

Bioscience, Biotechnology, and Biochemistry, 2003
An improvement in the method of the Reverse Transcription PCR (RT-PCR) using RNase H is proposed here. We succeeded in RT-PCR amplification against the full sequence of the coding region (8.9 kb) of the Insulin-like growth factor II receptor gene which has the area called the GC-block of about 90% GC contents at the 5' terminal.
Masao, Kitabayashi, Muneharu, Esaka
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Chemically modified oligonucleotides with efficient RNase H response

Bioorganic & Medicinal Chemistry Letters, 2008
Ten different chemically modified nucleosides were incorporated into short DNA strands (chimeric oligonucleotides ON3-ON12 and ON15-ON24) and then tested for their capacity to mediate RNAse H cleavage of the complementary RNA strand. The modifications were placed at two central positions directly in the RNase H cleaving region.
Vester, Birte, Boel, Anne Marie, Lobedanz, Sune, Babu, B Ravindra, Raunkjaer, Michael, Lindegaard, Dorthe, Raunak, , Hrdlicka, Patrick J, Højland, Torben, Sharma, Pawan K, Kumar, Surender, Nielsen, Poul, Wengel, Jesper   +12 more
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Reverse transcriptase-associated RNase H. Part IV. Pyrophosphate does not inhibit RNase H activity of AMV DNA polymerase

Biochemical and Biophysical Research Communications, 1979
Abstract Sodium pyrophosphate, a known inhibitor of DNA polymerases, strongly inhibits DNA synthesis directed by various synthetic and natural template-primers and catalysed by reverse transcriptases from AMV and RLV, but has no effect on the reverse transcriptase-associated ribonuclease H activity.
A, Srivastava, M J, Modak
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RNase H-defective mutants of Escherichia coli: A possible discriminatory role of RNase H in initiation of DNA replication

Molecular and General Genetics MGG, 1984
Mutants of Escherichia coli completely deficient in RNase H activity were isolated by inserting transposon Tn3 into the structural gene for RNase H, rnh, and its promoter. These rnh- mutants exhibited the following phenotypes; (1) the mutants grew fairly normally, (2) rnh- cells could be transformed with ColE1 derivative plasmids, pBR322 and pML21 ...
T, Horiuchi, H, Maki, M, Sekiguchi
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Selective mRNA degradation by antisense oligonucleotide-2,5A chimeras: Involvement of RNase H and RNase L

Biochimie, 1998
Antisense oligonucleotides (ON) allow the specific control of gene expression and phosphorothioate derivatives are currently being evaluated for possible clinical applications. Numerous second generation ON analogues with improved pharmacological properties have been described.
I, Robbins, G, Mitta, S, Vichier-Guerre, R, Sobol, A, Ubysz, B, Rayner, B, Lebleu   +6 more
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