Results 281 to 290 of about 476,060 (306)

An RNase H-powered DNA walking machine for sensitive detection of RNase H and the screening of related inhibitors

Nanoscale, 2020
A signal amplification strategy based on an RNase H-powered DNA walking machine for RNase H activity detection.
Yafang Wang, Namin Hu, Chang Liu, Cunpeng Nie, Manman He, Juan Zhang, Qiaoqin Yu, Chuan Zhao, Tingting Chen, Xia Chu   +9 more
openaire   +3 more sources

RNases H: Structure and mechanism

DNA Repair, 2019
RNases H are a family of endonucleases that hydrolyze RNA residues in various nucleic acids. These enzymes are present in all branches of life, and their counterpart domains are also found in reverse transcriptases (RTs) from retroviruses and retroelements.
Marcin Nowotny, Malwina Hyjek, Małgorzata Figiel   +2 more
openaire   +3 more sources

Function of RNase H in DNA replication revealed by RNase H defective mutants of Escherichia coli

Molecular and General Genetics MGG, 1984
Escherichia coli rnh mutants were isolated using localized mutagenesis and selective measurements of RNase H activity in mutagenized cell extracts with [3H]poly(rC) X poly(dG) as substrate. RNase H activity in extracts of one mutant, ON152 (rnh-91), was undetectable (less than 0.05% of that of wild-type cells).
Tuneko Okazaki, Tohru Ogawa
openaire   +3 more sources

Stereochemical Course of Escherichia coli RNase H.

ChemInform, 2002
A new enzymatic method has allowed the assignment of the stereochemistry of E. coli RNase-H-assisted hydrolysis of RNA labelled within the scissile bond with (R(p))-phosphorothioate. This method is based on a stereospecific, two-step enzymatic conversion of cytidine 5'-[(18)O]phosphorothioate into the corresponding 5'-alpha-[(18)O]thiotriphosphate ...
Alina Owczarek, Maria Koziołkiewicz, Wojciech J. Stec, Agnieszka Krakowiak   +3 more
openaire   +4 more sources

RNase H Activation by Stereoregular Boranophosphate Oligonucleotide

ChemInform, 2003
AbstractFor Abstract see ChemInform Abstract in Full Text.
Dmitri S. Sergueev, Barbara Ramsay Shaw, Xin Wang, Mikhail I. Dobrikov   +3 more
openaire   +4 more sources

Pyridopyrimidinone inhibitors of HIV-1 RNase H

European Journal of Medicinal Chemistry, 2014
Using a structure based pharmacophore design, a weak inhibitor of RNase H, identified from a small library of two metal binding HIV-1 integrase inhibitors, was optimized for potency and physicochemical properties. This manuscript describes the SAR and in vivo DMPK for the pyridopyrimidinone class of inhibitors.
Emile J. Velthuisen, Brian A. Johns, Peter Gerondelis, Yan Chen, Ming Li, Ke Mou, Wenwen Zhang, John W. Seal, Kendra E. Hightower, Sonia R. Miranda, Kevin Brown, Lisa Leesnitzer   +11 more
openaire   +3 more sources

The RNase H Mechanism

2007
Antisense oligonucleotides (ASOs) are designed to modulate the information transfer from gene to protein-in essence to alter mRNA intermediary metabolism. mRNA intermediary metabolism is extremely complex beginning with transcription and concluding with degradation usually after translation.
Hongjiang Wu, Stanley T. Crooke, Walt F. Lima   +2 more
openaire   +2 more sources

A radar-like DNA monitor for RNase H-targeted natural compounds screening and RNase H activity in situ detection

The Analyst, 2021
Radar-like monitor for RNase H detection in vitro and for intercellular imaging.
Yalei Hu, Qian Xie, Li Chang, Xueqing Tao, Chunyi Tong, Bin Liu, Wei Wang   +6 more
openaire   +3 more sources

Discrimination between mammalian RNases H-1 and H-2

Analytical Biochemistry, 1991
The two principal RNases H in mammalian cells, H-1 and H-2, differ in their responses to sale, divalent metal, and sulfhydryl inhibition. Specific reaction conditions that provide unambiguous discrimination between RNases H-1 and H-2 with only two assays are described. The assays were used for identification in a new purification procedure for RNases H-
Cheryl J. Heard, Mehran Goulian
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Boranophosphates Support the RNase H Cleavage of Polyribonucleotides

Antisense and Nucleic Acid Drug Development, 1999
Modification of the phosphodiester linkages in DNA by replacing one of the nonbridging oxygens with borane, BH3, produces an isoelectronic mimic of DNA called boranophosphates. Nonstereoregular oligodeoxyribonucleoside all-boranophosphates are shown here for the first time to elicit the RNase H hydrolysis of polyribonucleotides. We compared the ability
Vladimir Rait, Barbara Ramsay Shaw
openaire   +2 more sources