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[68] Preparation of bacterial DNA by the phenol-pH 9-RNases method
, 1967 Publisher Summary This chapter describes the method for the extraction of DNA from bacterial cells using phenol. Phenol treatment is considered one of the most effective methods for deproteinization. Whereas a neutral or acidic buffer (pH 5-7) extracts RNA into aqueous phase, rather than DNA from bacterial cells, in phenol treatment slightly alkaline
Kin‐ichiro Miura
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Crystal Structure of the phosphorolytic exoribonuclease RNase PH from Bacillus subtilis
, 2004 Lene S. Harlow +3 more
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Crystal Structure Of The tRNA Processing Enzyme Rnase pH From Pseudomonas Aeruginosa
, 2004 Suk-Jung Choi +4 more
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ACS Applied Materials and Interfaces, 2021
Enrichment and detection of glycopeptides are an important clinical measure for the diagnosis of complex diseases. Enrichment materials play a key role in this process; they must have an effective sample-screening ability to eliminate the interference of
Chun Zhang +7 more
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Enrichment and detection of glycopeptides are an important clinical measure for the diagnosis of complex diseases. Enrichment materials play a key role in this process; they must have an effective sample-screening ability to eliminate the interference of
Chun Zhang +7 more
semanticscholar +1 more source
Journal of the American Chemical Society, 1994
J. Straub, C. Lim, M. Karplus
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J. Straub, C. Lim, M. Karplus
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Journal of the Science of Food and Agriculture, 1978 AbstractAutolytic breakdown of the RNA of Saccharomyces cerevisiae can be induced by subjecting a suspension of yeast in NaCl solution at pH 6 to heat shock, produced by bringing the suspension to 50°C and then adding enough water at 100°C to increase the temperature momentarily to 68°C, followed after 1 min by the addition of cold water to reduce it ...
William E. Trevelyan
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Atomic resolution structures of rnase A at six ph values
2002The diffraction pattern of protein crystals extending to atomic resolution guarantees a very accurate picture of the molecular structure and enables the study of subtle phenomena related to protein functionality. Six structures of bovine pancreatic ribonuclease at the pH* values 5.2, 5.9, 6.3, 7.1, 8.0 and 8.8 and at resolution limits in the range 1.05-
SICA, FILOMENA +5 more
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Molecular Pharmaceutics, 2017
The specific transport of bioactive proteins into designated target cells is an interesting and challenging perspective for the generation of innovative biopharmaceuticals. Natural protein cytotoxins perform this task with outstanding efficacy.
Xiaowen Liu +5 more
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The specific transport of bioactive proteins into designated target cells is an interesting and challenging perspective for the generation of innovative biopharmaceuticals. Natural protein cytotoxins perform this task with outstanding efficacy.
Xiaowen Liu +5 more
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Advanced Healthcare Materials
Rheumatoid arthritis (RA) is a chronic immune disease characterized by the infiltration of immune cells and the proliferation of fibroblast‐like synoviocytes (FLS) at the joint site, leading to inflammation and joint destruction.
Saisai Ge +12 more
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Rheumatoid arthritis (RA) is a chronic immune disease characterized by the infiltration of immune cells and the proliferation of fibroblast‐like synoviocytes (FLS) at the joint site, leading to inflammation and joint destruction.
Saisai Ge +12 more
semanticscholar +1 more source