Overexpression and rapid purification of the orfE/rph gene product, RNase PH of Escherichia coli.
Journal of Biological Chemistry, 1992 The pyrE gene, encoding the pyrimidine biosynthetic enzyme orotate phosphoribosyltransferase, is the promoter distal gene of the dicistronic orfE-pyrE operon. The promoter proximal orfE gene, whose transcription and translation is important for regulation of the pyrE attenuator, encodes a 238-amino acid residue protein which was recently identified as ...
K.F. Jensen +2 more
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USPPAR is a cost-effective, scalable, and highly sensitive single-cell RNA sequencing workflow compatible with diverse specimens. [PDF]
PLoS BiologySingle-cell RNA sequencing (scRNA-seq) requires high sensitivity, throughput, and broad compatibility across specimens. Current high-capacity methods lack sensitivity compared to low-capacity counterparts. Moreover, tissue-specific methods for collecting
Ya-Wen Hsueh, Po-Min Chiang
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Protein–Protein Interactions between Human Exosome Components Support the Assembly of RNase PH-type Subunits into a Six-membered PNPase-like Ring [PDF]
Journal of Molecular Biology, 2002 The exosome is a complex of 3'-->5' exoribonucleases, which functions in a variety of cellular processes, all requiring the processing or degradation of RNA. Here we present a model for the assembly of the six human RNase PH-like exosome subunits into a hexameric ring structure.
Reinout Raijmakers +3 more
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Crystal structure of a bacterial tRNA-precursor processing enzyme: RNase PH fromBacillus subtilis [PDF]
Acta Crystallographica Section A Foundations of Crystallography, 2002 Anders Kadziola +3 more
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RNase 7 contributes to the cutaneous defense against Enterococcus faecium. [PDF]
PLoS ONE, 2009 BackgroundHuman skin is able to mount a fast response against invading microorganisms by the release of antimicrobial proteins such as the ribonuclease RNase 7. Because RNase 7 exhibits high activity against Enterococcus faecium the aim of this study was
Bente Köten +5 more
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mBio, 2022 The Bacillus subtilis genome encodes four 3′ exoribonucleases: polynucleotide phosphorylase (PNPase), RNase R, RNase PH, and YhaM. Previous work showed that PNPase, encoded by the pnpA gene, is the major 3′ exonuclease involved in mRNA turnover; in a ...
Shivani Chhabra +4 more
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Phylogenetic analyses and characterization of RNase X25 from Drosophila melanogaster suggest a conserved housekeeping role and additional functions for RNase T2 enzymes in protostomes. [PDF]
PLoS ONE, 2014 Ribonucleases belonging to the RNase T2 family are enzymes associated with the secretory pathway that are almost absolutely conserved in all eukaryotes.
Linda Ambrosio +5 more
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Biomimetic selenocystine based dynamic combinatorial chemistry for thiol-disulfide exchange
Nature Communications, 2021 Thiol-disulfide exchange is an extensively used reversible reaction in dynamic combinatorial chemistry, but usually requires long time to reach equilibrium.
Andrea Canal-Martín +1 more
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Journal of Experimental Nanoscience, 2023 In this study, we have designed an anticancer drug delivery framework by a one-pot technique using a zeolitic imidazolate framework (ZIF) as the carrier. The chitosan-coated Aminopterin (AMT) loaded with zeolitic imidazolate framework (ZIF-90) CS@AMT@ZIF-
Limin Zhu, Shuangyan Wang
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Multiple Patterns of Regulation and Overexpression of a Ribonuclease-Like Pathogenesis-Related Protein Gene, OsPR10a, Conferring Disease Resistance in Rice and Arabidopsis. [PDF]
PLoS ONE, 2016 An abundant 17 kDa RNase, encoded by OsPR10a (also known as PBZ1), was purified from Pi-starved rice suspension-cultured cells. Biochemical analysis showed that the range of optimal temperature for its RNase activity was 40-70°C and the optimum pH was 5 ...
Li-Fen Huang +9 more
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