Duplex DNA from Sites of Helicase-Polymerase Uncoupling Links Non-B DNA Structure Formation to Replicative Stress [PDF]
, 2020 BACKGROUND: Replication impediments can produce helicase-polymerase uncoupling allowing lagging strand synthesis to continue for as much as 6 kb from the site of the impediment.
Abdelhamid, Mahmoud A S +9 more
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Dependence of ethanol effects on protein charges [PDF]
, 2014 Ethanol is used as a conventional disinfectant solution. It is highly effective against enveloped viruses due to its effects on virus membranes. It also confers inactivation of non-enveloped viruses, which can be ascribed to conformational changes or ...
Arakawa Tsutomu +3 more
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Refolding of ribonuclease A in vitro and conformational characterization by spectrofluorimetry
浙江大学学报. 农业与生命科学版, 2006 Ribonuclease A (RNase A) was employed as a model protein to understand the formation of correct three-dimensional structure in the refolding of recombinant proteins in vitro.
TAN Pei-sheng +4 more
doaj +1 more source
Effects of rnase iii rncs gene deletion on stress response, biofilm formation and virulence of listeria monocytogenes [PDF]
Kafkas Universitesi Veteriner Fakültesi Dergisi, 2019 The ribonuclease III (RNase III) is an important enzyme system that regulates non-coding RNA (ncRNA) levels. In this study, LM-ΔrncS gene deletion strain was investigated by gene overlap extension PCR (SOE-PCR) and homologous recombination techniques ...
Lixia WANG +9 more
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The structure of Rph, an exoribonuclease from Bacillus anthracis, at 1.7 angstrom resolution [PDF]
, 2008 Maturation of tRNA precursors into functional tRNA molecules requires trimming of the primary transcript at both the 5' and 3' ends. Cleavage of nucleotides from the 3' stem of tRNA precursors, releasing nucleotide diphosphates, is accomplished in ...
Blagova, Elena V. +5 more
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A multifunctional ribonuclease A-conjugated carbon dot cluster nanosystem for synchronous cancer imaging and therapy [PDF]
, 2014 Carbon dots exhibit great potential in applications such as molecular imaging and in vivo molecular tracking. However, how to enhance fluorescence intensity of carbon dots has become a great challenge.
Chao Li +7 more
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Frontiers in Microbiology, 2020 MSMEG_4305 is a two-domain protein of Mycolicibacterium smegmatis (Mycobacterium smegmatis) (Mycolicibacterium smegmatis). The N-terminal domain of MSMEG_4305 encodes an RNase H type I.
Bożena Czubat +7 more
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Results of PCR diagnostics after gingiva soft tissue augmentation
Эндодонтия Today, 2022 Aim. To demonstrate the results of polymerase chain reaction in real time.Materials and methods. The gum fragment was placed in 1 ml of RNA-leiter ("QIAGEN", Germany), incubated for a day at +4C, samples were stored at -70 °C.To obtain cDNA from the RNA ...
A. B. Adzhieva +4 more
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A general approach to high-yield biosynthesis of chimeric RNAs bearing various types of functional small RNAs for broad applications. [PDF]
, 2015 RNA research and therapy relies primarily on synthetic RNAs. We employed recombinant RNA technology toward large-scale production of pre-miRNA agents in bacteria, but found the majority of target RNAs were not or negligibly expressed. We thus developed a
Chen, Qiu-Xia +4 more
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A functional RNase P protein subunit of bacterial origin in some eukaryotes [PDF]
, 2011 RNase P catalyzes 5′-maturation of tRNAs. While bacterial RNase P comprises an RNA catalyst and a protein cofactor, the eukaryotic (nuclear) variant contains an RNA and up to ten proteins, all unrelated to the bacterial protein.
Bernal Bayard, Pilar +5 more
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