The correlation of RNase A enzymatic activity with the changes in the distance between Nepsilon2-His12 and N delta1-His119 upon addition of stabilizing and destabilizing salts. [PDF]
The effect of stabilizing and destabilizing salts on the catalytic behavior of ribonuclease A (RNase A) was investigated at pH 7.5 and 25 degrees C, using spectrophotometric, viscometric and molecular dynamic methods.
A. A. Moosavi-Movahedi +50 more
core +1 more source
Basofilia e anisotropia nucleares em células de camundongo após tratamento com oxamniquine
Revista do Instituto de Medicina Tropical de São Paulo, 1984 Com o objetivo de se estudar a ação da oxamniquine, uma droga utilizada no tratamento da esquistossomose, sobre a cromatina de núcleos de células animais, foram estudados os padrões de basofilia e anisotropia nucleares em hepatócitos, em células do ...
Ivanira José Bechara +1 more
doaj +1 more source
Histone Mutants Separate R Loop Formation from Genome Instability Induction [PDF]
, 2017 R loops have positive physiological roles, but they can also be deleterious by causing genome instability, and the mechanisms for this are unknown. Here we identified yeast histone H3 and H4 mutations that facilitate R loops but do not cause instability.
Aguilera López, Andrés +5 more
core +1 more source
Elongation Factor TFIIS Prevents Transcription Stress and R-Loop Accumulation to Maintain Genome Stability [PDF]
, 2019 Although correlations between RNA polymerase II (RNAPII) transcription stress, R-loops, and genome instability have been established, the mechanisms underlying these connections remain poorly understood.
Aguilera López, Andrés +10 more
core +2 more sources
FEBS Letters, EarlyView.This study reveals how prime editing guide RNA (pegRNA) secondary structure and reverse transcriptase template length affect prime editing efficiency in correcting the phospholamban R14del cardiomyopathy‐associated mutation. Insights support the design of structurally optimized enhanced pegRNAs for precise gene therapy.
Bing Yao +7 more
wiley +1 more source
Production and characterization of bacteriocin produced by lactic acid bacteria isolated from rusip
Squalen, 2013 Research was conducted to produce and characterize bacteriocin produced by lactic acid bacteria (LAB) isolated from rusip, a traditional Bangkanese fermented fish product.
Arifah Kusumarwati +2 more
doaj +1 more source
A general approach to high-yield biosynthesis of chimeric RNAs bearing various types of functional small RNAs for broad applications. [PDF]
, 2015 RNA research and therapy relies primarily on synthetic RNAs. We employed recombinant RNA technology toward large-scale production of pre-miRNA agents in bacteria, but found the majority of target RNAs were not or negligibly expressed. We thus developed a
Chen, Qiu-Xia +4 more
core +2 more sources
Transcriptome‐wide analysis of circRNA and RBP profiles and their molecular relevance for GBM
Molecular Oncology, EarlyView.CircRNAs are differentially expressed in glioblastoma primary tumors and might serve as therapeutic targets and diagnostic markers. The investigation of circRNA and RNA‐binding proteins (RBPs) interactions shows that distinct RBPs play a role in circRNA biogenesis and function.
Julia Latowska‐Łysiak +14 more
wiley +1 more source
RNA is an integral component of chromatin that contributes to its structural organization.
PLoS ONE, 2007 Chromatin structure is influenced by multiples factors, such as pH, temperature, nature and concentration of counterions, post-translational modifications of histones and binding of structural non-histone proteins.
Antonio Rodríguez-Campos +1 more
doaj +1 more source
Jurnal Pendidikan Matematika dan IPA, 2019 Abstrak. Validitas reaksi qPCR untuk aplikasi teknologi diagnostik ataupun deteksi sangat penting. Reaksi qPCR yang valid, akan memberikan hasil dengan tingkat kepercayaan yang tinggi. Untuk mengukur validitas reaksi dapat dilakukan dengan mengukur nilai
Farida Mirnawati +9 more
doaj +1 more source