Results 71 to 80 of about 299,739 (289)

Cooperative Unfolding of Residual Structure in Heat Denatured Proteins by Urea and Guanidinium Chloride. [PDF]

PLoS ONE, 2015
The denatured states of proteins have always attracted our attention due to the fact that the denatured state is the only experimentally achievable state of a protein, which can be taken as initial reference state for considering the in vitro folding and
Ritu Singh, Md Imtaiyaz Hassan, Asimul Islam, Faizan Ahmad   +3 more
doaj   +1 more source

Flow-cytometric cell sorting coupled with UV mutagenesis for improving pectin lyase expression

Frontiers in Bioengineering and Biotechnology, 2023
Introduction: Alkaline pectin lyase is an important enzyme with a wide range of applications in industrial production, It has been widely used in many important fields such as fruit juice processing and extraction, the dyeing and processing of cotton and
Ke Fang, Ke Fang, Jun Ma, Jun Ma, Xinyu Wang, Xinyu Wang, Ziting Xu, Ziting Xu, Ziyang Zhang, Ziyang Zhang, Piwu Li, Piwu Li, Ruiming Wang, Ruiming Wang, Junqing Wang, Junqing Wang, Chuying Sun, Chuying Sun, Ziyang Dong, Ziyang Dong   +19 more
doaj   +1 more source

Multidimensional OMICs reveal ARID1A orchestrated control of DNA damage, splicing, and cell cycle in normal‐like and malignant urothelial cells

Molecular Oncology, EarlyView.
Loss of the frequently mutated chromatin remodeler ARID1A, a subunit of the SWI/SNF cBAF complex, results in less open chromatin, alternative splicing, and the failure to stop cells from progressing through the cell cycle after DNA damage in bladder (cancer) cells. Created in BioRender. Epigenetic regulators, such as the SWI/SNF complex, with important
Rebecca M. Schlösser, Florian Krumbach, Eyleen Corrales, Geoffroy Andrieux, Christian Preisinger, Franziska Liss, Alexandra Golzmann, Melanie Boerries, Kerstin Becker, Ruth Knüchel, Stefan Garczyk, Bernhard Lüscher   +11 more
wiley   +1 more source

Localization of Disulfide Bonds in Ribonuclease Using Low pH Trypsin and LC-ESI-QTOF-MS

CHIMIA
We evaluated a method to localize disulfide bonds in bovine pancreatic ribonuclease (RNase) by applying low pH trypsin protein digestion with a bottom-up LC-ESI-QTOF-MS approach.
Amélie Bornex, Saša M. Miladinović
doaj   +1 more source

Identification and characterization of a novel phosphodiesterase from the metagenome of an Indian coalbed. [PDF]

PLoS ONE, 2015
Phosphoesterases are involved in the degradation of organophosphorus compounds. Although phosphomonoesterases and phosphotriesterases have been studied in detail, studies on phosphodiesterases are rather limited.
Durgesh Narain Singh, Ankush Gupta, Vijay Shankar Singh, Rajeev Mishra, Suneel Kateriya, Anil Kumar Tripathi   +5 more
doaj   +1 more source

RNase H enables efficient repair of R-loop induced DNA damage. [PDF]

, 2016
R-loops, three-stranded structures that form when transcripts hybridize to chromosomal DNA, are potent agents of genome instability. This instability has been explained by the ability of R-loops to induce DNA damage. Here, we show that persistent R-loops
Amon, Jeremy D, Koshland, Douglas
core   +1 more source

Escape from TGF‐β‐induced senescence promotes aggressive hallmarks in epithelial hepatocellular carcinoma cells

Molecular Oncology, EarlyView.
Chronic TGF‐β exposure drives epithelial HCC cells from a senescent state to a TGF‐β resistant mesenchymal phenotype. This transition is characterized by the loss of Smad3‐mediated signaling, escape from senescence, enhanced invasiveness and metastatic potential, and upregulation of key resistance modulators such as MARK1 and GRM8, ultimately promoting
Minenur Kalyoncu, Dilara Demirci, Sude Eris, Bengisu Dayanc, Ece Cakiroglu, Merve Basol, Merve Uysal, Gulcin Cakan‐Akdogan, Fang Liu, Mehmet Ozturk, Gökhan Karakülah, Serif Senturk   +11 more
wiley   +1 more source

1H-NMR investigation of the interaction between RNase T1 and a novel substrate analog, 2′-deoxy-2′-fluoroguanylyl-(3′–5′)uridine [PDF]

, 1988
The interaction between RNase T1 and a non-hydrolysable substrate analog, 2′-deoxy-2′-fluoroguanylyl-(3′–5′)uridine (GfpU), was investigated using 1H-NMR spectroscopy.
Ikehara, Morio, Inagaki, Fuyuhiko, Miyazawa, Tatsuo, Shibata, Yasuyuki, Shimada, Ichio   +4 more
core   +1 more source

Duplex DNA from Sites of Helicase-Polymerase Uncoupling Links Non-B DNA Structure Formation to Replicative Stress [PDF]

, 2020
BACKGROUND: Replication impediments can produce helicase-polymerase uncoupling allowing lagging strand synthesis to continue for as much as 6 kb from the site of the impediment.
Abdelhamid, Mahmoud A S, Amparo, Camille, Bedell, Victoria, Clark, Jarrod, Martella, Marianna, Murata-Collins, Joyce L, Pichiorri, Flavia, Smith, Steven S, Waller, Zoë A E, Warner, Emily F   +9 more
core   +2 more sources

Tumor clusters with divergent inflammation and human retroelement expression determine the clinical outcome of patients with serous ovarian cancer

Molecular Oncology, EarlyView.
Analysis of treatment‐naïve high‐grade serous ovarian carcinoma (HGSOC) and control tissues for ERVs, LINE‐1 (L1), inflammation, and immune checkpoints identified five clusters with diverse patient recurrence‐free survivals. An inflammation score was calculated and correlated with retroelement expression, where one novel cluster (Triple‐I) with high ...
Laura Glossner, Markus Eckstein, Christoph Mark, Matthias W. Beckmann, Arndt Hartmann, Pamela L. Strissel, Reiner Strick   +6 more
wiley   +1 more source