Localization of Disulfide Bonds in Ribonuclease Using Low pH Trypsin and LC-ESI-QTOF-MS
CHIMIAWe evaluated a method to localize disulfide bonds in bovine pancreatic ribonuclease (RNase) by applying low pH trypsin protein digestion with a bottom-up LC-ESI-QTOF-MS approach.
Amélie Bornex, Saša M. Miladinović
doaj +1 more source
Identification and characterization of a novel phosphodiesterase from the metagenome of an Indian coalbed. [PDF]
PLoS ONE, 2015 Phosphoesterases are involved in the degradation of organophosphorus compounds. Although phosphomonoesterases and phosphotriesterases have been studied in detail, studies on phosphodiesterases are rather limited.
Durgesh Narain Singh +5 more
doaj +1 more source
Rocaglates convert DEAD-box protein eIF4A into a sequence-selective translational repressor. [PDF]
, 2016 Rocaglamide A (RocA) typifies a class of protein synthesis inhibitors that selectively kill aneuploid tumour cells and repress translation of specific messenger RNAs.
Floor, Stephen N +2 more
core +1 more source
Molecular Oncology, EarlyView.Human cytomegalovirus infection is common in normal prostate epithelium, prostate tumor tissue, and prostate cancer cell lines. CMV promotes cell survival, proliferation, and androgen receptor signaling. Anti‐CMV pharmaceutical compounds in clinical use inhibited cell expansion in prostate cancer models in vitro and in vivo, motivating investigation ...
Johanna Classon +13 more
wiley +1 more source
Human THO–Sin3A interaction reveals new mechanisms to prevent R-loops that cause genome instability [PDF]
, 2017 R-loops, formed by co-transcriptional DNA–RNA hybrids and a displaced DNA single strand (ssDNA), fulfill certain positive regulatory roles but are also a source of genomic instability.
Aguilera López, Andrés +5 more
core +1 more source
β‐TrCP overexpression enhances cisplatin sensitivity by depleting BRCA1
Molecular Oncology, EarlyView.Low levels of β‐TrCP (Panel A) allow the accumulation of BRCA1 and CtIP, which facilitate the repair of cisplatin‐induced DNA damage via homologous recombination (HR) and promote tumor cell survival. In contrast, high β‐TrCP expression (Panel B) leads to BRCA1 and CtIP degradation, impairing HR repair, resulting in persistent DNA damage and apoptosis ...
Rocío Jiménez‐Guerrero +8 more
wiley +1 more source
Engineering ligand-responsive RNA controllers in yeast through the assembly of RNase III tuning modules [PDF]
, 2011 The programming of cellular networks to achieve new biological functions depends on the development of genetic tools that link the presence of a molecular signal to gene-regulatory activity.
An +37 more
core +3 more sources
FEBS Open Bio, EarlyView.Cleavable N‐terminal Thioredoxin fusion enabled soluble expression and purification of otherwise insoluble SARS‐CoV‐2 Nucleocapsid (N) protein. A four‐step purification strategy yielded highly homogeneous, RNA‐free N protein. Binding assays showed high RNA affinity (Kd ~ 28 nm). The study will facilitate high‐resolution structural studies of N protein,
Shweta Singh, Gagan D. Gupta
wiley +1 more source
RNA-dependent association with myosin IIA promotes F-actin-guided trafficking of the ELAV-like protein HuR to polysomes [PDF]
, 2013 The role of the mRNA-binding protein human antigen R (HuR) in stabilization and translation of AU-rich elements (ARE) containing mRNAs is well established.
Doller, Anke +3 more
core +1 more source
Analysis of the regulation of undecaprenyl diphosphate dephosphorylation in Escherichia coli
FEBS Open Bio, EarlyView.BacA, PgpB, and YbjG phosphatases are involved in undecaprenyl phosphate (C55P) synthesis in Escherichia coli. We analyzed the lipid contents and the gene expression in the gene‐disruption strains. Undecaprenyl diphosphate (C55PP) level increased in the bacA, ybjG double‐disruption strain, but C55P levels were similar in all strains.
Tomotaka Jitsukawa +2 more
wiley +1 more source