Results 151 to 160 of about 852,932 (297)

Engineering Compact Base Editors by AlphaFold‐Guided Mutation Scan and Escherichia coli‐Based Tri‐Selection

Advanced Science, EarlyView.
A miniaturized deaminase SsdAtox was scanned with AlphaFold to identify DNA binding pocket hot spots. Site‐saturation mutagenesis at gatekeeper residue K31 yielded ten‐fold activity enhancement. Trinity Screen, an E. coli‐based three‐in‐one platform selecting for high activity and reduced double‐strand breaks, enabled combinatorial evolution at DNA ...
Ryeo Gang Son, Goeun Kim, Jungjoon K. Lee   +2 more
wiley   +1 more source

Advanced competence in intensive care unit: expectations, role ambiguity between physicians and nurses in intensive care units. Multi - method survey.

Acta Biomed, 2022
Carbone R, Ferrari S, Belperio S, Bravi S, Mancinelli C, Soave E, Di Bartolomeo M, Andreassi L, Perondi L, Tamba M, Astolfi M, Ossola C, Artioli G, Sarli L.   +13 more
europepmc   +1 more source

Alternating High‐Fat and Polysaccharide Diets Modulates Gut Phage‐Bacterial Interplay

Advanced Science, EarlyView.
This study reveals how alternating high‐fat and polysaccharide diets reshape the human gut virome and enhance phage‐bacteria interactions. Using large‐scale metagenomic meta‐analysis and a time‐resolved mouse model, the authors show that diets strongly modulate phage abundance, lifestyle, and gene exchange, offering new insights into nutrition‐guided ...
Fengxiang Zhao, Ruiqi Zhang, Rujun Wei, Huimin Fan, Yongfei Hu, Wenyu Shi, Jinfeng Wang   +6 more
wiley   +1 more source

Effect of Role Ambiguity and Fatigue on Employee Performance in Pelamonia Hospital, Makassar, Indonesia. [PDF]

Iran J Public Health, 2020
Adam A, Alim A, Maulana D.
europepmc   +1 more source

Respiratory‐Limbic Coupling via a Thalamic Circuit Alleviates Anxiety

Advanced Science, EarlyView.
This study delineates a conserved preBötCGlu→PVT→CeA circuit that gates anxiety and respiration. Activation of this circuit is anxiolytic and respiratory‐stabilizing, while its inhibition has the opposite effect. Mechanistically, PVT exerts its anxiolytic action via a disinhibitory microcircuit: its inputs preferentially target CeL GABAergic neurons ...
Shangyu Bi, Xiaoyi Wang, Huichun Luo, Ziteng Yue, Tianjiao Deng, Yuhang Liu, Xinxin Chen, Jianxu Zhao, Luo Shi, Ning Ma, Lingyan Mao, Jing Ding, Jiwen Xu, Ti‐Fei Yuan, Sheng Wang, Fang Yuan   +15 more
wiley   +1 more source

Role ambiguity and nursing interns' achievement of clinical rotation goals: a correlational study. [PDF]

BMC Nurs
Abdelaziz MMA, Saleh MSM, Aysha ZM, Shaheen RAEA.   +3 more
europepmc   +1 more source

The role of intuition and reasoning in driving aversion to risk and ambiguity [PDF]

Using information on a large sample of retail investors and experimental data we find that risk aversion and risk ambiguity are correlated: individuals who dislike risk also dislike ambiguity. We show that what links these traits is the way people handle
Jeffrey V. Butler, Luigi Guiso, Tullio Jappelli   +2 more
core  

SKOOTS: Skeleton‐Oriented Object Segmentation for Mitochondria in High‐Resolution Cochlear EM Datasets

Advanced Science, EarlyView.
Skeleton‐oriented object segmentation (SKOOTS) introduces a new strategy for 3D mitochondrial instance segmentation by predicting explicit skeletons rather than relying on boundary cues. This approach enables robust analysis of densely packed organelles in large FIB‐SEM datasets.
Christopher J. Buswinka, Richard T. Osgood, Hidetomi Nitta, Artur A. Indzhykulian   +3 more
wiley   +1 more source

vEMRec: High‐Resolution Volume Electron Microscopy Reconstruction Based on Structure‐Preserving and High‐Fidelity 3D Alignment

Advanced Science, EarlyView.
vEMRec is a frequency‐adaptive computational framework for three‐dimensional alignment in volume electron microscopy. It integrates feature‐based rigid alignment with Gaussian filter‐guided elastic registration to correct rigid misalignments and nonlinear distortions while preserving structural fidelity.
Zhenbang Zhang, Hongjia Li, Zhongjun Yang, Zhiqiang Xu, Duanchen Sun, Xin Gao, Fa Zhang, Renmin Han   +7 more
wiley   +1 more source

Bridging the gap: a qualitative study of role ambiguity, educational misalignment, and systemic barriers in China's advanced practice nurse training. [PDF]

BMC Nurs
Yang JY, Wan XL, Hu JP, Yin XS, Li XB.
europepmc   +1 more source