Results 211 to 220 of about 529,159 (305)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

First Report and Biological Characterization of <i>Penicillium crustosum</i> Causing Root Rot in <i>Polygonatum kingianum</i> (Yunnan, China). [PDF]

Plants (Basel)
Zhang MX, Chen ZH, Wang LH, Yang XY, Zhu YY, Zhao Y.   +5 more
europepmc   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Drought adaptation in spring wheat seedlings relies on coordinated deep root architecture and cortical tissue allocation. [PDF]

Front Plant Sci
Li X, Guo C, Bai C, Wang S, Gao X, Huo J, Cui F, Fan H, Bao X, Zhao C.   +9 more
europepmc   +1 more source

C2α‐carbanion‐protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2‐hydroxyacyl‐CoA lyase

FEBS Open Bio, EarlyView.
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn, Barbara Seroka, Ryszard Lazny, Zenon Lotowski, Thore Rohwerder   +4 more
wiley   +1 more source

Comparative evaluation of apical root resorption in adult patients treated with clear aligners and fixed orthodontic appliances using CBCT. [PDF]

Acta Odontol Scand
Issrani R, Alshammari AH, Alrwuili MR, Aljabab MA, Alenzi NA, Joshi P, Aldoseri YT.   +6 more
europepmc   +1 more source

On the characteristic roots of matric polynomials [PDF]

Bulletin of the American Mathematical Society, 1936
openaire   +3 more sources

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Enhancing graft stability in coronally advanced tunnel with connective tissue graft for Cairo RT1 and RT2 gingival recession: a case series. [PDF]

J Surg Case Rep
Khursheed DA.
europepmc   +1 more source

Moments and characteristic roots. II

Numerische Mathematik, 1968
openaire   +1 more source