Results 231 to 240 of about 16,889,900 (317)

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Nonsense-Mediated Decay mRNA Quality Control System Is Essential for Root Development and Efficient Root Nodule Symbiosis in Medicago truncatula. [PDF]

Plant Cell Environ
Biró JB, Péter C, Domonkos Á, Hegedűs Z, Lakatos L, Rana D, Gyula P, Silhavy D, Kaló P.   +8 more
europepmc   +1 more source

UiO‐66 metal–organic frameworks in biomedicine: From structural tunability to bioimaging, photodiagnostics, and photodynamic cancer therapy

FEBS Open Bio, EarlyView.
UiO‐66(Zr) metal–organic frameworks are chemically stable, biocompatible, and highly tunable nanomaterials. Their modular structure enables controlled drug delivery, multimodal bioimaging, and light‐activated photodynamic therapy, supporting integrated diagnostic and therapeutic (theranostic) applications in cancer and biomedical research.
Veronika Huntošová, Grigorii Rakhalskii, Miroslav Almáši   +2 more
wiley   +1 more source

Cellular and Systemic Toxicity Induced by Sunscreen and Paraben Mixtures and the Implications for Root Development in Cultivated Plants. [PDF]

J Toxicol
Maehashi AE, Galdino EM, de Almeida EA, Santo DE, Henrich CL, da Cruz B, Dusman E, de Oliveira DCDS, Tractz GT, Gonzalez RDS, Junior OV, Downs CA, Peron AP.   +12 more
europepmc   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Selective autophagy receptor VISP1 enhances cytokinin signaling by modulating the Type-A/B ARR feedback loop during root development. [PDF]

Sci Adv
Tong X, Feng YL, Guo ZH, Wang XB, Wang Y.   +4 more
europepmc   +1 more source

Small RNA pathways in mammalian oocytes

FEBS Open Bio, EarlyView.
Three distinct small RNA pathways operate in mammalian oocytes: RNAi interference (RNAi), the microRNA (miRNA) pathway, and the PIWI‐associated RNA (piRNA) pathway. These pathways use small RNAs to guide sequence‐specific repression and contribute to oocyte biology by targeting genes and mobile elements or appear insignificant since different ...
Petr Svoboda, Josef Pasulka
wiley   +1 more source

Polar-localized EXO70G1 regulates root development in Arabidopsis thaliana. [PDF]

J Integr Plant Biol
Li J, Liu Z, Gong L, Zhao S, Lu Q, Gao S, Jiang S, Liu X, Ma L, Duan G, Cui D, Li S.   +11 more
europepmc   +1 more source

Hyperactive ice‐binding proteins stabilize cell membranes and improve resistance to dehydration stress in Caenorhabditis elegans

FEBS Open Bio, EarlyView.
TisIBP8, a fungal‐derived hyperactive ice‐binding protein, helps Caenorhabditis elegans survive dehydration. It localizes near cell membranes, reduces cell damage, and helps maintain membrane structure during drying. These results suggest that ice‐binding proteins can protect cells from dehydration stress as well as freezing stress.
Daiki Shimose, Kotaro Ozaki, Ryohei Kuriyama, Yuka Ikemoto, Kazuhiro Mio, Yuji C. Sasaki, Tatsuya Arai, Sakae Tsuda, Yoichi Shinkai, Masahiro Kuramochi   +9 more
wiley   +1 more source