Results 121 to 130 of about 324,440 (307)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

open access: yesFEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point   +7 more
wiley   +1 more source

Rookery Building

open access: yes, 1885
Light court and stair by Frank Lloyd Wright: vertical view of the space, looking towards the iron stair; By mixing modern building techniques such as metal framing, fireproofing, elevators and plate glass, together with traditional ones such as brick ...
Burnham and Root, Wright, Frank Lloyd
core  

The Accuracy of Custom-Made Milled Metal Posts as Compared to Conventional Cast Metal Posts

open access: yesDentistry Journal
Aim: The aim of this study was to compare the fitting accuracy of custom-made metal posts and cores fabricated by half-digital and milling technique to that of conventional cast posts fabricated by direct technique.
Tabarak M. AL-Rubaye, Emad S. Elsubeihi
doaj   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

open access: yesFEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Composition Operators on the Little Lipschitz Space of a Rooted Tree

open access: yesMediterranean Journal of Mathematics
In this work, we study the composition operators on the little Lipschitz space ${\mathcal L}_0$ of a rooted tree $T$, defined as the subspace of the Lipschitz space ${\mathcal L}$ consisting of the complex-valued functions $f$ on $T$ such that $$\lim_{|v|\to\infty}|f(v)-f(v^-)|=0,$$ where $v^-$ is the vertex adjacent to the vertex $v$ in the path from ...
Colonna, Flavia   +1 more
openaire   +2 more sources

Nuclear pore links Fob1‐dependent rDNA damage relocation to lifespan control

open access: yesFEBS Open Bio, EarlyView.
Damaged rDNA accumulates at a specific perinuclear interface that couples nucleolar escape with nuclear envelope association. Nuclear pores at this site help inhibit Fob1‐induced rDNA instability. This spatial organization of damage handling supports a functional link between nuclear architecture, rDNA stability, and replicative lifespan in yeast.
Yamato Okada   +5 more
wiley   +1 more source

Armstrong Air and Space Museum aerial photograph

open access: yes, 1976
Aerial photograph showing the Armstrong Air and Space Museum in Wapakoneta, Ohio. The museum opened to the public July 20, 1972, honoring Neil Armstrong, a Wapakoneta native and the first man to set foot on the moon.
Root, Thomas (Photographer)
core  

C2α‐carbanion‐protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2‐hydroxyacyl‐CoA lyase

open access: yesFEBS Open Bio, EarlyView.
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn   +4 more
wiley   +1 more source

Efficient TV white space filter bank transceiver

open access: yes, 2012
Future devices operating in the TV white space (TVWS) spectrum will require to access different bands at different locations and times in order to avoid interference to incumbent users, requiring agility and sufficient spectral masks to satisfy ...
Crockett, Louise   +9 more
core   +1 more source

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