Results 51 to 60 of about 175,165 (277)

A COMPARISON OF PRE-ROUNDING AND POST-ROUNDING FLOATING POINT DIVIDE ORDERS [PDF]

open access: yes, 2008
This paper addresses the advantages and disadvantages of pre-rounding vs. post-rounding in a floating-point register. In this day when high speed computers with long registers are the norm, one may think that a rounding scheme is nearly irrelevant ...
Kettler, Paul C.
core  

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

open access: yesFEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point   +7 more
wiley   +1 more source

Differential regulation of ZFAS1 splice variants by endoplasmic reticulum stress in hepatocyte cell lines

open access: yesFEBS Open Bio, EarlyView.
ZFAS1 is a lncRNA promoting cell proliferation and migration, exhibiting high expression in various cancers. It is conserved, widely expressed, and produces multiple splice variants with unclear roles. We identified several splice variants in hepatocyte models, and found that inhibiting or suppressing regulators of the unfolded protein response (PERK ...
Sébastien Soubeyrand   +2 more
wiley   +1 more source

Error Analysis and Model Adaptivity for Flows in Gas Networks

open access: yesAnalele Stiintifice ale Universitatii Ovidius Constanta: Seria Matematica, 2018
In the simulation and optimization of natural gas flow in a pipeline network, a hierarchy of models is used that employs different formulations of the Euler equations. While the optimization is performed on piecewise linear models, the flow simulation is
Stolwijk Jeroen J., Mehrmann Volker
doaj   +1 more source

Rounding error analysis of the classical Gram-Schmidt orthogonalization process

open access: yesNumerische Mathematik, 2005
Given an \(m\times n\) matrix \(A\) of full column rank, this paper is concerned with the numerical properties of the classical Gram-Schmidt process (CGS) for computing an orthonormal basis of the space spanned by the columns of \(A\). Based on a backward error bound for the upper triangular factor produced by CGS, it is proven that the loss of ...
Giraud, L.   +3 more
openaire   +2 more sources

HIV‐1 establishes immediate latency in T cells expressing the viral Nef protein

open access: yesFEBS Open Bio, EarlyView.
Nef is a viral protein often omitted from HIV‐1 reporter viruses. Consequently, its role in viral latency is unclear. We developed three novel dual reporter HIV‐1 derivatives that express Nef and allow for detection of latent and productive infection. Using these reporters, we show that Nef does not affect the establishment of immediate viral latency ...
Cindy Lam, Ivan Sadowski
wiley   +1 more source

Optimal Number of Choices in Rating Contexts

open access: yes, 2018
In many settings people must give numerical scores to entities from a small discrete set. For instance, rating physical attractiveness from 1--5 on dating sites, or papers from 1--10 for conference reviewing.
Ganzfried, Sam, Yusuf, Farzana
core   +1 more source

Development of human monoclonal antibodies against TARM1 by yeast display

open access: yesFEBS Open Bio, EarlyView.
Human monoclonal antibodies against TARM1 are generated by yeast display‐guided selection. These antibodies bind to soluble and cell‐surface forms of TARM1. Also, these antibodies exhibit agonistic activity in the NFAT‐GFP reporter assay, indicating that TARM1 signaling can be functionally modulated by antibodies and suggesting TARM1 as a potential ...
Rikio Yabe   +5 more
wiley   +1 more source

FPU-Supported Running Error Analysis

open access: yesActa Polytechnica, 2010
A-posteriori forward rounding error analyses tend to give sharper error estimates than a-priori ones, as they use actual data quantities. One of such a-posteriori analysis – running error analysis – uses expressions consisting of two parts; one generates
T. Zahradnický, R. Lórencz
doaj  

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

open access: yesFEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen   +9 more
wiley   +1 more source

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