Results 81 to 90 of about 608 (160)

Introduction to special issue: Advancing disease ecology through eDNA monitoring of infectious agents

open access: yesEnvironmental DNA, Volume 6, Issue 1, January–February 2024.
AbstractThis special issue focuses on the applications of environmental DNA (eDNA) sequencing for the detection and monitoring of disease‐causing agents, including viruses, bacteria, protozoans, myxozoans, fungi, trematodes, and arthropods. We explore the impact of eDNA technologies, such as metabarcoding and qPCR, in understanding the dynamics of ...
Jasmine Childress   +2 more
wiley   +1 more source

Expression Analysis of Interferon-Stimulated Gene 15 in the Rock Bream Oplegnathus fasciatus against Rock Bream Iridovirus (RSIV) Challenge

open access: yesDevelopment & Reproduction, 2017
Interferon-stimulated gene 15 (ISG15) is known to interfere with viral replication and infection by limiting the viral infection of cells. Interferon-stimulated gene 15 (ISG15) interferes with viral replication and infectivity by limiting viral infection in cells. It also plays an important role in the immune response.
Kim, Kyung-Hee   +9 more
openaire   +3 more sources

RsiV mutants that cannot bind lysozyme are less efficient at inhibiting lysozyme muramidase activity.

open access: yes, 2016
Peptidoglycan from M. lysodekticus was combined with lysozyme (20 μg/ ml) and purified RsiV or RsiV mutants at a molar ratio of 1:1 with lysozyme. The OD450 was monitored every minute for 30 minutes to determine lysozyme specific activity as previously ...
Jon C. Houtman (638203)   +5 more
core   +1 more source

Content Validity of the ADHD Rating Scale (ADHD RS-IV) and Adult ADHD Self-Report Scale (ASRS) in Phenylketonuria

open access: yesJournal of Inborn Errors of Metabolism and Screening, 2016
The ADHD Rating Scale (ADHD RS-IV; parent report) and Adult ADHD Self-Rating Scale (ASRS; self-report) are validated instruments for measuring symptoms of attention-deficit/hyperactivity disorder (ADHD).
Kathleen W. Wyrwich PhD   +6 more
doaj   +1 more source

In vitro reconstitution of site-1 cleavage requires signal peptidase and lysozyme.

open access: yes, 2014
Lane 1 contains 0.3 mg/mL purified HEW lysozyme; Lanes 2–7 contain cell free transcription/translation products derived using plasmids containing the gene(s) noted above the gel.
Jon C. Houtman (638203)   +5 more
core   +1 more source

Aligned partial (68 bp) hypothetical protein ISKNV 104R (A) for 23 megalocytivirus strains illustrating the in silico specificity of the qPCR primers and probe of ISKNV TaqMan qPCR assay (this study).

open access: yes, 2023
Aligned partial major capsid protein (MCP) for the same 23 megalocytivirus strains illustrating the in silico specificity of the qPCR primers RSIV RT F/R and RSIV probe (B; [69]) and C1073/C1074 (C; [48]).
Kuttichantran Subramaniam (9981904)   +5 more
core   +1 more source

ELECTRON MICROSCOPIC ANALYSIS OF ENLARGED CELLS DERIVED FROM RED SEA BREAM IRIDOVIRUS (RSIV)-INFECTED CULTURED GRUNT FIN (GF) CELLS

open access: yes, 2013
Red sea bream iridovirus (RSIV) has been known to induce cellular enlargement as cytophatic effect (CPE) in cultured cell line. In the present study, grunt fin (GF) cells were treated with RSIV.
Mahardika, Ketut, Ketut Mahardika
core   +1 more source

Establishment of a cell line from kidney of seabass, Lates calcarifer (Bloch) [PDF]

open access: yesSongklanakarin Journal of Science and Technology (SJST), 2003
Primary cell culture from caudal fin and kidney of seabass (Lates calcarifer Bloch) using tissue explant method were cultured in three different medias with various salt concentrations.
Phromkunthong, W.   +3 more
doaj  

Sensing Extracellular Stress: Key Features of the Anti-Sigma Factor RsiV in Sensing Lysozyme [PDF]

open access: yes, 2017
Bacillus subtilis is a soil dwelling organism which uses alternative extracytoplasmic function (ECF) sigma factors that respond to environmental stressors encountered by a cell. Upon encountering a signal, ECF sigma factors are activated and bind RNAP to
Kies, Paige
core  

RsiVS169W, P259A, Y261A is unable to bind lysozyme.

open access: yes, 2016
Representative run of isothermal titration calorimetry (ITC) experiments conducted with A. 6xHis-2xFlag-RsiV59-285 and HEW lysozyme or B. 6xHis-2xFlag-RsiV59-285, S169W, P259A, Y261A and HEW lysozyme.
Jon C. Houtman (638203)   +5 more
core   +1 more source

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