Results 171 to 180 of about 491,020 (188)

Qualitätsmanagement in der RT-qPCR

BIOspektrum, 2012
Several aspects in the numerous steps of a reverse transcription (RT) quantitative PCR may interfere with the result’s validity. Therefore, before starting the proper investigation, a particular assay establishment is required.
Catrin Wernicke, Philipp Franke, Lars Radke, Stephan Berge, Marcus Frohme   +4 more
openaire   +1 more source

Whole-Genome RT-qPCR MicroRNA Expression Profiling

2011
MicroRNAs (miRNAs) are small noncoding RNA molecules that function as negative regulators of gene expression. They are essential components of virtually every biological process and deregulated miRNA expression has been reported in a multitude of human diseases including cancer.
Pieter, Mestdagh, Stefaan, Derveaux, Jo, Vandesompele   +2 more
openaire   +2 more sources

Multigene Combined Detection by RT-qPCR Using Cytological Specimens

Acta Cytologica, 2021
<b><i>Objective:</i></b> The aim of the study was to investigate the mutation status of multiple driver genes by RT-qPCR and their significance in advanced lung adenocarcinoma using cytological specimens. <b><i>Materials and Methods:</i></b> 155 cytological specimens that had been diagnosed with lung ...
Yang, Ma, Jingxia, Zhao, Yun, Du, Rui, Wang, Xiaokun, Ji, Juan, Wu, Xiao, Guo, Ying, Liu, Yan, Zhang   +8 more
openaire   +2 more sources

Quantitation of MicroRNAs by Real-Time RT-qPCR

2010
MicroRNAs (miRNAs) are ∼22 nucleotide regulatory RNA molecules that play important roles in controlling developmental and physiological processes in animals and plants. Measuring the level of miRNA expression is a critical step in methods that study the regulation of biological functions and that use miRNA profiles as diagnostic markers for cancer and ...
Caifu, Chen, Ruoying, Tan, Linda, Wong, Richard, Fekete, Jason, Halsey   +4 more
openaire   +2 more sources

Stem-Loop RT-qPCR for MicroRNA Expression Profiling

2011
Quantification of the microRNAs (miRNAs) in cells or tissues is a crucial step in understanding their biological functions. Development of the stem-loop reverse transcription procedure and TaqMan(®) miRNA assays enables accurate detection of miRNA expression levels by quantitative PCR.
James, Hurley, Doug, Roberts, Andrew, Bond, David, Keys, Caifu, Chen   +4 more
openaire   +2 more sources

Gene Expression Analysis in Bacteria by RT-qPCR

2019
Reverse-transcription quantitative real-time polymerase chain reaction (RT-qPCR) using fluorescent DNA-binding dyes is now a gold-standard methodology to study bacterial gene expression through relative quantitation of target mRNAs under specific experimental conditions, and recent developments in the technology allow for gene expression analysis in ...
Danilo J P G, Rocha, Thiago L P, Castro, Eric R G R, Aguiar, Luis G C, Pacheco   +3 more
openaire   +2 more sources

RT-qPCR work-flow for single-cell data analysis

Methods, 2013
Individual cells represent the basic unit in tissues and organisms and are in many aspects unique in their properties. The introduction of new and sensitive techniques to study single-cells opens up new avenues to understand fundamental biological processes.
Anders, Ståhlberg, Vendula, Rusnakova, Amin, Forootan, Miroslava, Anderova, Mikael, Kubista   +4 more
openaire   +2 more sources

Analisi dei miRNA mediante RT-qPCR

2018
Protocollo di analisi dei miRNA mediante RT-qPCR adottato da CNR-IFC (in collaborazione con CNR-IRPPS e Università del Salento) nell'ambito del progetto GILEAD (Fellowship 2015) "Correlazione dell'andamento dei livelli dei linfociti T CD4+ e dell'HIV-RNA in pazienti naïve e relazione con una differente espressione di miRNA, nell'individuazione ...
openaire   +1 more source

Concise RT-qPCR protocol

2023
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RT-qPCR: ESTUDOS DE EXPRESSÃO GÊNICA

2021
Mayara Mota de Oliveira, Anderson Barros Archanjo   +1 more
openaire   +1 more source