Results 171 to 180 of about 1,081,218 (337)

Analysis of Treacher Collins syndrome 4‐associated mutations in Schizosaccharomyces pombe

FEBS Open Bio, EarlyView.
Fission yeast models carrying Treacher Collins syndrome type 4‐associated mutations reveal that impaired processivity of RNA polymerase I leads to defective rRNA transcription. This study highlights the essential role of a conserved arginine residue in Pol I elongation and provides mechanistic insight into the pathogenesis of ribosomopathies.
Kei Kawakami, Hiroaki Kato
wiley   +1 more source

Microrna response of primary human macrophages to Arcobacter Butzleri infection [PDF]

, 2016
The role of microRNAs (miRNAs) in infectious diseases is becoming more and more apparent, and the use of miRNAs as a diagnostic tool and their therapeutic application has become the major focus of investigation.
Alter, Thomas, Backes, Christina, Bruegge, Jennifer zur, Einspanier, Ralf, Franke, Andre, Gölz, Greta, Hemmrich-Stanisak, Georg, Keller, Andreas, Scharek-Tedin, Lydia, Sharbati, Soroush   +9 more
core   +3 more sources

WetLab-2 RT-qPCR procedure v1

, 2017
Procedure used for RT-qPCR in the WetLab-2 study as well as procedure for preparation of lyophilized ...
openaire   +1 more source

Establishment of a coculture system for Porphyromonas gingivalis and head and neck squamous cell carcinoma using spheroid culture and LATS inhibition

FEBS Open Bio, EarlyView.
We established a spheroid coculture system enabling viable Porphyromonas gingivalis–HNSCC interactions under normoxic conditions. Inhibition of LATS1/2 maintains tumor cells in an undifferentiated state, which may promote spheroid growth and create a more permissive environment for bacterial persistence.
Yurika Nakajima, Shogo Okazaki, Muneaki Tamura, Shuichi Sato, Kenichi Imai   +4 more
wiley   +1 more source

A real-time, quantitative PCR method using hydrolysis probes for the monitoring of Plasmodium falciparum load in experimentally infected human volunteers

Malaria Journal, 2011
Background The accurate quantification of Plasmodium falciparum parasite numbers by PCR is an important tool for monitoring growth kinetics in subjects infected and subsequently treated with anti-malarial agents.
Trenholme Katharine, Nissen Michael D, Whiley David M, Bialasiewicz Seweryn, Peatey Chris, Tozer Sarah J, Rockett Rebecca J, Mc Carthy James S, Sloots Theo P   +8 more
doaj   +1 more source

ATP13A2 is involved in intracellular polyamine transport in lung epithelial cells

FEBS Open Bio, EarlyView.
Spermidine transport in lung epithelial cells involves the polyamine transporter ATP13A2. Cell proliferation is associated with the upregulation of ATP13A2. Polyamines are present in all living cells and are implicated in various crucial cellular processes such as proliferation, apoptosis and autophagy.
Yuta Hatori, Kohei Kawabata, Takanori Kubo, Takeo Kitazawa, Sae Kanai, Madoka Iwashita, Ami Hayashi, Hiroyuki Nishi, Mikihisa Takano   +8 more
wiley   +1 more source

Chronic Myeloid Leukaemia with isolated massive thrombocytosis and BCR‐ABL1 detection failure using RT‐MLPA (positive RT‐qPCR)

eJHaem, 2021
Lucie Coster, Véronique Mansat‐De MAS, Laetitia Largeaud, Sophie Voisin, Jill Corre, Martin Gauthier   +5 more
doaj   +1 more source

Comparison of virus concentration methods for the RT-qPCR-based recovery of murine hepatitis virus, a surrogate for SARS-CoV-2 from untreated wastewater

Science of the Total Environment, 2020
W. Ahmed, P. Bertsch, A. Bivins, K. Bibby, K. Farkas, Amy Gathercole, E. Haramoto, P. Gyawali, A. Korajkic, B. McMinn, Jochen F Mueller, S. Simpson, W. Smith, E. Symonds, K. Thomas, Rory Verhagen, M. Kitajima   +16 more
semanticscholar   +1 more source

KLK7 overexpression promotes an aggressive phenotype and facilitates peritoneal dissemination in colorectal cancer cells

FEBS Open Bio, EarlyView.
KLK7, a tissue kallikrein‐related peptidase, is elevated in advanced colorectal cancer and associated with shorter survival. High KLK7 levels in ascites correlate with peritoneal metastasis. In mice, KLK7 overexpression increases metastasis. In vitro, KLK7 enhances cancer cell proliferation, migration, adhesion, and spheroid formation, driving ...
Yosr Z. Haffani, Tobias Dreyer, Meriem Naim, Rea Lo Dico, Natalia A. Ignatenko, Viktor Magdolen, Dalila Darmoul   +6 more
wiley   +1 more source

Pioglitazone plus (−)‐epigallocatechin gallate: a novel approach to enhance osteogenic performance in aged bone marrow mesenchymal stem cells

FEBS Open Bio, EarlyView.
Aged human bmMSCs are seeded in the scaffold. Osteoblastic induction can slightly increase cell's bone‐forming activity to produce bone‐like tissues, shown as the sporadic xylenol orange‐stained spots (the lower left image). Notably, pioglitazone plus EGCG co‐treatment dramatically increases cell's bone‐forming activity and bone‐like tissue production (
Ching‐Yun Chen, Jia‐Ci Jhang, Chun‐Yi Peng, Quốc Cường Nguyễn, Feng‐Huei Lin, Hung‐Ming Wu, Shankung Lin   +6 more
wiley   +1 more source