Results 301 to 310 of about 1,081,218 (337)

miRNA Stem-loop RT-qPCR Method

BIO-PROTOCOL
openaire   +1 more source

Cancer Cell‐Intrinsic Cholesterol Induces Lipid‐Associated Macrophage Differentiation via SP1 Palmitoylation to Promote Prostate Cancer Progression

Advanced Science, EarlyView.
Cancer cell‐intrinsic cholesterol promotes the S‐palmitoylation of SP1, increasing its nuclear translocation and driving the transcription and secretion of MDK, which in turn facilitates the differentiation of macrophages into a lipid‐associated phenotype.
Shirong Peng, Weilong Lin, Zean Li, Ruilin Zhuang, Shengmeng Peng, Bingheng Li, Bingliang Chen, Yong Luo, Yuan Ou, Wei Zhuang, Tao Du, Kaiwen Li, Hai Huang   +12 more
wiley   +1 more source

Detection of Lineage IV Peste Des Petits Ruminants Virus by RT-qPCR Assay via Targeting the Hemagglutinin Gene. [PDF]

Viruses
Xu J, Wang Q, Yu J, Wang Y, Li H, Li L, Bao J, Wang Z.   +7 more
europepmc   +1 more source

Extracellular Vesicle‐Delivered tRF‐His‐GTG‐1 Reprograms Neutrophil Lipophagy and Triggers Inflammation in COVID‐19

Advanced Science, EarlyView.
This study identifies platelet‐derived extracellular vesicles as key immunometabolic regulators in COVID‐19. The delivery of tRF‐His‐GTG‐1 to neutrophils activates TLR8mTOR signaling, disrupts lipophagy, and amplifies NET‐mediated inflammation. Importantly, targeting this axis restores neutrophil homeostasis, offering a potential therapeutic strategy ...
Tsai‐Ling Liao, Po‐Yu Liu, Yi‐Ming Chen, Kuo‐Tung Tang, Hung‐Jen Liu, Der‐Yuan Chen   +5 more
wiley   +1 more source

Development of an in-house dual RT-qPCR assay for detecting SFTSV and Hantaan virus simultaneously. [PDF]

Biosaf Health
Xue X, Liu Y, Song C, Liu T, Liu Z, Niu W, Jiang Z, Xu Y, Zhang Y, Lin L, Chen Z.   +10 more
europepmc   +1 more source

Bioprinting of Microtissues Within Mechanically Tunable Support Baths to Engineer Anisotropic Musculoskeletal Tissues

Advanced Science, EarlyView.
This study presents a novel 4D bioprinting platform for engineering biomimetic musculoskeletal grafts. By tuning the mechanical properties of support baths, we enhance tissue fusion, collagen alignment, and cell differentiation. Using this strategy, we successfully fabricate scaled‐up, anisotropic tissues such as meniscus, articular cartilage, and ...
Francesca D. Spagnuolo, Gabriela S. Kronemberger, Daniel J. Kelly   +2 more
wiley   +1 more source

The Impact of the Variability of RT-qPCR Standard Curves on Reliable Viral Detection in Wastewater Surveillance. [PDF]

Microorganisms
Casado-Martín L, Hernández M, Yeramian N, Pérez D, Eiros JM, Valero A, Rodríguez-Lázaro D.   +6 more
europepmc   +1 more source

COVID-19 RT-qPCR-based screening in Austrian schools and incidences in the general population: a Bayesian spatiotemporal analysis. [PDF]

Arch Public Health
Natalia YA, Herzog SA, Neyens T, Zurl CJ, Strenger V, Molenberghs G, Faes C.   +6 more
europepmc   +1 more source

Field-validated multiplex RT-qPCR for simultaneous detection of bovine respiratory syncytial virus and bovine parainfluenza virus-3 in bovine respiratory samples. [PDF]

Front Vet Sci
Zulauf B, Pastey MK.
europepmc   +1 more source

Transcriptomics and network pharmacology reveal the potential mechanism related to integrated stress response in the treatment of osteoporosis by Jiawei Shentong Zhuyu Decoction and verified by RT-qPCR. [PDF]

Front Endocrinol (Lausanne)
Zhao J, Zhang T, Han P, Liu Z, Zhang H, Li F, Han G.   +6 more
europepmc   +1 more source