Results 281 to 290 of about 917,459 (311)

mRNA and microRNA quality control for RT-qPCR analysis

Methods, 2010
The importance of high quality sample material, i.e. non-degraded or fragmented RNA, for classical gene expression profiling is well documented. Hence, the analysis of RNA quality is a valuable tool in the preparation of methods like RT-qPCR and microarray analysis. For verification of RNA integrity, today the use of automated capillary electrophoresis
C, Becker, A, Hammerle-Fickinger, I, Riedmaier, M W, Pfaffl   +3 more
openaire   +2 more sources

TaqMan RT-qPCR for tRNA half quantification

When quantifying tRNA-derived short non-coding RNAs (sncRNAs), two key considerations must be addressed. First, sequencing analyses have revealed significant heterogeneity in the lengths and terminal sequences of tRNA-derived sncRNAs. Second, within the total RNA fraction, these sncRNAs coexist with more abundant mature tRNAs and their precursors (pre ...
Megumi, Shigematsu, Takuya, Kawamura, Yohei, Kirino   +2 more
openaire   +2 more sources

Selection of Reliable Reference Genes for RT-qPCR Analysis

2014
Reference genes have become the method of choice for normalization of qPCR data. It has been demonstrated in many studies that reference gene validation is essential to ensure accurate and reliable results. This chapter describes how a pilot study can be set up to identify the best set of reference genes to be used for normalization of qPCR data.
Jan, Hellemans, Jo, Vandesompele
openaire   +2 more sources

Stem-Loop RT-qPCR for MicroRNA Expression Profiling

2011
Quantification of the microRNAs (miRNAs) in cells or tissues is a crucial step in understanding their biological functions. Development of the stem-loop reverse transcription procedure and TaqMan(®) miRNA assays enables accurate detection of miRNA expression levels by quantitative PCR.
James, Hurley, Doug, Roberts, Andrew, Bond, David, Keys, Caifu, Chen   +4 more
openaire   +2 more sources

Rare Transcripts Quantification by Strand-Specific RT-qPCR

B lymphocytes development is associated with significant immunoglobulin (Ig) gene recombination leading to the establishment of a highly diverse and specific Ig repertoire. Accessibility of Ig genes is tightly regulated and associated with the production of sterile transcripts (noncoding), including low abundant anti-sense (AS) transcripts.
Ophélie Alyssa Martin, Sandrine Le Noir, Eric Pinaud   +2 more
openaire   +2 more sources

Comparison of Electronegative Filtration to Magnetic Bead-Based Concentration and V2G-qPCR to RT-qPCR for Quantifying Viral SARS-CoV-2 RNA from Wastewater

ACS ES&T Water, 2022
Stephan Schürer, Mario Stevenson, Dusica Vidovic   +2 more
exaly  

Concise RT-qPCR protocol

2023
openaire   +1 more source

Quantification of mRNA Expression by RT-qPCR

Transplantation, 2015
openaire   +2 more sources

Variability in RT-qPCR assay parameters indicates unreliable SARS-CoV-2 RNA quantification for wastewater surveillance

Water Research, 2021
Aaron Bivins, W Ahmed, Devrim Kaya
exaly  

COVID-19 Diagnosis: A Comprehensive Review of the RT-qPCR Method for Detection of SARS-CoV-2

Diagnostics, 2022
Debashis Dutta, Sarah Naiyer, Neeraj Soni   +2 more
exaly