Results 271 to 280 of about 795,270 (311)

RT-qPCR for the diagnosis of the vesiculovirus Cocal virus

Archives of Virology, 2020
Cocal virus (COCV) is one of the causative agents of vesicular stomatitis, presenting clinical signs indistinguishable from those caused by foot-and-mouth disease virus (FMDV). Therefore, the differentiation of these two viruses via laboratory diagnosis is essential.
Mariana Lázaro Sales, Mariana Dall’Agnol, Anapolino Macedo de Oliveira, Marcelo Fernandes Camargos, Antônio Augusto Fonseca, Jenner Karlisson Pimenta dos Reis   +5 more
openaire   +2 more sources

Qualitätsmanagement in der RT-qPCR

BIOspektrum, 2012
Several aspects in the numerous steps of a reverse transcription (RT) quantitative PCR may interfere with the result’s validity. Therefore, before starting the proper investigation, a particular assay establishment is required.
Catrin Wernicke, Philipp Franke, Lars Radke, Stephan Berge, Marcus Frohme   +4 more
openaire   +1 more source

Novel duplex RT‐qPCR for animal rabies surveillance

Transboundary and Emerging Diseases, 2022
Rabies is a lethal zoonosis affecting mammals worldwide. Diagnosis of rabies follows international standard protocols, primarily relying on direct immunofluorescence (DI) followed by mouse inoculation test (MIT). WHO recommends molecular biology techniques such as RT-qPCR for replacing MIT to diagnose rabies in animal samples. Recently, a real-time PCR
Guilherme Augusto Minozzo, Thaila Francini Corona, Elaine Cristina Rodrigues da Cruz, Wagner Antonio Chiba de Castro, Louise Bach Kmetiuk, Andrea Pires dos Santos, Alexander Welker Biondo, Irina Nastassja Riediger   +7 more
openaire   +2 more sources

Quantitation of MicroRNAs by Real-Time RT-qPCR

2010
MicroRNAs (miRNAs) are ∼22 nucleotide regulatory RNA molecules that play important roles in controlling developmental and physiological processes in animals and plants. Measuring the level of miRNA expression is a critical step in methods that study the regulation of biological functions and that use miRNA profiles as diagnostic markers for cancer and ...
Caifu, Chen, Ruoying, Tan, Linda, Wong, Richard, Fekete, Jason, Halsey   +4 more
openaire   +2 more sources

Housekeeping genes for RT-qPCR in ovine preimplantation embryos

Zygote, 2020
SummaryHousekeeping genes (HKG) are paramount for accurate gene expression analysis during preimplantation development. Markedly, quantitative reverse transcription polymerase chain reaction (RT-qPCR) in ovine embryos currently lacks HKGs. Therefore, we tested 11 HKGs for RT-qPCR normalization during ovine parthenogenetic preimplantation development ...
Pábola Santos Nascimento, Marcelo Tigre Moura, Roberta Lane Oliveira Silva, Pamela Ramos-Deus, José Carlos Ferreira-Silva, Joane Isis Travassos Veira, Antônio Santana Santos Filho, Sebastião Inocêncio Guido, Cláudio Coutinho Bartolomeu, Ana Maria Benko-Iseppon, Marcos Antonio Lemos Oliveira   +10 more
openaire   +2 more sources

Gene Expression Analysis in Bacteria by RT-qPCR

2019
Reverse-transcription quantitative real-time polymerase chain reaction (RT-qPCR) using fluorescent DNA-binding dyes is now a gold-standard methodology to study bacterial gene expression through relative quantitation of target mRNAs under specific experimental conditions, and recent developments in the technology allow for gene expression analysis in ...
Danilo J P G, Rocha, Thiago L P, Castro, Eric R G R, Aguiar, Luis G C, Pacheco   +3 more
openaire   +2 more sources

Optimal reference genes for RT-qPCR normalization in the newborn

Biotechnic & Histochemistry, 2017
It is difficult to identify reliable reference genes for transcriptomic analyses in biofluids such as saliva. This situation is particularly relevant for the newborn population, where rapid development is associated with dynamic changes in gene expression.
P, Khanna, K L, Johnson, J L, Maron
openaire   +2 more sources

High-Throughput RT-qPCR for the Analysis of Circulating MicroRNAs

2017
Reverse transcription followed by real-time or quantitative polymerase chain reaction (RT-qPCR) is the gold standard for validation of results from transcriptomic profiling studies such as microarray and RNA sequencing. The current need for most studies, especially biomarker studies, is to evaluate the expression levels or fold changes of many ...
Geok Wee, Tan, Lu Ping, Tan
openaire   +2 more sources

Whole-Genome RT-qPCR MicroRNA Expression Profiling

2011
MicroRNAs (miRNAs) are small noncoding RNA molecules that function as negative regulators of gene expression. They are essential components of virtually every biological process and deregulated miRNA expression has been reported in a multitude of human diseases including cancer.
Pieter, Mestdagh, Stefaan, Derveaux, Jo, Vandesompele   +2 more
openaire   +2 more sources

mRNA and microRNA quality control for RT-qPCR analysis

Methods, 2010
The importance of high quality sample material, i.e. non-degraded or fragmented RNA, for classical gene expression profiling is well documented. Hence, the analysis of RNA quality is a valuable tool in the preparation of methods like RT-qPCR and microarray analysis. For verification of RNA integrity, today the use of automated capillary electrophoresis
C, Becker, A, Hammerle-Fickinger, I, Riedmaier, M W, Pfaffl   +3 more
openaire   +2 more sources