Results 251 to 260 of about 495,188 (306)

Coordination of Cyanobacterial Nitrate Assimilation and Photosynthesis by a Novel PsbO‐Interacting Protein PirN

Advanced Science, EarlyView.
PirN was identified as a previously uncharacterized PsbO‐interacting protein specifically induced in nitrate‐grown cyanobacteria. Loss of PirN leads to coordinated downregulation of PsbO and the nitrate reductase NarB, causing decreased PSII activity and impaired growth under nitrate conditions. Complementation with either PsbO or NarB largely restores
Chengcheng Huang, Zhen Xiao, Haitao Ge, Gaojie Wang, Jinghui Dong, Yan Wang, Hang Yang, Xing Wang, Hui Gao, Zhongshu Wang, Huanling Yang, Yuanya Zhang, Xiahe Huang, Wu Xu, Weimin Ma, Wenqiang Yang, Yingchun Wang   +16 more
wiley   +1 more source

Systematic misclassification of sylvatic dengue virus 2 (DENV-2) infections as "undetermined serotype": implications in routine RT-qPCR surveillance. [PDF]

Trop Med Health
Dieng I.
europepmc   +1 more source

miRNA Stem-loop RT-qPCR Method

BIO-PROTOCOL
openaire   +1 more source

A Functionally Conserved Enhancer is Critical for PGC1A Expression and Thermogenesis in Brown Fat

Advanced Science, EarlyView.
An evolutionarily conserved enhancer controls PGC1A expression and thermogenic activation in brown adipose tissue. Integrative multi‐omics analyses identify two BAT‐specific enhancers, with PGC1A‐En1 functioning as the dominant regulatory element that maintains thermogenic capacity across species.
Duo Su, Haosen Yang, Xiaokai Li, Tingting Jiang, Siyuan Zhan, Tao Zhong, Jiazhong Guo, Yan Wang, Li Li, Hongping Zhang, Linjie Wang   +10 more
wiley   +1 more source

Development and evaluation of a duplex RT-qPCR assay for the detection and identification of Mayaro and chikungunya viruses. [PDF]

J Clin Microbiol
Wesselmann KM, Baronti C, Nougairède A, Thirion L, de Lamballerie X, Charrel R, Pezzi L.   +6 more
europepmc   +1 more source

Engineered Bacteria Factory Integrating Drug Delivery and Antibody Manufacture for Activating the STING Signal Pathway Mediated Tumor Immunotherapy

Advanced Science, EarlyView.
To address the limitations of protein‐based ICIs in applications, this study constructed an engineered bacterium, HRB, capable of expressing aCD47 in hypoxic TME. Through the Schiff base reaction, responsive liposomes (LC) loaded with the STING agonist cGAMP were connected to the surface of bacteria to form HRB@LC.
Peng‐Shuo Dong, Tian Yao, Yong Li, Zi‐Hui Yan, Xiao‐Ting Xie, Jia‐Hua Zou, Kai Cheng, Jin‐Xuan Fan, Yuan‐Di Zhao, Qiu‐Ran Xu   +9 more
wiley   +1 more source

Validation of H5 influenza virus subtyping RT-qPCR assay and low prevalence of H5 detection in 2024-2025 influenza virus season. [PDF]

J Clin Microbiol
Bacsik DJ, Mills MG, Monroe LD, Spring C, Perez-Osorio AC, Reed JC, Fang FC, Bourassa L, Roychoudhury P, Crawford KHD, Snekvik K, Greninger AL.   +11 more
europepmc   +1 more source

Delivery of Pleckstrin‐Homology Domains Suppresses PI3K/Akt Signaling and Breast Cancer Metastasis

Advanced Science, EarlyView.
Current therapies curb tumor growth but not metastasis. Obscurin, a giant metastasis suppressor lost in breast cancer, restrains PI3K/Akt signaling but is impractical to restore. We deploy a mini‐obscurin, comprising the obscurin‐PH‐domain, which sequesters PI3K‐p85, potently suppressing invasion and metastasis.
Matthew Eason, Anindya Sen, Suhan Cho, Se Jong Lee, Keyata Thompson, Md Musavvir Mahmud, Poornima Dubey, Talia Guardia, Anthony Kim, Stuart Martin, Nathan Wright, Konstantinos Konstantopoulos, Aikaterini Kontrogianni‐Konstantopoulos   +12 more
wiley   +1 more source

First Molecular Insights into the Presence of Canine Kobuvirus in Ecuadorian Dogs Through the Standardization of a Sensitive SYBR Green RT-qPCR Assay. [PDF]

Vet Sci
Sanchez-Castro C, Loor-Giler A, Santander-Parra S, Campos M, Mena-Pérez R, Prado-Chiriboga S, Nuñez L.   +6 more
europepmc   +1 more source

Comparative analysis of RT-qPCR, flow cytometry, and Di-4-ANEPPDHQ fluorescence for distinguishing macrophages phenotypes. [PDF]

Biochem Biophys Rep
Siniyeh AA, Alshaer W, Elzogheir N, Al-Holi M, Alqudah DA, Abuarqoub D, Kwiatek JM.   +6 more
europepmc   +1 more source