Results 261 to 270 of about 495,188 (306)

Development of an RT-qPCR and a Next-Generation Sequencing approach to assess viral shedding of NDV-based SARS-CoV-2 variant vaccines. [PDF]

Microbiol Spectr
Boza M, Abdeljawad A, Slamanig S, Lemus N, Lai TY, Shea W, Sun W, Palese P, González-Domínguez I.   +8 more
europepmc   +1 more source

Selection and validation of reference genes for RT-qPCR normalization in dormant cancer cells. [PDF]

Sci Rep
Iskhakova ER, Aleksandrova KV, Suvorova II.   +2 more
europepmc   +1 more source

Comparative Evaluation of Computational Methods for Validating Housekeeping Gene RT-qPCR Data in 3T3-L1 Cells. [PDF]

Biomedicines
Ivanova Z, Grigorova N, Petrova V, Vachkova E, Beev G.   +4 more
europepmc   +1 more source

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Detection of Viroids Using RT-qPCR

2021
Viroids are the smallest known infectious pathogens. They are nonprotein-encoding, single-stranded, circular, naked RNA molecules that can cause several diseases in economically important crops. With the advent of thermal cyclers incorporating fluorescent detection, reverse transcription coupled to the quantitative polymerase chain reaction (RT-qPCR ...
Subhas, Hajeri, Georgios, Vidalakis, Raymond K, Yokomi   +2 more
openaire   +2 more sources

Novel duplex RT‐qPCR for animal rabies surveillance

Transboundary and Emerging Diseases, 2022
Rabies is a lethal zoonosis affecting mammals worldwide. Diagnosis of rabies follows international standard protocols, primarily relying on direct immunofluorescence (DI) followed by mouse inoculation test (MIT). WHO recommends molecular biology techniques such as RT-qPCR for replacing MIT to diagnose rabies in animal samples. Recently, a real-time PCR
Guilherme Augusto Minozzo, Thaila Francini Corona, Elaine Cristina Rodrigues da Cruz, Wagner Antonio Chiba de Castro, Louise Bach Kmetiuk, Andrea Pires dos Santos, Alexander Welker Biondo, Irina Nastassja Riediger   +7 more
openaire   +2 more sources

Qualitätsmanagement in der RT-qPCR

BIOspektrum, 2012
Several aspects in the numerous steps of a reverse transcription (RT) quantitative PCR may interfere with the result’s validity. Therefore, before starting the proper investigation, a particular assay establishment is required.
Catrin Wernicke, Philipp Franke, Lars Radke, Stephan Berge, Marcus Frohme   +4 more
openaire   +1 more source

Whole-Genome RT-qPCR MicroRNA Expression Profiling

2011
MicroRNAs (miRNAs) are small noncoding RNA molecules that function as negative regulators of gene expression. They are essential components of virtually every biological process and deregulated miRNA expression has been reported in a multitude of human diseases including cancer.
Pieter, Mestdagh, Stefaan, Derveaux, Jo, Vandesompele   +2 more
openaire   +2 more sources