Results 61 to 70 of about 256,479 (299)
Directed evolution of enzymes at the crossroads of tradition and innovation
FEBS Open Bio, EarlyView.An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova +2 more
wiley +1 more source
Firewalls - Rules to Rule the Rules
, 2014 This paper was written in 2014 as part of a research project at scip AG, Switzerland. It was initially published online at https://www.scip.ch/en/?labs.20140403 and is available in English and German. Providing our clients with innovative research for the information technology of the future is an essential part of our company culture.
openaire +2 more sources
From rules to rule patterns [PDF]
, 1996 Rule-based systems are a commonly accepted solution for smoothly capturing the context-dependent and time-dependent organizational knowledge of large enterprises, also known as business policies. At the same time, however, the design of rule-based applications is one of the most pressing open research problems. This is largely because of the expressive
Gerti Kappel +3 more
openaire +2 more sources
Guiding AlphaFold to predict how Munc13‐1 opens Syntaxin‐1
FEBS Open Bio, EarlyView.The syntaxin‐1 Habc‐domain (orange), linker (pink) and SNARE motif (yellow) form a closed conformation that binds to Munc18‐1 (violet) and is opened by the Munc13‐1 MUN domain (cyan) to form the SNARE complex that triggers neurotransmitter release.
Madhurima Chattopadhyay +2 more
wiley +1 more source
Market forces in European soccer [PDF]
Recent decades have witnessed major changes in the market for European soccer. The most profound were the Bosman ruling, which lifted restrictions in the European labor market for soccer talent, and the introduction of the Champions’ League, a high ...
Koning, Ruud H. +2 more
core +2 more sources
Small RNA pathways in mammalian oocytes
FEBS Open Bio, EarlyView.Three distinct small RNA pathways operate in mammalian oocytes: RNAi interference (RNAi), the microRNA (miRNA) pathway, and the PIWI‐associated RNA (piRNA) pathway. These pathways use small RNAs to guide sequence‐specific repression and contribute to oocyte biology by targeting genes and mobile elements or appear insignificant since different ...
Petr Svoboda, Josef Pasulka
wiley +1 more source
FEBS Open Bio, EarlyView.The brown marmorated stink bug, Halyomorpha halys, mainly secretes 4‐oxo‐(E)‐2‐hexenal, (E)‐2‐decenal, dodecane and tridecane, a mixture representative of that released by many heteropterans. We demonstrated that H. halys can synthesise these secretory components de novo using dietary glucose.
Haruna Fujimori, Koji Noge
wiley +1 more source
“This is a British Colony”: The Ruling-Class Politics of the Seafarers' Strike, 1878–79
, 2013 The seafarers' strike of 1878–79 has occupied a special place in the history of White Australia, symbolising the idea that militant working class action led to the racial exclusion of Chinese and other non-white people. This article demonstrates that the
Phil Griffiths, Griffiths, Phil
core +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
FEBS Open Bio, EarlyView.An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Heterotropic regulation and negative homotropic cooperativity
FEBS Open Bio, EarlyView.We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea +5 more
wiley +1 more source