Results 161 to 170 of about 234,228 (200)
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2018
Plastic strain of glycerol and PEO-1500 frozen aqueous solutions has been studied and an alteration in their plastic characteristics when achieving certain (threshold) concentrations of cryoprotective substances has been established. This change is associated to local closed liquid phase inclusion transition into the through liquid interlayers, that ...
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Plastic strain of glycerol and PEO-1500 frozen aqueous solutions has been studied and an alteration in their plastic characteristics when achieving certain (threshold) concentrations of cryoprotective substances has been established. This change is associated to local closed liquid phase inclusion transition into the through liquid interlayers, that ...
+8 more sources
Electrotransformation of Saccharomyces cerevisiae
2019Intact yeast cell transformation is easily achieved by gene electrotransfer (GET). The procedure is fast and efficient in terms of transformants/μg DNA. Yeast cells in exponential growth phase are washed, treated for a short period with dithiothreitol (DTT) and then mixed with the plasmid DNA in a buffer with a low conductivity.
Meilhoc, Eliane, Teissie, Justin
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Saccharomyces cerevisiae Hsp60
1997Abstract The HSP60 gene of Saccharomyces cerevisiae was identified in the conditional mutant mif4 that lacked enzymatic activity of imported ornithine transcarbamylase (Cheng et al., 1989). Concurrently, the yeast HSP60 gene was isolated by screening a A-gt11 genomic library with a polyclonal antiserum raised against purified Tetra ...
Langer, T., Neupert, W.
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Saccharomyces cerevisiae Hsp78
1997Abstract The HSP78 gene was isolated from a A-gt11 expression library (accession numbers: GenBank L16533; PIR S39001; SwissProt P33416). Monoclonal antibodies were raised against yeast mitochondrial ribosomal proteins, used for screening the library (Leonhardt etal., 1993).
Schmitt, M., Langer, T.
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Mutagenesis in Saccharomyces Cerevisiae
1982Publisher Summary This chapter discusses the mutagenesis in Saccharomyces cerevisiae and compares it with what is known about the same process in E. coli. It also discusses the experimental systems available for mutation research in bakers' yeast, the result of investigations concerning induced and spontaneous mutagenesis in nuclear and ...
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Saccharomyces Cerevisiae Growth Media
2013This protocol describes the preparation of different types of media to grow the yeast Saccharomyces cerevisiae, both on plates and in liquid culture.
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Flocculation in Saccharomyces Cerevisiae
2009Yeast flocculation is a reversible, non-sexual and multivalent process of cell aggregation into multicellular masses, called flocs, with the subsequent rapid removal of flocs from the medium in which they are suspended. Traditionally associated with beer production, flocculation might also be useful in modern biotechnology as a low cost and easy method
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2013
???????????????????? ?????????????????????????????????? ?????????????????????? ?????????????????? ???????????? S. cerevisi????, ???????????????????????????????? ?? ???????????????? ???10% ???????????????????????????????????? ??? 0,135?? NaCl ??? ???????????, ?????????? ?????????????????????????? ???? ???????????????????? 1, 5 ?? 10????/??????. ?????????
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???????????????????? ?????????????????????????????????? ?????????????????????? ?????????????????? ???????????? S. cerevisi????, ???????????????????????????????? ?? ???????????????? ???10% ???????????????????????????????????? ??? 0,135?? NaCl ??? ???????????, ?????????? ?????????????????????????? ???? ???????????????????? 1, 5 ?? 10????/??????. ?????????
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