Results 121 to 130 of about 155,571 (316)

Engineering Murine Cross‐Reactivity Into an Affibody to Human Death Receptor 5

open access: yesBiotechnology and Bioengineering, EarlyView.
ABSTRACT Interspecies cross‐reactive protein therapeutics that target conserved epitopes across species are critical for translational research. The present study showcases the engineering of an affibody molecule, originally discovered for binding to human death receptor 5 (hDR5) with 94 nM affinity, to simultaneously acquire cross‐reactivity to murine
Tse‐Han Kuo   +3 more
wiley   +1 more source

Cyclic AMP Receptor Protein from Yeast Mitochondria [PDF]

open access: yes, 1985
We have identified and characterized a cyclic AMP receptor protein in mitochondria of the yeast Saccharomyces cerevisiae. The binding is specific for cyclic nucleotides, particularly for cyclic AMP which is bound with high affinity (Kd of 10(-9) M) at 1 ...
Rödel, Gerhard   +2 more
core  

KNR4, a suppressor of Saccharomyces cerevisiae cwh mutants, is involved in the transcriptional control of chitin synthase genes

open access: yes, 1999
The KNR4 gene, originally isolated by complementation of a K9 killer-toxinresistant mutant displaying reduced levels of both 1,3-b-glucan and 1,3-bglucan synthase activity, was recloned from a YCp50 genomic library as a suppressor of Saccharomyces ...
Francois, Jean   +10 more
core   +1 more source

Functional analysis of the "Saccharomyces cerevisiae" Npr1 protein kinase [PDF]

open access: yes, 2007
The uptake and processing of nutrients is highly regulated. Cells adapt to changes of the availability of nutrients to provide a complete set of transporters and metabolizing enzymes for optimal use of the available nutrients.
Gander, Stefan
core   +1 more source

A Microtiter Plate Assay as a Reliable Method to Assure the Identification and Classification of the Veil-Forming Yeasts during Sherry Wines Ageing

open access: yesFermentation, 2017
Yeasts involved in veil formation during biological ageing of Sherry wines are mainly Saccharomyces cerevisiae, and they have traditionally been divided into four races or varieties: beticus, cheresiensis, montuliensis and rouxii.
Marina Ruíz-Muñoz   +5 more
doaj   +1 more source

A Modular Nucleoside Kinase Cascade for the Synthesis of Ribonucleoside Triphosphates

open access: yesBiotechnology and Bioengineering, EarlyView.
Damm et al. report standardized enzymatic cascades for production of all canonical nucleoside triphosphates from the corresponding nucleosides, using kinase‐catalyzed phosphorylation from acetyl phosphate. Reactions afforded ∼ 100 mM product in near‐quantitative conversion.
Oliver T. Damm   +3 more
wiley   +1 more source

Optimized protein extraction for quantitative proteomics of yeasts [PDF]

open access: yes, 2007
BACKGROUND: The absolute quantification of intracellular protein levels is technically demanding, but has recently become more prominent because novel approaches like systems biology and metabolic control analysis require knowledge of these parameters ...
von der Haar, Tobias   +1 more
core   +1 more source

What Makes an “Ideal” Cell Line for Recombinant Adeno‐Associated Virus Production?

open access: yesBiotechnology and Bioengineering, EarlyView.
Several host cell types have been used to produce rAAVs to date. Cell line‐specific traits that are beneficial in the context of rAAV manufacturing are reviewed here, with the goal of developing a consensus on the ‘ideal’ characteristics that an rAAV production host should possess (created with Biorender.com).
James Conheady   +6 more
wiley   +1 more source

Heterologous prion-forming proteins interact to cross-seed aggregation in Saccharomyces cerevisiae

open access: yesScientific Reports, 2017
The early stages of protein misfolding remain incompletely understood, as most mammalian proteinopathies are only detected after irreversible protein aggregates have formed.
Kathryn M. Keefer   +2 more
doaj   +1 more source

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