Engineering Saccharomyces cerevisiae for efficient production of recombinant proteins
Saccharomyces cerevisiae is an excellent microbial cell factory for producing valuable recombinant proteins because of its fast growth rate, robustness, biosafety, ease of operability via mature genomic modification technologies, and the presence of a ...
Shuo Yang +5 more
doaj +1 more source
Expansion of a Telomeric FLO/ALS-Like Sequence Gene Family in Saccharomycopsis fermentans
Non-Saccharomyces species have been recognized for their beneficial contribution to fermented food and beverages based on their volatile compound formation and their ability to ferment glucose into ethanol.
Beatrice Bernardi +2 more
doaj +1 more source
Histone Modification Complex JMJ704‐HDA709 Negatively Regulates Salinity Tolerance in Rice
This study reveals that the rice histone demethylase JMJ704 interacts with HDA709―a H3K9ac deacetylase characterized herein―to form a chromatin‐modifying complex. Under salt stress, OsWRKY72 recruits this complex through interaction with JMJ704 to target loci, repressing the expression of oxidative stress and salt‐responsive genes via removal of ...
Jing Wang +9 more
wiley +1 more source
Malic acid is a potential biomass-derivable "building block" for chemical synthesis. Since wild-type Saccharomyces cerevisiae strains produce only low levels of malate, metabolic engineering is required to achieve efficient malate production with this ...
Van Maris, A.J.A. (author) +23 more
core +1 more source
The isolation and characterisation of temperature-dependent Ricin A chain molecules in Saccharomyces cerevisiae. [PDF]
Ricin is a heterodimeric plant protein that is potently toxic to mammalian cells. Toxicity results from the catalytic depurination of eukaryotic ribosomes by ricin A chain (RTA) that follows toxin endocytosis to, and translocation across, the ...
Ladds, Graham +15 more
core +1 more source
Summary Heterologous protein expression in yeast, mostly in Saccharomyces cerevisiae and Pichia pastoris, is a well‐established and widely used technique.
Mario González +3 more
doaj +1 more source
Synergistic HMGN1 and VP64 Fusions Potentiate High‐Precision and PAM‐Flexible Base Editing
A novel CDA1Δ‐SpRY architecture fused with HMGN1 and VP64 yields a nearly PAM‐less base editing platform. By focusing cytosine conversion predominantly at position −18, this synergistic complex ensures highly precise targeting. Demonstrating enhanced efficiency across diverse models, including yeast and rice, the platform offers a robust solution for ...
Xi Luo +11 more
wiley +1 more source
Asking the 5 W's for designing next‐generation bioprocessing
Abstract Biotechnology is expanding beyond traditional, centralized fermentation and toward next‐generation bioprocessing paradigms that emphasize flexible deployment outside the laboratory with application‐specific performance. However, many bioprocesses fail to translate beyond proof‐of‐concept into industrially viable systems because early design ...
Sangdo Yook +4 more
wiley +1 more source
Clonagem de transportadores de xilose de Scheffersomyces stipitis em Saccharomyces cerevisiae recombinante [PDF]
TCC(graduação) - Universidade Federal de Santa Catarina. Centro de Ciências Biológicas. Biologia.A tendência mundial em reduzir e substituir o uso de combustíveis fósseis por formas mais renováveis de energia intensificou a procura por alternativas mais ...
Scheid, Bruna
core
The essential endoplasmic reticulum chaperone Rot1 is required for protein N- and O-glycosylation in yeast [PDF]
Rot1 is an essential yeast protein originally shown to be implicated in such diverse processes such as β-1,6-glucan synthesis, actin cytoskeleton dynamics, or lysis of autophagic bodies.
Lehle, L. +8 more
core +1 more source

