Results 201 to 210 of about 139,200 (267)

Homeostatic Influence of Fig4 Outside of the Fab1‐Vac14‐Fig4 Complex in Saccharomyces cerevisiae

open access: yesMolecular Microbiology, EarlyView.
Disease‐related Fig4 mutants displaying impaired association with the Fab1‐Vac14‐Fig4 complex confer rapamycin tolerance in Saccharomyces cerevisiae independent of Vac14 and Fig4 catalytic function. Fig4 may thus modulate homeostasis through uncharacterized molecular interactions that lead to altered function when not bound to Vac14. ABSTRACT The lipid
Hannah E. Reeves   +13 more
wiley   +1 more source

Melatonin-protein interactions are dependent on yeast carbon metabolism. [PDF]

open access: yesSci Rep
Morcillo-Parra MA   +5 more
europepmc   +1 more source

Transcriptional gene fusions via targeted integration at safe harbors for high transgene expression in Chlamydomonas reinhardtii

open access: yesNew Phytologist, EarlyView.
Summary Conventional genetic engineering in green microalgae employs error‐prone nonhomologous end joining to integrate recombinant DNA at double‐strand breaks generated at random positions across the nuclear genome. This typically results in variable transcription strength and requires a labor‐intensive screening procedure to identify transformants ...
Nick Jacobebbinghaus   +5 more
wiley   +1 more source

The genetic basis of microbiome recruitment in grapevine and its association with fermentative and pathogenic taxa

open access: yesNew Phytologist, EarlyView.
Summary While grapevine is an exceptional perennial model for studying host‐microbiome interactions, the host genome's role in microbiome assembly is often masked by environmental factors. This research provides a first insight into the genetic mechanisms shaping berry‐associated microbial communities.
Lena Flörl   +7 more
wiley   +1 more source

The clarification of two hydroxylation steps in the conversion of cholesterol to OSW‐1 in Ornithogalum saundersiae

open access: yesPlant Biotechnology Journal, EarlyView.
Summary OSW‐1 is a cholestane saponin uniquely produced in Ornithogalum saundersiae and exhibits a potential antitumor activity. The enzymes responsible for OSW‐1 biosynthesis in O. saundersiae, however, remain unclear. Herein, cholesterol was confirmed to be the precursor of OSW‐1 using stable‐isotope labeling method.
Shu‐Yue Zhang, Jian‐Qiang Kong
wiley   +1 more source

Localising enzymes to biomolecular condensates increases their accumulation and benefits engineered metabolic pathway performance in Nicotiana benthamiana

open access: yesPlant Biotechnology Journal, EarlyView.
Summary The establishment of Nicotiana benthamiana as a robust biofactory is complicated by issues such as product toxicity and proteolytic degradation of target proteins/introduced enzymes. Here we investigate whether biomolecular condensates can be used to address these problems. We engineered biomolecular condensates in N.
Anya L. Lindström Battle   +3 more
wiley   +1 more source

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