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Engineering Yeast Extracellular Vesicle Biogenesis Through Rewiring Membrane Trafficking Pathways. [PDF]
Li Y +11 more
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Multifunctional Bioactivity of <i>Saccharomyces cerevisiae</i> Extracellular Vesicle in Hair Follicle-Related Cellular Models. [PDF]
Park HS, Shin E, Shin S.
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Purification of Membrane Proteins Overexpressed in Saccharomyces cerevisiae
2022Membrane protein (MP) functional and structural characterization requires large quantities of high-purity protein for downstream studies. Barriers to MP characterization include ample overexpression, solubilization, and purification of target proteins while maintaining native activity and structure.
Landon, Haslem +4 more
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Identification of CBS2 as a mitochondrial protein in Saccharomyces cerevisiae
Molecular and General Genetics MGG, 1990The nuclear genome encoded yeast protein CBS2 is required for translational activation of mitochondrial cytochrome b RNA. Genetic studies have shown that the target sequence of the CBS2 protein is the 5' untranslated leader sequence of cytochrome b RNA. Here we report on the intracellular localization of CBS2.
U, Michaelis, G, Rödel
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Protein arginine methylation in Saccharomyces cerevisiae
The FEBS Journal, 2012Recent research has implicated arginine methylation as a major regulator of cellular processes, including transcription, translation, nucleocytoplasmic transport, signalling, DNA repair, RNA processing and splicing. Arginine methylation is evolutionarily conserved, and it is now thought that it may rival other post‐translational modifications such as ...
Jason K K, Low, Marc R, Wilkins
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Adaptation of Saccharomyces cerevisiae expressing a heterologous protein
Journal of Biotechnology, 2008Production of the heterologous protein, bovine aprotinin, in Saccharomyces cerevisiae was shown to affect the metabolism of the host cell to various extent depending on the strain genotype. Strains with different genotypes, industrial and laboroatory, respectively, were investigated.
Astrid Mørkeberg, Krogh +5 more
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Saccharomyces Cerevisiae Strains that Overexpress Heterologous Proteins
Nature Biotechnology, 1991We describe a system that facilitates the selection of host mutants that overproduce a range of secreted and internally produced heterologous proteins in Saccharomyces cerevisiae. These mutants were initially selected for their ability to oversecrete recombinant human albumin (rHA), as detected by a direct visual assay that relies upon antibody ...
D, Sleep +7 more
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Coenzyme a-synthesizing protein complex of Saccharomyces cerevisiae
Molecular and Cellular Biochemistry, 1980The coenzyme A-synthesizing protein complex (CoA-SPC) is a multienzyme complex of Saccharomyces cerevisiae (Bakers' yeast), which has a molecular weight in excess of 200,000 as determined by Sephadex G-200 column chromatography. This multienzyme complex, which is insoluble in the crude yeast cell lysate, has been purified 229-fold. A cellular component
E T, Bucovaz +9 more
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