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Engineering Yeast Extracellular Vesicle Biogenesis Through Rewiring Membrane Trafficking Pathways. [PDF]

open access: yesMicrob Biotechnol
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Purification of Membrane Proteins Overexpressed in Saccharomyces cerevisiae

2022
Membrane protein (MP) functional and structural characterization requires large quantities of high-purity protein for downstream studies. Barriers to MP characterization include ample overexpression, solubilization, and purification of target proteins while maintaining native activity and structure.
Landon, Haslem   +4 more
openaire   +2 more sources

Identification of CBS2 as a mitochondrial protein in Saccharomyces cerevisiae

Molecular and General Genetics MGG, 1990
The nuclear genome encoded yeast protein CBS2 is required for translational activation of mitochondrial cytochrome b RNA. Genetic studies have shown that the target sequence of the CBS2 protein is the 5' untranslated leader sequence of cytochrome b RNA. Here we report on the intracellular localization of CBS2.
U, Michaelis, G, Rödel
openaire   +2 more sources

Protein arginine methylation in Saccharomyces cerevisiae

The FEBS Journal, 2012
Recent research has implicated arginine methylation as a major regulator of cellular processes, including transcription, translation, nucleocytoplasmic transport, signalling, DNA repair, RNA processing and splicing. Arginine methylation is evolutionarily conserved, and it is now thought that it may rival other post‐translational modifications such as ...
Jason K K, Low, Marc R, Wilkins
openaire   +2 more sources

Adaptation of Saccharomyces cerevisiae expressing a heterologous protein

Journal of Biotechnology, 2008
Production of the heterologous protein, bovine aprotinin, in Saccharomyces cerevisiae was shown to affect the metabolism of the host cell to various extent depending on the strain genotype. Strains with different genotypes, industrial and laboroatory, respectively, were investigated.
Astrid Mørkeberg, Krogh   +5 more
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Saccharomyces Cerevisiae Strains that Overexpress Heterologous Proteins

Nature Biotechnology, 1991
We describe a system that facilitates the selection of host mutants that overproduce a range of secreted and internally produced heterologous proteins in Saccharomyces cerevisiae. These mutants were initially selected for their ability to oversecrete recombinant human albumin (rHA), as detected by a direct visual assay that relies upon antibody ...
D, Sleep   +7 more
openaire   +2 more sources

Coenzyme a-synthesizing protein complex of Saccharomyces cerevisiae

Molecular and Cellular Biochemistry, 1980
The coenzyme A-synthesizing protein complex (CoA-SPC) is a multienzyme complex of Saccharomyces cerevisiae (Bakers' yeast), which has a molecular weight in excess of 200,000 as determined by Sephadex G-200 column chromatography. This multienzyme complex, which is insoluble in the crude yeast cell lysate, has been purified 229-fold. A cellular component
E T, Bucovaz   +9 more
openaire   +2 more sources

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