Results 271 to 280 of about 1,785,581 (311)
Some of the next articles are maybe not open access.
Detection of Protein Arginine Methylation in Saccharomyces cerevisiae
2014Protein arginine methylation has emerged to be an important regulator of cellular protein functions. Techniques that uncover the presence of methylarginines on a protein provide an important step towards understanding the functional role of arginine methylation.
Christopher A, Jackson, Michael C, Yu
openaire +2 more sources
Classical NLS Proteins from Saccharomyces cerevisiae
Journal of Molecular Biology, 2008Proteins can enter the nucleus through various receptor-mediated import pathways. One class of import cargos carries a classical nuclear localization signal (cNLS) containing a short cluster of basic residues. This pathway involves importin alpha (Impalpha), which possesses the cNLS binding site, and importin beta (Impbeta), which translocates the ...
Silvia, Hahn +3 more
openaire +2 more sources
Overview of Protein Expression in Saccharomyces cerevisiae
Current Protocols in Protein Science, 1995AbstractThis overview presents vectors and host strains that are available to direct gene expression in S. cerevisiae, including information on promoters, vector maintenance and copy number, transcription terminators, and selectable markers. Challenges to the expression of foreign proteins are also covered, including attainment of desired production ...
R L, Strausberg, S L, Strausberg
openaire +2 more sources
Evaluation of Unconventional Protein Secretion in Saccharomyces cerevisiae
2014Recent development of large-scale analyses such as the secretome analysis has enabled the discovery of a vast number of intracellular proteins that are secreted outside the cell. Often, these proteins do not contain any known signal sequence required for conventional protein secretion.
Natsuko, Miura, Mitsuyoshi, Ueda
openaire +2 more sources
Binding of Saccharomyces cerevisiae extracellular proteins to glucane
Archives of Biochemistry and Biophysics, 1992Interactions of Saccharomyces cerevisiae cell wall proteins with purified yeast glucane were studied. Using the beta-glucanase (BGL2 gene product) as the model cell wall protein, strong binding to glucane was demonstrated at pH lower than 7, while at pH higher than 8 the reaction did not occur.
V, Mrsa, T, Ugarković, S, Barbarić
openaire +2 more sources
Scarless Genomic Protein Labeling in Saccharomyces cerevisiae
2020Labeling a protein of interest is widely used to examine its quantity, modification, localization, and dynamics in the budding yeast Saccharomyces cerevisiae. Fluorescent proteins and epitope tags are often used as protein fusion tags to study target proteins.
Qian, Wang, Yu V, Fu, Wei, Xiao
openaire +2 more sources
High mobility group proteins of Saccharomyces cerevisiae
Biochemistry, 1980The yeast Saccharomyces cerevisiae contains four proteins having amino acid compositions typical of the high mobility group (HMG) proteins. Three of these are eluted from chromatin by 0.35 M NaCl; one is not, but it is eluted by 0.25 N HCl. It follows that HMGs cannot, in general, be defined by extractability criteria.
S, Weber, I, Isenberg
openaire +2 more sources
Identification of the Maltose Transport Protein of Saccharomyces cerevisiae
Biochemical and Biophysical Research Communications, 1994Identification of the maltose transport protein of Saccharomyces cerevisiae was attempted by searching for maltose-inducible proteins in isolated plasma membranes. Membranes from maltose-grown cells contained two proteins that were absent in glucose-grown cells.
P J, Van den Broek +6 more
openaire +2 more sources
RNA and protein elongation rates in Saccharomyces cerevisiae
Molecular and General Genetics MGG, 1973The RNA elongation rate has been measured in yeast by the kinetics of appearance of radioactivity in the different molecular weight classes by the method first developed by Bremer and Yuan (1968). Despite the limitations caused by the breakdown of the 35s rRNA precursor, an estimate of 29 to 38 nucleotides/second at 30° has been obtained for the RNA ...
openaire +2 more sources
Saccharomyces cerevisiae Ssb proteins
1997Abstract The SSB1, SSB2 genes (initially known as YG101, YG103) were isolated by virtue of their high degree of similarity to Drosophila HSP70 genes (Ingolia et al., 1982; Craig, Jacobsen, 1985).
T Ziegelhoffer, E A Craig
openaire +1 more source