Results 41 to 50 of about 137,997 (266)

Following the flux of long-chain bases through the sphingolipid pathway in vivo using mass spectrometry[S]

Journal of Lipid Research, 2016
Sphingolipids are essential components of the plasma membrane. Their synthesis is tightly controlled by regulatory proteins, which impinge on the rate-limiting step of the pathway, the condensation of serine and palmitoyl-CoA to long-chain base (LCB ...
Fernando Martínez-Montañés, Roger Schneiter   +1 more
doaj  

Characterising Maturation of GFP and mCherry of Genomically Integrated Fusions in Saccharomyces cerevisiae

Bio-Protocol, 2018
Single-molecule fluorescence microscopy enables unrivaled sub-cellular quantitation of genomically encoded fusions of native proteins with fluorescent protein reporters.
Sviatlana Shashkova, Adam Wollman, Stefan Hohmann, Mark C Leake   +3 more
doaj   +1 more source

Genome-wide analyses and functional classification of proline repeat-rich proteins: potential role of eIF5A in eukaryotic evolution. [PDF]

PLoS ONE, 2014
The eukaryotic translation factor, eIF5A has been recently reported as a sequence-specific elongation factor that facilitates peptide bond formation at consecutive prolines in Saccharomyces cerevisiae, as its ortholog elongation factor P (EF-P) does in ...
Ajeet Mandal, Swati Mandal, Myung Hee Park   +2 more
doaj   +1 more source

Tailoring a Functional Synthetic Microbial Community Alleviates Fusobacterium nucleatum‐infected Colorectal Cancer via Ecological Control

Advanced Science, EarlyView.
The bottom‐up strategy based on multiomics data is used for the SynCom design, and it successfully inhibited F. nucleatum growth and achieved stable colonization in vivo. In addition, it promoted tryptophan metabolism and secondary bile acid conversion, reduced lipid accumulation, relieved microbiome disorder, decreased inflammatory reaction, and ...
Zhongkun Zhou, Mengyue Yang, Hong Fang, Baizhuo Zhang, Yunhao Ma, Yongyuan Li, Yingjie Liu, Zeying Cheng, Yuanchun Zhao, Zhenzhen Si, Hongmei Zhu, Peng Chen   +11 more
wiley   +1 more source

Macromolecular protein crystallisation with biotemplate of live cells

Scientific Reports, 2022
Macromolecular protein crystallisation was one of the potential tools to accelerate the biomanufacturing of biopharmaceuticals. In this work, it was the first time to investigate the roles of biotemplates, Saccharomyces cerevisiae live cells, in the ...
Mubai Sun, Huaiyu Yang, Xinyu Miao, Weixian Wang, Jinghui Wang   +4 more
doaj   +1 more source

JA‐Mediated Regulation of Amino Acid Homeostasis Adjusts Metabolic Flux and Enhances Spider Mite Tolerance via the SlJAZ8‐SlWRKY57‐SlAVT6s Module in Tomato

Advanced Science, EarlyView.
This study elucidates a novel SlJAZ8‐SlWRKY57‐SlAVT6A/SlAVT6B module that effectively channels metabolic flux from primary to secondary metabolism, impacting plant growth, development, and resistance. Two novel vacuolar amino acid exporters, SlAVT6A and SlAVT6B, fine‐tunes trichomes, terpenes, and gibberellins, bolstering spider mite resistance.
Yingchen Hao, Xiaolong Wang, Langchen Guo, Lijun Xiang, Enxi Luo, Peng Cao, Penghui Liu, Yue Zhong, Chun Li, Jun Lai, Jun Yang, Shouchuang Wang   +11 more
wiley   +1 more source

Identification and Application of a Novel Patulin Degrading Enzyme From Meyerozyma guilliermondii

Advanced Science, EarlyView.
Patulin (PAT), a hazardous mycotoxin, threatens human health. A novel yeast‐derived short‐chain dehydrogenase/reductase effectively degrades PAT into nontoxic ascladiol via catalytic triad activity. The biological detoxification process has no significant effect on the quality of fresh pear juice.
Yu Zhang, Qianhua Zhao, Solairaj Dhanasekaran, Esa Abiso Godana, Yue Zhang, Xue Bai, Qiya Yang, Hongyin Zhang   +7 more
wiley   +1 more source

The SlDOF9‐SlSWEET17 Module: a Switch for Controlling Sugar Distribution Between Nematode Induced Galls and Roots in Tomato

Advanced Science, EarlyView.
Root‐knot nematode (RKN) disease seriously affects the yield and quality of vegetable crops. SlDOF9‐SlSWEET17 model helps plants resist RKN infection during early stage by switching off the sugar transport capacity of other SlSWEET proteins that are hijacked by RKNs.
Xiaoyun Wang, Zhimei Wang, Xinyue Tang, Jiamei Qin, Xiaoxuan Zhou, Lixia Gu, Huihui Bian, Lulu Sun, Huang Huang, Rui Yang, Jianli Wang, Shaohui Wang, Shuangchen Chen, Zhongren Yang, Wenchao Zhao   +14 more
wiley   +1 more source

Non-Saccharomyces in Winemaking: Source of Mannoproteins, Nitrogen, Enzymes, and Antimicrobial Compounds

Fermentation, 2020
Traditionally, non-Saccharomyces yeasts have been considered contaminants because of their high production of metabolites with negative connotations in wine.
Ricardo Vejarano
doaj   +1 more source

Optimisation of recombinant production of active human cardiac SERCA2a ATPase. [PDF]

PLoS ONE, 2013
Methods for recombinant production of eukaryotic membrane proteins, yielding sufficient quantity and quality of protein for structural biology, remain a challenge.
Ana V Antaloae, Cédric Montigny, Marc le Maire, Kimberly A Watson, Thomas L-M Sørensen   +4 more
doaj   +1 more source