Results 41 to 50 of about 139,200 (267)
Bio-Protocol, 2018 Single-molecule fluorescence microscopy enables unrivaled sub-cellular quantitation of genomically encoded fusions of native proteins with fluorescent protein reporters.
Sviatlana Shashkova +3 more
doaj +1 more source
Macromolecular protein crystallisation with biotemplate of live cells
Scientific Reports, 2022 Macromolecular protein crystallisation was one of the potential tools to accelerate the biomanufacturing of biopharmaceuticals. In this work, it was the first time to investigate the roles of biotemplates, Saccharomyces cerevisiae live cells, in the ...
Mubai Sun +4 more
doaj +1 more source
A Microfluidic Multiplex Sorter for Strain Development
Advanced Materials Technologies, Volume 10, Issue 6, March 18, 2025.A new multiplex method for high‐throughput screening of yeast strains based on glucoamylase production is presented. Droplets containing single mutant yeast cells are incubated for enzyme production. A sorting platform divides mutants by their high‐ and mid‐activity levels.
Chiara Leal‐Alves +6 more
wiley +1 more source
Genome-wide analyses and functional classification of proline repeat-rich proteins: potential role of eIF5A in eukaryotic evolution. [PDF]
PLoS ONE, 2014 The eukaryotic translation factor, eIF5A has been recently reported as a sequence-specific elongation factor that facilitates peptide bond formation at consecutive prolines in Saccharomyces cerevisiae, as its ortholog elongation factor P (EF-P) does in ...
Ajeet Mandal +2 more
doaj +1 more source
Advanced Synthesis &Catalysis, EarlyView.The halide motif is ubiquitous in organic synthesis, facilitating fine‐tuning of molecular properties and functionalization using substitution and cross‐coupling reactions. Combination of different flavin‐dependent tryptophan halogenases enables the installation of novel halogenation patterns on l‐tryptophan, which can be orthogonally addressed in ...
Nicolai Montua +4 more
wiley +1 more source
Advanced Sustainable Systems, EarlyView.The insect‐microbe interplay fulfills a key ecological role involving the degradation of ligninocellulose biomass. This is coordinated by the secretion of different kinds of proteins, of which ligninolytic enzymes, such as laccases (Lacs), are of prime importance.
Michael Dare Asemoloye +11 more
wiley +1 more source
Loss of NR2F6 Protects from Salmonella Typhimurium Infection
Advanced Science, EarlyView.Loss of nuclear receptor NR2F6 reduces tissue‐resident macrophage populations. Nr2f6‐deficient mice are protected from weight loss and bacterial load during infection with Salmonella Typhimurium. Pro‐inflammatory cytokines and iron levels are altered in infected Nr2f6‐deficient mice.
Johannes Woelk +8 more
wiley +1 more source
Fermentation, 2020 Traditionally, non-Saccharomyces yeasts have been considered contaminants because of their high production of metabolites with negative connotations in wine.
Ricardo Vejarano
doaj +1 more source
Optimisation of recombinant production of active human cardiac SERCA2a ATPase. [PDF]
PLoS ONE, 2013 Methods for recombinant production of eukaryotic membrane proteins, yielding sufficient quantity and quality of protein for structural biology, remain a challenge.
Ana V Antaloae +4 more
doaj +1 more source
Advanced Science, EarlyView.Current preclinical studies of AAV‐mediated gene therapy explore different strategies based on the characteristics of inner ear diseases. For genetic hearing loss, approaches include the replacement of a “good gene,” removal of a “bad gene,” or direct correction of mutations through base editing.
Fan Wu +7 more
wiley +1 more source