Results 131 to 140 of about 12,443 (164)
Promotion of spinosad biosynthesis by chromosomal integration of the Vitreoscilla hemoglobin gene in Saccharopolyspora spinosa [PDF]
Science China Life Sciences, 2012 To promote spinosad biosynthesis by improving the limited oxygen supply during high-density fermentation of Saccharopolyspora spinosa, the open reading frame of the Vitreoscilla hemoglobin gene was placed under the control of the promoter for the erythromycin resistance gene by splicing using overlapping extension PCR.
Xuezhi Ding, Liqiu Xia, Shengbiao Hu
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Applied Microbiology and Biotechnology, 2023
Pirin family proteins perform a variety of biological functions and widely exist in all living organisms. A few studies have shown that Pirin family proteins may be involved in the biosynthesis of antibiotics in actinomycetes. However, the function of Pirin-like proteins in S. spinosa is still unclear.
Li Cao +9 more
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Pirin family proteins perform a variety of biological functions and widely exist in all living organisms. A few studies have shown that Pirin family proteins may be involved in the biosynthesis of antibiotics in actinomycetes. However, the function of Pirin-like proteins in S. spinosa is still unclear.
Li Cao +9 more
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Selection of reference genes in Saccharopolyspora spinosa for real-time PCR
Transactions of Tianjin University, 2015Reverse transcription quantitative PCR(RT-qPCR)combined with the published genome information of Saccharopolyspora spinosa can allow sophisticated studies about S. spinosa, including studying the regulation of spinosyn biosynthesis, finding new target genes for engineering, and discovering and exploiting other macrolide secondary metabolites.
Xiaoqiang Jia, Chuanbo Zhang
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Proteomic insights into metabolic adaptation to deletion of metE in Saccharopolyspora spinosa
Applied Microbiology and Biotechnology, 2015Saccharopolyspora spinosa can produce spinosad as a major secondary metabolite, which is an environmentally friendly agent for insect control. Cobalamin-independent methionine synthase (MetE) is an important enzyme in methionine biosynthesis, and this enzyme is probably closely related to spinosad production.
Qi, Yang +8 more
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Transformation of Saccharopolyspora spinosa protoplasts with plasmid DNA modified in vitro to avoid host restriction [PDF]
Microbiology (United Kingdom), 1994 Summary: Saccharopolyspora spinosa protoplasts were not transformable by several different streptomycete plasmids, and S. spinosa was not a host for plaque formation by the Saccharopolyspora bacteriophages OSE60, OSE45, OSE57, OSE60, OSE69 or HP10. Extracts of S.
R H Baltz +2 more
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Antonie Van Leeuwenhoek, 2017
Spinosad, a universal bio-pesticide, is obtained from the soil actinomycete Saccharopolyspora spinosa. Dissolved oxygen, an important contributing factor in aerobic microbial fermentation, however, is not always available in sufficient amounts. To alleviate oxygen limitation in spinosad production, three different oxygen vectors, namely oleic acid ...
Xiaoqiang Jia, Chunzhe Lu
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Spinosad, a universal bio-pesticide, is obtained from the soil actinomycete Saccharopolyspora spinosa. Dissolved oxygen, an important contributing factor in aerobic microbial fermentation, however, is not always available in sufficient amounts. To alleviate oxygen limitation in spinosad production, three different oxygen vectors, namely oleic acid ...
Xiaoqiang Jia, Chunzhe Lu
exaly +3 more sources
Applied Microbiology and Biotechnology, 2015
Saccharopolyspora spinosa produces tetra-cyclic macrolide spinosyns, a group of highly efficient pesticidal agents. However, this species lacks efficient vectors for genetic manipulation. In this study, the circular plasmid pCM32 was newly isolated from Saccharopolyspora endophytica YIM 61095.
Jian, Chen +5 more
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Saccharopolyspora spinosa produces tetra-cyclic macrolide spinosyns, a group of highly efficient pesticidal agents. However, this species lacks efficient vectors for genetic manipulation. In this study, the circular plasmid pCM32 was newly isolated from Saccharopolyspora endophytica YIM 61095.
Jian, Chen +5 more
openaire +2 more sources
Effects of Glucose and Phosphate on Spinosad Fermentation by Saccharopolyspora spinosa
Chinese Journal of Chemical Engineering, 2006Abstract The effects of glucose and inorganic phosphate on mycelium growth and spinosad production with Saccharopolyspora spinosa were studied. The results showed that the increase of glucose concentration from 18.6g·L −1 to 58.8g·L −1 could promote the mycelium growth and spinosad production.
Zhihua JIN, Xiu CHENG, Peilin CEN
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Stepwise increase of spinosad production in Saccharopolyspora spinosa by metabolic engineering
Biochemical Engineering Journal, 2013Abstract Rational metabolic and cellular engineering approaches are useful in improving strain performance. In the last years, several studies not only clarified the biosynthetic pathway of spinosad, but also provided useful information of metabolic restrictions in the spinosyn biosynthetic pathway.
Chaoyou Xue +3 more
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Biotechnology Letters
The macrolide antibiotics spinosad, synthesized by Saccharopolyspora spinosa, is a highly effective, environmentally-friendly insecticide. However, due to poor fermentation performance and difficulties in engineering S. spinosa strains, the production cost of spinosad is still high, which restricts its industrial application.
Wenyu Lu
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The macrolide antibiotics spinosad, synthesized by Saccharopolyspora spinosa, is a highly effective, environmentally-friendly insecticide. However, due to poor fermentation performance and difficulties in engineering S. spinosa strains, the production cost of spinosad is still high, which restricts its industrial application.
Wenyu Lu
exaly +3 more sources