Results 91 to 100 of about 7,654,949 (307)

Structures of mycobacterial 3‐methylcrotonyl‐CoA carboxylase reveal carrier‐domain translocation between catalytic sites

open access: yesFEBS Letters, EarlyView.
Mycobacterial 3‐methylcrotonyl‐CoA carboxylase uses a mobile biotin‐carrying domain to shuttle a carboxyl group between two catalytic sites, enabling carboxylation of 3‐methylcrotonyl‐CoA during leucine breakdown. Cryo‐electron microscopy captures the carrier at both sites and reveals an inward loop movement that may prevent futile rebinding to the ...
Ajit Yadav   +2 more
wiley   +1 more source

Mitigating the Effect of Surfactants in Sample Preparation of a Phospholipid Formulation for Impurities Analysis by Reversed-Phase Liquid Chromatography

open access: yes, 2017
A surfactant-based diluent was used in sample preparation for reversed phase high performance liquid chromatography (HPLC) analysis of a drug product and its impurities in a phospholipid formulation.
Blasko, Andrei, Tam, Yat Kong
core  

The Shewanella oneidensis Fic enzyme SoFic targets the switch‐I region of EF‐Tu for AMPylation

open access: yesFEBS Letters, EarlyView.
Fic enzymes mediate diverse post‐translational modifications across all domains of life, including AMPylation. Prokaryotic EF‐Tu can be AMPylated and deAMPylated by the conserved Fic enzyme SoFic. Structural and biochemical approaches were used to characterize the effect of AMPylation on EF‐Tu, SoFic's enzymatic activities, and the enzyme‐target ...
Svenja Runge   +6 more
wiley   +1 more source

New trends in bioanalysis sampling and pretreatment: How modern microsampling is revolutionising the field

open access: yesAdvances in Sample Preparation
Microsampling technologies are revolutionising bioanalysis by enabling minimally invasive, decentralized sample collection, offering significant advantages over traditional methods in terms of patient compliance, cost-effectiveness, and analytical ...
Michele Protti   +4 more
doaj   +1 more source

Microextraction methods in food sample preparation

open access: yes, 2016
The new trends in food analysis are microextraction techniques due to their potential as fast, simple and inexpensive sample preparation, high enrichment factor, adaptability to field sampling and possibility of automation.
Damascelli A
core  

Chiral separation of chloroalkanes with the chromatographic column onboard Martian rovers

open access: yesFEBS Letters, EarlyView.
Computer image of the Rosalind Franklin Rover of ESA's ExoMars mission. ExoMars is scheduled to land on planet Mars in Oxia Planum in 2029. This area represents an interesting spot to look for biosignatures. Investigations of ExoMars include measurement on molecular chirality. We show that chiral chloroalkanes, that have been identified on Mars, can be
Asma Merzougui   +4 more
wiley   +1 more source

Application of deep eutectic solvents for the determination of inorganic analytes

open access: yesAdvances in Sample Preparation
Deep eutectic solvents (DESs), due to their unique physicochemical properties, such as low volatility, tunable viscosity, and excellent solvation capabilities, have emerged as promising alternatives to conventional solvents in the determination of ...
Vasil Andruch   +5 more
doaj   +1 more source

L‐aspartate oxidase provides new insights into fumarate reduction in anaerobic darkness in Synechocystis sp. PCC6803

open access: yesFEBS Letters, EarlyView.
Synechocystis strains deficient in succinate dehydrogenase (SDH) secrete more succinate than the WT under dark anaerobic conditions, supporting that SDH then primarily acts as SDH, not as a fumarate reductase. L‐aspartate oxidase (Laspo) from Synechocystis is functional under anaerobic conditions, reducing fumarate to succinate.
Kateryna Kukil   +3 more
wiley   +1 more source

Sustainable analytical approaches for microplastics in wastewater, sludge, and landfills: Challenges, fate, and green chemistry perspectives

open access: yesAdvances in Sample Preparation
The effects of microplastics (MPs) in wastewater, sludge, and landfills are analyzed and discussed, focusing on their pathways into the environment. The application of sludge as a soil amendment and the reuse of treated wastewater in agriculture have ...
Maryam Mallek, Damia Barcelo
doaj   +1 more source

Green analytical chemistry in sample preparation

open access: yes
Green chemistry principles have been integrated into various branches of chemistry, including analytical chemistry, in response to growing concerns about the ecosystem health and the adverse environmental impacts associated with analytical procedures ...
Abellán Martin, Sergio J.   +5 more
core   +1 more source

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