Results 271 to 280 of about 15,581,970 (330)

Efficient DNP NMR of membrane proteins: sample preparation protocols, sensitivity, and radical location

, 2016
Shu Y. Liao, Myungwoon Lee, Tuo Wang, Ivan V. Sergeyev, Mei Hong   +4 more
openalex   +1 more source

A general model for analysis of linear and hyperbolic enzyme inhibition mechanisms

FEBS Open Bio, EarlyView.
We developed a general enzyme kinetic model that integrates these six basic inhibition mechanism onto a single one. From this model, we deduced a general enzyme kinetic equation that through modulation of simple parameters, γ (the relative inhibitor affinity for two binding sites) and β (the reactivity of the enzyme–substrate–inhibitor complex), is ...
Rafael S. Chagas, Sandro R. Marana
wiley   +1 more source

Point-of-Care Extraction-Free Sample Preparation for Recombinase Polymerase Amplification of Nucleic Acid Targets in Buccal Swabs or Blood. [PDF]

ACS Omega
Wilkinson AF, Barra M, Richards-Kortum RR.   +2 more
europepmc   +1 more source

Thrombolytic proteins profiling: High‐throughput activity, selectivity, and resistance assays

FEBS Open Bio, EarlyView.
We present optimized biochemical protocols for evaluating thrombolytic proteins, enabling rapid and robust screening of enzymatic activity, inhibition resistance, and fibrin affinity, stimulation, and selectivity. The outcome translates to key clinical indicators such as biological half‐life and bleeding risk. These assays streamline the development of
Martin Toul, Alan Strunga, Jiri Damborsky, Zbynek Prokop   +3 more
wiley   +1 more source

Mitochondrial respiratory function in human platelets: Influence of sample preparation, assay buffer, and instrumental platform. [PDF]

Physiol Rep
Porter C, Treas L, Pavliv O, Sadler D, Cotter M, Rose S, Børsheim E, Diaz Fuentes EC.   +7 more
europepmc   +1 more source

Overview of molecular signatures of senescence and associated resources: pros and cons

FEBS Open Bio, EarlyView.
Cells can enter a stress response state termed cellular senescence that is involved in various diseases and aging. Detecting these cells is challenging due to the lack of universal biomarkers. This review presents the current state of senescence identification, from biomarkers to molecular signatures, compares tools and approaches, and highlights ...
Orestis A. Ntintas, Sylvia Vagena, Pavlos Pantelis, Giorgos Theocharous, Russel Petty, Konstantinos Evangelou, Vassilis G. Gorgoulis   +6 more
wiley   +1 more source

A Flexible End-to-End Automated Sample Preparation Workflow Enables Standardized and Scalable Bottom-up Proteomics. [PDF]

Anal Chem
Schär S, Räss L, Malinovska L, Savickas S, Cavallo F, Below C, Tognetti M, Shichkova P, Gourdet B, Robles G, Iu L, Vowinckel J, Feng Y, Hjerpe R, Bruderer R, Reiter L.   +15 more
europepmc   +1 more source

Long‐term culture of skin biopsies: maintenance of fibroblast production and competency of reprogramming

FEBS Open Bio, EarlyView.
Skin biopsies taken from a patient with an ultra‐rare disorder as well as controls were cultured for up to 473 days. The chunks of skin were serially transferred to a new culture plate when confluent with fibroblasts. Different generations of fibroblasts were analyzed for cell and molecular properties, proliferation, and competence for reprogramming to
Sudiksha Rathan‐Kumar, Michael A. Ripperger, Grant M. Westlake, Kevin C. Ess   +3 more
wiley   +1 more source

Impact of Sample Preparation Strategies on the Quantitative Accuracy of Low-Abundance Serum Proteins in Shotgun Proteomics. [PDF]

J Proteome Res
Katsavelis D, van der Hart MGC, Wolters JC, Permentier HP, Horvatovich P, Cremers TIFH.   +5 more
europepmc   +1 more source