Results 61 to 70 of about 13,319,037 (325)

Enteropathogenic E. coli shows delayed attachment and host response in human jejunum organoid‐derived monolayers compared to HeLa cells

FEBS Letters, EarlyView.
Enteropathogenic E. coli (EPEC) infects the human intestinal epithelium, resulting in severe illness and diarrhoea. In this study, we compared the infection of cancer‐derived cell lines with human organoid‐derived models of the small intestine. We observed a delayed in attachment, inflammation and cell death on primary cells, indicating that host ...
Mastura Neyazi, Pilar Samperio Ventayol, Natalie Burkard, Nicolas Schlegel, Carmen Aguilar, Sina Bartfeld   +5 more
wiley   +1 more source

Microextraction by packed sorbent: Introducing a novel hybrid silica-based chitosan-graphene oxide biosorbent for the evaluation of pesticides and antibiotics in food matrices

Advances in Sample Preparation
This study introduces a novel silica-graphene oxide@chitosan (SiGO@CS) material as a packed biosorbent for microextraction by packed sorbent (MEPS), followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis of pesticides (atrazine ...
Rafael O. Martins, Fernando M. Lanças
doaj   +1 more source

Green Approaches to Sample Preparation Based on Extraction Techniques

Molecules, 2020
Preparing a sample for analysis is a crucial step of many analytical procedures. The goal of sample preparation is to provide a representative, homogenous sample that is free of interferences and compatible with the intended analytical method.
A. A. Aly, T. Górecki
semanticscholar   +1 more source

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source

A miniaturized QuEChERS-DLLME method for simultaneous determination of acetamide, acrylamide, and glycidamide in infant formula using GC–MS

Advances in Sample Preparation
Infant formula is the only food product suitable for infants during the first months of life. Therefore, evaluating food contaminants is essential for ensuring infant food safety.
J.A. Custodio-Mendoza, M.P. España Fariñas, A.M. Ares-Fuentes, M.A. Kurek, A.M. Carro Díaz   +4 more
doaj   +1 more source

The anti‐CRISPR protein AcrIE8.1 inhibits the type I‐E CRISPR‐Cas system by directly binding to the Cascade subunit Cas11

FEBS Letters, EarlyView.
In this study, we present the structure of AcrIE8.1, a previously uncharacterized anti‐CRISPR protein that inhibits the type I‐E CRISPR‐Cas system. Through a combination of structural and biochemical analyses, we demonstrate that AcrIE8.1 directly binds to the Cas11 subunit of the Cascade complex to inhibit the CRISPR‐Cas system.
Young Woo Kang, Hyun Ho Park
wiley   +1 more source

One step derivatization and switchable hydrophilicity solvent-based microextraction for the determination of adamantane analogues in human urine by HPLC-FLD

Advances in Sample Preparation
The present study describes an “one-step” derivatization and microextraction using a pH-switchable hydrophilicity solvent for the determination of amantadine and memantine in human urine by liquid chromatography and fluorescence detection.
Marianna Ntorkou, Paraskevas D. Tzanavaras, Constantinos K. Zacharis   +2 more
doaj   +1 more source

Analytical and Sample Preparation Techniques for the Determination of Food Colorants in Food Matrices

Foods, 2020
Color additives are widely used by the food industry to enhance the appearance, as well as the nutritional properties of a food product. However, some of these substances may pose a potential risk to human health, especially if they are consumed ...
Konstantina Ntrallou, H. Gika, E. Tsochatzis   +2 more
semanticscholar   +1 more source

By dawn or dusk—how circadian timing rewrites bacterial infection outcomes

FEBS Letters, EarlyView.
The circadian clock shapes immune function, yet its influence on infection outcomes is only beginning to be understood. This review highlights how circadian timing alters host responses to the bacterial pathogens Salmonella enterica, Listeria monocytogenes, and Streptococcus pneumoniae revealing that the effectiveness of immune defense depends not only
Devons Mo, Catherine S. Palmer, Jacqueline M. Kimmey   +2 more
wiley   +1 more source

Determination of acylcarnitines in intact brain tumors using coated blade spray mass spectrometry (CBS-MS)

Advances in Sample Preparation
Potential biomarkers of cancerous alterations could be selected by profiling a wide range of analytes. For this purpose, analytical platforms enabling detailed analysis of different biological matrices are applied.
Joanna Bogusiewicz, Karol Jaroch, Jacek Furtak, Marcin Birski, Krystyna Soszyńska, Anna Majdańska, Agata Ryfa, Marek Harat, Barbara Bojko   +8 more
doaj   +1 more source