Results 61 to 70 of about 2,377,512 (309)

Greener solvents in extraction of proteins and peptides

Advances in Sample Preparation
The sample pretreatment still represents a fundamental aspect of any analytical workflow, and the implementation of sustainable and efficient extraction of proteins is essential to satisfy the increasing requirements for high-quality extraction of ...
Katarína Maráková
doaj   +1 more source

Preparation of 2D Crystals of Membrane Proteins for High-Resolution Electron Crystallography Data Collection [PDF]

Electron crystallography is a powerful technique for the structure determination of membrane proteins as well as soluble proteins. Sample preparation for 2D membrane protein crystals is a crucial step, as proteins have to be prepared for electron ...
Stahlberg, Henning, Abeyrathne, Priyanka D., Goldie, Kenneth N., Pantelic, Radosav S., Chami, Mohamed   +4 more
core   +1 more source

Recent developments in solid-phase microextraction for on-site sampling and sample preparation [PDF]

, 2011
Recent developments in solid-phase microextraction for on-site sampling and sample preparationOn-site sampling and sample preparation favor portable, solventless or even solvent-free techniques.
Duan, Chunfeng, Shen, Zheng, Wu, Dapeng, Guan, Yafeng, 关亚风   +4 more
core   +1 more source

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source

Microextraction by packed sorbent: Introducing a novel hybrid silica-based chitosan-graphene oxide biosorbent for the evaluation of pesticides and antibiotics in food matrices

Advances in Sample Preparation
This study introduces a novel silica-graphene oxide@chitosan (SiGO@CS) material as a packed biosorbent for microextraction by packed sorbent (MEPS), followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis of pesticides (atrazine ...
Rafael O. Martins, Fernando M. Lanças
doaj   +1 more source

Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation

FEBS Letters, EarlyView.
Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe, Tanmay Kumar Mohanty, Rashna Bhandari, Dorothea Fiedler   +3 more
wiley   +1 more source

A miniaturized QuEChERS-DLLME method for simultaneous determination of acetamide, acrylamide, and glycidamide in infant formula using GC–MS

Advances in Sample Preparation
Infant formula is the only food product suitable for infants during the first months of life. Therefore, evaluating food contaminants is essential for ensuring infant food safety.
J.A. Custodio-Mendoza, M.P. España Fariñas, A.M. Ares-Fuentes, M.A. Kurek, A.M. Carro Díaz   +4 more
doaj   +1 more source

Mitigating the Effect of Surfactants in Sample Preparation of a Phospholipid Formulation for Impurities Analysis by Reversed-Phase Liquid Chromatography [PDF]

, 2017
A surfactant-based diluent was used in sample preparation for reversed phase high performance liquid chromatography (HPLC) analysis of a drug product and its impurities in a phospholipid formulation.
Blasko, Andrei, Tam, Yat Kong
core  

The (Glg)ABCs of cyanobacteria: modelling of glycogen synthesis and functional divergence of glycogen synthases in Synechocystis sp. PCC 6803

FEBS Letters, EarlyView.
We reconstituted Synechocystis glycogen synthesis in vitro from purified enzymes and showed that two GlgA isoenzymes produce glycogen with different architectures: GlgA1 yields denser, highly branched glycogen, whereas GlgA2 synthesizes longer, less‐branched chains.
Kenric Lee, Dimitrios Bekiari, Sofia Doello, Karl Forchhammer   +3 more
wiley   +1 more source

One step derivatization and switchable hydrophilicity solvent-based microextraction for the determination of adamantane analogues in human urine by HPLC-FLD

Advances in Sample Preparation
The present study describes an “one-step” derivatization and microextraction using a pH-switchable hydrophilicity solvent for the determination of amantadine and memantine in human urine by liquid chromatography and fluorescence detection.
Marianna Ntorkou, Paraskevas D. Tzanavaras, Constantinos K. Zacharis   +2 more
doaj   +1 more source