Results 141 to 150 of about 224,524 (254)

A global phylogenetic framework for Diospyros L. (Ebenaceae, Ericales): enhanced taxon and sample coverage through NGS, Sanger sequencing and herbariomics. [PDF]

Ann Bot
Meeprom N, Linan A, Duangjai S, Puglisi C, Könyves K, Utteridge T, Culham A.   +6 more
europepmc   +1 more source

Sanger sequencing is a robust technique with few sequencing errors

Gene sequencing is a common technique used in a variety of biological research workflows. Currently, the gold standard method remains Sanger sequencing due to its reliability and low error rate. This work reports a small retrospective analysis of past Sanger sequencing data to assess the extent of low error rate that is inherent in the Sanger ...
openaire   +1 more source

PDHA1 Hyperactivation‐Mediated Glucose Metabolism Reprogramming Fuels Neutrophil NETosis and Promotes Acute Lung Injury

Advanced Science, EarlyView.
During NETosis, neutrophils shift metabolically toward enhanced OXPHOS and partially reduced glycolysis. This shift is driven by PDHA1 hyperactivity‐dependent selective metabolic reprogramming. The neutrophil‐targeted nanoparticle 3‑FP‑BSA NPs effectively inhibit NETosis and attenuate ALI.
Yu‐Biao Liu, Wen‐Jing Zhong, Chen‐Yu Zhang, Meng‐Rui Chen, Nan‐Shi‐Yu Yang, Han‐Xi Sha, Yan‐Ling Qiu, Hui Chen, Jin‐Tong Yang, Li‐Ying Liang, Jian‐Bing Xiong, Shao‐Kun Liu, Cha‐Xiang Guan, Jia‐Xi Duan, Yong Zhou   +14 more
wiley   +1 more source

Diagnostic accuracy of the droplet digital PCR POLE mutation test in endometrial cancer: comparison with Sanger sequencing and NGS. [PDF]

J Gynecol Oncol
Ouh YT, Cho HW, Min KJ, Min KJ, Lee JK, Chun Y, Hong JH.   +6 more
europepmc   +1 more source

A Fluorogenic Cysteine‐Tetrapeptide Tagging Strategy for Nonperturbative Protein Labeling and Protein–Ligand Interaction Tracking

Advanced Science, EarlyView.
A compact Phe‐Cys‐Pro‐Phe (FCPF) tag and an environment‐sensitive SBD‐Cl‐derived probe form a sequence‐selective fluorogenic pair for wash‐free live‐cell protein imaging. This minimally perturbing chemistry allows flexible insertion at termini or loops, produces strong fluorescence upon local microenvironment confinement, supports FRET‐assisted ...
Xiao Wang, Xingcan Pan, Yongling Ying, Ziqian Bai, Jingming Zhang, Zhuang Shao, Jialiang Qian, Hongwei Li, Ning Zhang, Fan Yang, Yuan Liu, Xing Yang   +11 more
wiley   +1 more source

SAGPEK: fast and flexible approach to identify genotypes of Sanger sequencing data. [PDF]

BMC Bioinformatics
Wang J, Sun S, Zhang Y, Huang N, Yang C, Gao Y, Wang X, Ju Z, Jiang Q, Xiao Y, Wei X, Liu W, Huang J.   +12 more
europepmc   +1 more source

Characterisation of human astrovirus in a diarrhoea outbreak using nanopore and Sanger sequencing protocols. [PDF]

Virol J, 2023
Li J, Yang L, Wang K, Gao Z, Li P, Lin Y, Jia L, Wang Q, Song H, Li P.   +9 more
europepmc   +1 more source

Assembling Sanger and Non-Sanger Sequencing Data [PDF]

, 2005
Platt, Darren, Richardson, Paul
openaire  

NSUN3‐Associated m5C Modification of tRF5‐GlyTCC Suppresses Colorectal Cancer Progression via RPL23‐Linked Nucleolar Stress and p53‐Associated Autophagy and Enhances 5‐FU Sensitivity

Advanced Science, EarlyView.
This study identifies that NSUN3‐mediated m5C modification stabilizes tRF5‐GlyTCC, linking ribosomal homeostasis, nucleolar stress responses, and p53 signaling changes. This regulatory axis suppresses colorectal cancer and enhances chemotherapy response to 5‐FU, positioning tRF5‐GlyTCC as a potential therapeutic target for improving drug sensitivity in
Xinhui Lv, Can Tang, Feiyang Song, Yu Wu, Yafang Chen, Sicong Liu, Ziwen Yuan, Guihua Wang, Xudong Wang   +8 more
wiley   +1 more source

Sanger sequencing of barcoded beads v1

This protocol is used to test the purity of barcoded hydrogel beads like: https://www.protocols.io/view/hydrop-v2-bead-generation-amp-ligation-barcoding-8epv5n11dv1b ...
openaire   +1 more source