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Sanger and Next-Generation Sequencing of AAT
2023Sequencing of DNA is normally the final procedure carried out to determine the actual pathogenic variants when the techniques used for genotyping are unable to provide complete identification of both AAT alleles. Gene sequencing of complete SERPINA1 gene by using the Sanger method or next-generation sequencing (NGS) is crucial to enable correct ...
Barzon V., Ferrarotti I., Ottaviani S.
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Sanger Sequencing of Xeno‐Nucleic Acid
Angewandte ChemieAbstract Xeno‐nucleic acids (XNAs) offer biostable genetic polymers for biotechnology and information technology, but are incompatible with prevailing DNA sequencing methodologies. Here we report a Sanger sequencing approach tailored for XNA, enabling direct readout of XNA strands up to 50 bases.
Yueyao Wang +12 more
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The Chromosome 6 Sequencing Project at the Sanger Centre
DNA Sequence, 1997Chromosome 6 is probably best known for encoding the major histocompatibility complex (MHC) which is essential to the human immune response. In addition, it has been shown to be associated with many diseases such Schizophrenia, Diabetes, Arthritis, Haemochromatosis, Narcolepsy, Epilepsy, Retinitis Pigmentosa, Deafness, Ovarian Cancer, and many more ...
T, Avis +13 more
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Evaluation of whole exome sequencing by targeted gene sequencing and Sanger sequencing
Clinica Chimica Acta, 2017Targeted gene sequencing (TGS) and whole exome sequencing (WES) are being used in clinical testing in laboratories. We compared the performances of TGS and WES using the same DNA samples.DNA was extracted from 10 endometrial tumor tissue specimens. Sequencing were performed with an Illumina HiSeq 2000.
Ya-Sian, Chang +3 more
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Targeted Amplicon Genotyping by Sanger Sequencing
This chapter presents a protocol for Sanger sequencing, a conventional yet rapid, cost-effective, and highly accurate method for targeted sequencing. It involves designing specific primers for forward and reverse template extension reactions, during which fluorophore labeled, chain terminating dideoxynucleotides are incorporated into newly replicated ...Anthony Torres, Reginald Gaudino
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Sanger Sequencing to Determine the Full-Length Sequence of Circular RNAs
The pre-existing theory of pre-mRNA splicing into linear mature RNA was questioned with the introduction of circular RNAs (circRNAs). Hundreds of studies using high throughput RNA-sequencing (RNA-seq) techniques and novel computational programs reported the abundant and ubiquitous expression of circRNAs originating by pre-mRNA backsplicing.Suman, Singh +2 more
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Low-Cost-Medium Throughput Sanger Dideoxy Sequencing
2008Serial analysis of gene expression (SAGE) requires the sequencing of DNA. The principal cost of SAGE is largely determined by the cost of sequencing. Therefore, it is important to have access to a robust and affordable sequencing system. Here, we describe such a system based on the sequencing of amplified inserts of concatemer-containing clones.
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Performance Comparison: Exome Sequencing as a Single Test Replacing Sanger Sequencing
Molecular Genetics and Genomics, 2021Hila Fridman +2 more
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Chapter Sixteen - Sanger Sequencing of Lyssaviruses
2014Sanger dideoxy terminator sequencing was developed by Fred Sanger and colleagues in 1977 and became the most widely used method for virus characterization in the last three decades. More recently, Sanger sequencing has been supplanted by Next-Generation Sequencing (NGS) methods, for the determination of viral genomes.
McElhinney, Lorraine M. +5 more
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