Results 61 to 70 of about 58,964 (242)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Femtosecond timing jitter from soliton fiber lasers mode-locked by carbon nanotube saturable absorbers

, 2012
We demonstrate sub-10-fs level timing jitter [integrated from 10 kHz to 40 MHz offset frequency] from 80-MHz soliton Er-fiber lasers mode-locked by carbon nanotube (CNT)-based saturable ...
Chur Kim, Kim, Chur, Kieu, Khanh Q, Kim, Jungwon, Kwangyun Jung, Khanh Q. Kieu, Jungwon Kim, Jung, Kwangyun   +7 more
core   +1 more source

Passive Q-Switching of a Diode-Pumped Pr:LiYF₄ Visible Laser Using WS₂ as Saturable Absorber

IEEE Photonics Journal, 2016
Q-switched laser operation of a blue-diode-pumped Pr:LiYF4 laser is demonstrated at 640 nm for the first time by using a tungsten disulfide (WS2) nanosheet material as a saturable absorber.
Yongjie Cheng, Jian Peng, Bin Xu, Han Yang, Zhengqian Luo, Huiying Xu, Zhiping Cai, Jian Weng   +7 more
doaj   +1 more source

Structural and biochemical insights into the thermostable esterase Ta0887 from Thermoplasma acidophilum

FEBS Open Bio, EarlyView.
In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey, Miriam Livier Llamas‐García, Gabriela M. Montero‐Morán, Leticia Santos, Samuel Lara‐González   +4 more
wiley   +1 more source

Identification and characterization of a gene conferring stress resistance in Escherichia coli and cyanobacteria

FEBS Open Bio, EarlyView.
Functional screening identified PcSyn14890, a cyanobacteria‐specific protein that enhances growth and stress resistance in E. coli and Synechocystis PCC6803. Although we expected it to function as a molecular chaperone, it was unable to protect against thermal aggregation of GAPDH.
Akiyo Yamada, Takaaki Tanaka, Manaka Uehara, Keigo Nakano, Ken Morishima, Rintaro Inoue, Masaaki Sugiyama, Masafumi Yohda   +7 more
wiley   +1 more source

Using fluorescence fluctuation spectroscopy to quantify molecular dynamics within cells

FEBS Open Bio, EarlyView.
Microscopy techniques like fluorescence correlation spectroscopy (FCS) and number and brightness analysis (N&B) allow to quantify concentrations, diffusion rates, and oligomerization of fluorescently labeled molecules in the living cell. In this protocol the principles of how to set up these types of experiments are described including calibration ...
Mark A. Hink
wiley   +1 more source

Low-dimensional saturable absorbers for ultrafast photonics in solid-state bulk lasers: status and prospects

Nanophotonics, 2020
Low-dimensional (LD) materials have originated a range of innovative applications in photonics and optoelectronics owning to their advantages of ultrafast carrier response and distinct nonlinear saturable absorption properties.
Hao Qianqian, Wang Cong, Liu Wenxin, Liu Xiaoqin, Liu Jie, Zhang Han   +5 more
doaj   +1 more source

Characterization of the key membrane protein cytochrome Cyc2 from Ghiorsea bivora

FEBS Open Bio, EarlyView.
This study reports the first detailed biochemical analysis of Cyc2TAG1_1, a cytochrome protein involved in microbial iron oxidation. We produced and purified this enzyme in E. coli, then measured its iron‐oxidizing efficiency, and characterized its electrical and structural properties.
Josiane Azizi, Alain Dolla, Frauke Baymann, Celine Rommevaux, Latifa Elantak, Helene Gaussier, Matthieu Nouailler   +6 more
wiley   +1 more source

Mechanochemical Synthesis and Characterization of Nanostructured ErB4 and NdB4 Rare‐Earth Tetraborides

Advanced Engineering Materials, Volume 27, Issue 6, March 2025.
ErB4 and NdB4 nanostructured powders are produced by mechanochemical synthesis. 5 h mechanical alloying and 4 M HCl acid leaching are used in the production. ErB4 and NdB4 powders exhibit maximum magnetization of 0.4726 emu g−1 accompanied with an antiferromagnetic‐to‐paramagnetic phase transition at about TN = 18 K and 0.132 emu g−1 with a maximum at ...
Burçak Boztemur, Faruk Kaya, Bora Derin, Mustafa Lütfi Öveçoğlu, Ju Li, Duygu Ağaoğulları   +5 more
wiley   +1 more source

GHz Compact Laser Enabled by GaSb Nanowires as Saturable Absorbers

Advanced Photonics Research, 2023
Owing to the narrow bandgap and excellent optoelectronic properties, III‐Sb nanowires (NWs) enable efficient optical response from near‐infrared to mid‐infrared, making them ideal candidates for broadband optical modulation.
Yanxue Yin, Genglin Li, Zixu Sa, Fengjing Liu, Shuo Sun, Xiaoli Sun, Yuechen Jia, Zai-Xing Yang   +7 more
doaj   +1 more source