Results 131 to 140 of about 9,619,631 (286)

Paclitaxel induces NM2‐dependent cellular contraction through GEF‐H1 dissociation from microtubules and RhoA/ROCK activation in cancer cells

open access: yesMolecular Oncology, EarlyView.
Taxanes are widely used chemotherapeutics whose effects on cellular mechanics remain poorly understood. We show that paclitaxel induces rapid cellular contraction by promoting GEF‐H1 dissociation from microtubules and non‐muscle myosin II activation through RhoA/ROCK.
Gloria Asensio‐Juárez   +5 more
wiley   +1 more source

Development and testing of the Perceived Wellbeing in Outdoor Shared Spaces (PWOSS) scale

open access: yesWellbeing, Space and Society
Accessible and high quality shared outdoor open spaces, both built and natural, can be particularly beneficial for health and wellbeing. The design and quality of shared outdoor spaces are often modifiable and the health and wellbeing-promoting potential
Craig W. McDougall   +5 more
doaj   +1 more source

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

open access: yesFEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz   +6 more
wiley   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

open access: yesFEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei   +3 more
wiley   +1 more source

Working the space: locating teachers’ voices in large-scale, mandated curriculum reform [PDF]

open access: yes, 2016
The educational landscape is one of significant change. This thesis is concerned with the early responses of a group of teachers to large-scale, mandated curriculum reform at one independent P-12 school located in regional Queensland.
Evans, Donna Leigh
core  

Submersible Space Training Suit Preliminary Test Results and Review

open access: yes
Immediate accessThis item is made available by the University of Arizona Center for Human Space Exploration (CHaSE) with support from the University of Arizona Libraries.
Smith, Cameron, Tresch, Trent
core   +4 more sources

Detection of Facial Features in Scale-Space

open access: yesActa Polytechnica, 2007
This paper presents a new approach to the detection of facial features. A scale adapted Harris Corner detector is used to find interest points in scale-space. These points are described by the SIFT descriptor. Thus invariance with respect to image scale,
P. Hosten, M. Asbach
doaj  

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

open access: yesFEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill   +4 more
wiley   +1 more source

A Deep Learning–Based Approach for Dielectric Permittivity Inversion of RIMFAX Ground-penetrating Radar Data

open access: yesThe Astronomical Journal
The dielectric permittivity is a crucial parameter in planetary ground-penetrating radar (GPR) missions, such as the RIMFAX radar onboard the Mars 2020 Perseverance rover.
Jun Zhang, Long Xiao, Yuefeng Yuan
doaj   +1 more source

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