Results 241 to 250 of about 10,082,734 (310)

GPR30 Functions as a Shared Inhibitory Signal for Both Pericyte-Mediated Angiogenesis and Oligodendrocyte Myelination. [PDF]

open access: yesGlia
Hui SW   +12 more
europepmc   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

open access: yesFEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill   +4 more
wiley   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

open access: yesFEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee   +3 more
wiley   +1 more source

Scale-dependent biases in systematic land cover maps undermine freshwater ecological assessment. [PDF]

open access: yesEnviron Monit Assess
Fernández de Larrea I   +3 more
europepmc   +1 more source

Ten-Year Surveillance of Microbiological Non-Compliance in Pork: Associations with Season and Slaughterhouse Scale in Lower Northern Thailand. [PDF]

open access: yesFoods
Mapongpeng R   +7 more
europepmc   +1 more source

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