Results 141 to 150 of about 3,865,865 (314)
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
FEBS Open Bio, EarlyView.ERα splice variant ERα∆7 lacks the C‐terminus, and its expression may change phenotypes of breast cancers. Our results showed that ERα∆7 is found in the luminal A subtype, and elevated ERα∆7 levels are linked to improved cell survival with lower proliferation and migration.
Long Wai Tsui +10 more
wiley +1 more source
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
FEBS Open Bio, EarlyView.Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
Decomposing Total Factor Productivity Change of Cotton Cultivars (Barakat-90 and Barac (67)B) in the Gezira Scheme (1991 – 2007) Sudan [PDF]
The main objective of this paper was to decompose Total Factor Productivity Change (TFPCH) of cotton cultivars Barakat-90 and Barac(67)B in the Gezira scheme in 1991-2007, based on Data Envelopment Analysis Program (DEAP) Software Version 2.1, using ...
Barakat, Hoyam E., Bushara, Mohamed O.A.
core +1 more source
FEBS Open Bio, EarlyView.Matrix metalloproteinase‐9 (MMP9) drives ovarian cancer progression. Using MMP9‐null cells (M9‐KO) created from ovarian cancer cells, we found MMP9 loss did not block Epidermal Growth Factor (EGF)‐driven E‐cadherin dissolution or EMT but delayed and reduced EGF‐driven membrane protrusions. Transient MMP9 re‐expression drove membrane protrusion.
Claire Strauel +8 more
wiley +1 more source
The Efficiency of China’s Carbon Trading Schemes: A Tale of Seven Pilot Markets
CommoditiesThis study evaluates the efficiency of China’s seven emission trading schemes (ETS) piloted in 2013. We evaluate seven pilots’ overall technical and scale efficiencies and temporal dynamics during 2014–2023. We use a bootstrap correction data envelopment
Yigang Wei +3 more
doaj +1 more source
Efficiency of coordinate descent methods on huge-scale optimization problems [PDF]
In this paper we propose new methods for solving huge-scale optimization problems. For problems of this size, even the simplest full-dimensional vector operations are very expensive.
NESTEROV, Yurii
core
FEBS Open Bio, EarlyView.This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel +4 more
wiley +1 more source
Technical and scale efficiency of provincial health systems in China: a bootstrapping data envelopment analysis. [PDF]
BMJ Open, 2019 Chai P, Zhang Y, Zhou M, Liu S, Kinfu Y.
europepmc +1 more source
Inducing Efficiency in Oligopolistic Markets with Increasing Returns to Scale [PDF]
We consider a Cournot Oligopoly market of firms possessing increasing returns to scale technologies. It is shown that an external regulating agency can increase total social welfare without running a deficit.
Abhijit Sengupta, Yair Tauman
core